LOCUS       sequence-320027-         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express miR-144 under the U6 promoter in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pU6-mir144 EF1Alpha-puro-T2A-BFP
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     18..38
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        351..562
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 ttatacgaag ttataaacag cacaaaagga aactcaccct aactgtaaag taattgtgtg
       61 ttttgagact ataagtatcc cttggagaac caccttgttg ggttctgccc ccagcccctc
      121 acagtgcttt tcaagccatg cttcctgtgc ccccagtggg gccctggctg ggatatcatc
      181 atatactgta agtttgcgat gagacactac agtatagatg atgtactagt ccgggcaccc
      241 ccagctctgg agcctgacaa ggaggacagg agagatgctg cctcgagtac taggatccat
      301 taggcggccg cgtggataac cgtattaccg ccatgcatgt gcccgtcagt gggcagagcg
      361 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta
      421 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
      481 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
      541 cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt
      601 tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt acgtgattct
      661 tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaangagc
      721 cccttcgcct cgtgcttgag ttgaggcctg gcctg
//
