LOCUS       sequence-320093-       11381 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This insert, when expressed in the same cell with other split Cre 
            component, forms Cre recombinase through protein trans-splicing 
            using split intein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11381)
  AUTHORS   Luan H, Kuzin A, Odenwald WF, White BH
  TITLE     Cre-assisted fine-mapping of neural circuits using orthogonal split 
            inteins.
  JOURNAL   Elife. 2020 Apr 14;9. pii: 53041. doi: 10.7554/eLife.53041.
  PUBMED    32286225
REFERENCE   2  (bases 1 to 11381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-04-14"; volume: "9. pii: 53041. doi"; pages: " 
            10.7554/eLife.53041"
COMMENT     Sequence Label: HJP-473-DNE>FLP-T2A-CreAB-NrdJ-1N
FEATURES             Location/Qualifiers
     source          1..11381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1244..1268)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1445..1478)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             2772..2783
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2775..2828
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    complement(3903..3936)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    3945..4533
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3998..4018)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4416..4427)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4794..4810)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4794..4810)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(5821..6435,6506..6637,6841..7156,7218..7872,
                     7947..8220,8622..8693))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     9145..9163
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    9388..9457
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(9544..10132)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9624..9643)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10303..11094,11095..11163))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10926..10945
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11164..11268)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11336..11354
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 acaagtttgt acaaaaaagc aggctccgcg gccgccccct tcacctcact ttgcacaacc
       61 agggttgcca ggcggcgcaa tacgtttacg atcccaccca aaactcccca aatcggtcca
      121 taggtagatc tacgtcagct gtcagctggc gcatggcccg cgtcagtggt tattgacaac
      181 tcctttggtc gtaatcctgc caacatcgta aagtaataaa tttgcctgaa aaaggaaaat
      241 ctgccgggga tcgactataa aaattggccc agccaggtaa gctccgtaat gattgacccc
      301 gaccctcagt cccagaccca cgaccgatcc aggtccgatt ccgatccatt gcaagtcgcg
      361 attccgtccc gaaacccaag caaccccctt aagacgcttc tgccgatgag ccggccaggc
      421 aaaagcgaag aatttgaaac taaggatttc gccttgcaag ggggttgggt tgtcacgggg
      481 gttgcggcag ctgcgccgaa caattgccaa ggagttctcc ttaatgaatt gcaaatcatt
      541 cgccgggcat tgatcaagtt tgtccttgcc aagggtgaaa tctgtgcgac taaatgtgcc
      601 atcagccagc cacccaaaag ggaaacggac acaaacggaa catctgtcgc aagaattcgc
      661 ccttgtgtct gctagtctgt tagtctgtta cctaccccac caaaagcgaa cttgacgagc
      721 ttagtcgtct gaggctgata aatgcagtgc cgcaaagtac acctcgcgaa atttgtgtat
      781 gagatgtatg aaaaatgtaa aaatagatag caatccgaac tatgctccat gctaatgttc
      841 ttcccagccg ttattacttg ttacattgtt tacttttata accatcttgg ccaacagctg
      901 tttagcacca aatgccaaca aagaaacaat tcaccttttg gcccaaggat attcccgatc
      961 tattgatgaa ccttatgcct acatgctcaa ttaacgactc catgggtgtc agctgttttt
     1021 ggcaaaaata aggacaatgt actgctcaga tttccgctga atacacaaac atacatacat
     1081 atttgcctag gaaggtgctg cgattttggt gcgtggcaat tttgggtacc gaaacaacag
     1141 ctgtcgcaca ttgatttctc cgagtgcatt gtcctggccg caaaacggga gactgtgtgt
