LOCUS       sequence-320066-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For CRISPR knockout of miR-144~451 linker region by lentiviral 
            delivery of Cas9 and linker gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pLentiCRISPRv1.linker_KO
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(48..296)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(106..125)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(276..296)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(474..518)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tttacttgct atttctagct ctaaaacaag aagctctctg ctcagccggt gtttcgtcct
       61 ttccacaaga tatataaagc caagaaatcg aaatactttc aagttacggt aagcatatga
      121 tagtccattt taaaacataa ttttaaaact gcaaactacc caagaaatta ttactttcta
      181 cgtcacgtat tttgtactaa tatctttgtg tttacagtca aattaattct aattatctct
      241 ctaacagcct tgtatcgtat atgcaaatat gaaggaatca tgggaaatag gccctctctg
      301 ggtcccctcg gggttgggag gtgggtctga aacgataatg gtgaatatcc ctgcctaact
      361 ctattcacta tagaaagtac agcaaaaact attcttaaac ctaccaagcc tcctactatc
      421 attatgaata attttatata ccacagccaa tttgttatgt taaaccaatt ccacaaactt
      481 gcccatttat ctaattccaa taattcttgt tcattctttt cttgctggtt ttgcgattct
      541 tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca ctccatccag
      601 gtcgtgtgat tccaaatctg ttccagagat ttattactcc aactagcatt ccaaggcaca
      661 gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc ccaggagctg ttgatccttt
      721 aggtatcttt ccacagccag gattcttgcc tggagctgct tgatgcccca gactgtgagt
      781 tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg ctgcactata
      841 ccagacaata attgtctggc ctgtaccgtc
//