     1201 gtgtgtgcgt aggactttca ctccgtgaag ggtgggcgcg ccgacccagc tttcttgtac
     1261 aaagtggtga taaacggccg gccgagctcg cccggggatc gagcgcagcg gtataaaagg
     1321 gcgcggggtg gctgagagca tcagttgtga atgaatgttc gagccgagca gacgtgccgc
     1381 tgccttcgtt aatatccttt gaataagcca actttgaatc acaagacgca taccaaacgg
     1441 taccgaagtt cctatacttt ctagagaata ggaacttcgc tagcaactta aaaaaaaaaa
     1501 tcaaaatgcc ccagttcggc atcctgtgca agaccccccc aaaggtgctg gtgcgccagt
     1561 tcgtggagcg cttcgagcgc cccagcggcg agaagattgc cctgtgcgcc gccgagctga
     1621 cctacctgtg ctggatgatc acccacaacg gcaccgccat caagcgcgcc accttcatga
     1681 gctacaacac catcatcagc aacagcctga gcttcgacat cgtgaacaag agcctgcagt
     1741 tcaagtacaa gacccagaag gccaccatcc tggaggccag cctgaagaag ctgatccccg
     1801 cctgggagtt caccatcatc ccgtactacg gccagaagca ccagagcgat atcaccgata
     1861 tcgtgtccag cctgcagctg cagttcgaga gcagcgagga ggccgataag ggcaacagcc
     1921 acagcaagaa gatgctgaag gccctgctga gcgagggcga gagcatctgg gagatcaccg
     1981 agaagatcct gaacagcttc gagtacacca gccgcttcac caagaccaag accctgtacc
     2041 agttcctgtt cctggccacc ttcatcaact gcggccgctt cagcgatatc aagaacgtgg
     2101 accccaagag cttcaagctg gtgcagaaca agtacctggg cgtgattatc cagtgcctgg
     2161 tgaccgagac caagacgagc gtgtcccgcc acatctactt cttcagcgcc cgcggacgca
     2221 ttgatccgct ggtgtacctg gatgagttcc tgcgcaacag cgagccagtg ctgaagcgcg
     2281 tgaaccgcac cggcaacagc agcagcaaca agcaggagta ccagctgctg aaggataacc
     2341 tggtgcgcag ctacaacaag gccctgaaga agaacgcccc ctacagcatc ttcgccatca
     2401 agaacggccc caagtcccac attggccgcc acctgatgac ctcgttcctg agcatgaagg
     2461 gcctgaccga gctgacgaac gtggtgggca attggagcga taagcgcgcc agcgccgtgg
     2521 cccgcaccac ctacacccac cagatcaccg ccatccccga tcactacttc gccctggtgt
     2581 cccgctacta cgcctacgat cccatcagca aggagatgat cgccctgaag gatgagacca
     2641 accccatcga ggagtggcag cacatcgagc agctgaaggg cagcgccgag ggcagcattc
     2701 gctaccccgc ctggaacggc atcatcagcc aggaggtgct ggattacctg agcagctaca
     2761 tcaaccgccg cattgagggc cgcggcagcc tgctgacctg cggcgatgtg gaggagaacc
     2821 ccgggcccat gagtaacttg ctcacggtac accaaaattt gccggccctg cctgtggacg
     2881 ccacgtccga cgaggtccga aagaatctga tggacatgtt ccgtgacagg caggccttta
     2941 gcgagcacac ctggaagatg ctgctctcgg tgtgcaggtc ctgggccgct tggtgcaagc
     3001 tgaacaaccg aaagtggttt cccgcggagc ccgaggacgt ccgcgattat ctgctgtacc
     3061 tgcaggctcg cggactggcc gtcaaaacaa tacagcaaca tttgggtcag ctgaacatgc
     3121 tccacaggcg cagcggactg ccccggccat ccgatagcaa tgcagtgagc ctggtaatgc
     3181 gtcgaatacg caaggaaaac gtggacgccg gcgagagggc caaacaggcg ttggctttcg
     3241 aacgcaccga tttcgatcag gtgcggtcgt tgatggagaa cagcgatcga tgccaggaca
     3301 tccggaactt ggcgtttttg ggcattgcct ataacaccct ccttcgcatc gccgagatag
     3361 cgcgtatccg agttaaagac atttcccgta cagacggagg ccgcatgctt attcacatcg
     3421 gacgcacgaa aaccctggtc agcactgctg gcgtggagaa agcactttcc ctgggtgtga
     3481 cgaaattggt cgagcgatgg atcagtgtga gtggcgtcgc cgatgacccc aacaattatc
     3541 tgttctgcag ggtgcgcaaa aatggagtgg cagccccctg cctggtgggc agcagcgaga
     3601 tcatcacccg caactacggc aagaccacca tcaaggaggt ggtggagatc ttcgataacg
     3661 ataagaacat ccaggtgctg gccttcaaca cccacaccga taacatcgag tgggccccca
     3721 tcaaggccgc ccagctgacc cgccccaacg ccgagctggt ggagctggag atcgataccc
     3781 tgcacggcgt gaagaccatc cgctgcaccc ccgatcaccc cgtgtacacc aagaaccgcg
     3841 gctacgtgcg cgccgatgag ctgaccgatg atgatgagct ggtggtggcc atctaaaccg
     3901 gtgaagttcc tatactttct agagaatagg aacttcgttt aaacaatcaa cctctggatt
     3961 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     4021 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     4081 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     4141 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     4201 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     4261 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     4321 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     4381 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     4441 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     4501 cgagtcggat ctccctttgg gccgcctccc cgcctgaagc ttatcgatac cgtcgactaa
     4561 agccaaatag aaaattattc agttcctggc ttaagttttt aaaagtgata ttatttattt
     4621 ggttgtaacc aaccaaaaga atgtaaataa ctaatacata attatgttag ttttaagtta
     4681 gcaacaaatt gattttagct atattagcta cttggttaat aaatagaata tatttattta
     4741 aagataattg cgtttttatt gtcagggagt gagtttgctt aaaaactcgt ttagatccac
     4801 tagttctaga gcggccgctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc
     4861 cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga
     4921 acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc
     4981 ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc
     5041 cggaggcgcg ccctagttcc agtgaaatcc aagcattttc taaattaaat gtattcttat
     5101 tattatagtt gttatttttg atatatataa acaacactat tatgcccacc atttttttga
     5161 gatgcatcta cacaaggaac aaacactgga tgtcactttc agttcaaatt gtaacgctaa
     5221 tcactccgaa caggtcacaa aaaattacct taaaaagtca taatattaaa ttagaataaa
     5281 tatagctgtg agggaaatat atacaaatat attggagcaa ataaattgta catacaaata
     5341 tttattacta atttctattg agacgaaatg aaccactcgg aaccatttga gcgaaccgaa
     5401 tcgcgcggaa ctaacgacag tcgctccaag gtcgtcgaac aaaaggtgaa tgtgttgcgg
     5461 agagcgggtg ggagacagcg aaagagcaac tacgaaacgt ggtgtggtgg aggtgaatta
     5521 tgaagagggc gcgcgatttg aaaagtatgt atataaaaaa tatatcccgg tgttttatgt
     5581 agcgataaac gagtttttga tgtaaggtat gcaggtgtgt aagtcttttg gttagaagac
     5641 aaatccaaag tctacttgtg gggatgttcg aaggggaaat acttgtattc tataggtcat
     5701 atcttgtttt tattggcaca aatataatta cattagcttt ttgagggggc aataaacagt
     5761 aaacacgatg gtaataatgg taaaaaaaaa aaacaagcag ttatttcgga tatatgtcgg
     5821 ctactccttg cgtcgggccc gaagtcttag agccagatat gcgagcaccc ggaagctcac
     5881 gatgagaatg gccagaccca cgtagtccag cggcagatcg gcggcggaga agttaagcgt
     5941 ctccaggatg accttgcccg aactggggca cgtggtgttc gacgatgtgc agctaatttc
     6001 gcccggctcc acgtccgccc attggttaat cagcagaccc tcgttggcgt aacggaacca
     6061 tgagaggtac gacaaccatt tgaggtatac tggcaccgag cccgagttca agaagaagcc
     6121 gccaaagagc aggaatggta tgataaccgg cggacccaca gacagcgcca tcgaggtcga
     6181 ggagctggcg caggatatta gatatccgaa ggacgttgac acattggcca ccagagtgac
     6241 cagcgccagg cagttgaaga agtgcagcac tccggcccgc agtccgatca tcggataggc
     6301 aatcgccgtg aagaccagtg gcactgtgag aaaaagcggc aattcggcaa tcgttttgcc
     6361 cagaaagtat gtgtcacagc gataaagtcg acttcgggcc tccctcataa aaactggcag
     6421 ctctgaggtg aacacctaaa tcgaatcgat tcattagaaa gttagtaaat tattgaaatg
     6481 caaatgtatt ctaaacatga cttacattta tcgtggcaaa gacgttttga aaggtcatgt
     6541 tggtcaggaa gaggaagatg gctccgttga tattcatcac acccacttgc gtgagttgtt
     6601 ggcccaaaaa gatgaggcca atcaagatgg caaccatctg caaattaaaa tgttactcgc
     6661 atctcattaa tattcgcgag ttaaatgaaa tttatttatc ttctgcaaaa ctataaacta
     6721 tacatctcat tgaaaaaaac taagaagggt gtggaatcag gcaattctat ctaaaatcta
     6781 gcgaatttgt ttccaagaat tgtaagcgtt atatcatttg tttccactgg aaccactcac
     6841 cgttgtctga ataagtcgca cttttacgag gagtggttcc ttgagcaccg acagccagga
     6901 tcgccacagg accgcccgga actgcatgaa ccaggtggcc ttgtaggtgt acccattctc
     6961 cggctgctcc agtggcttct ccagattttt ggtggccaac aactgctcca tatcccgggc
     7021 tactttgcta atggcaaaat tgtcgcatat cttggcgatc cgatcacggg actcgatctc
     7081 ccgtccgggc acaacggcca acacctgtac gtaaaagtcc gccggattgt agttggtagg
     7141 acactgggca cccacgctgg ataggagttg agatgttatg taatactaga tacccttaat
     7201 aaacacatcg aactcactag gaaaagaagt cgacggcttc gctgggagtg cccaagaaag
     7261 ctaccctgcc ctcggccatc agaaggatct tgtcaaagag ctcaaacagc tcggaagacg
     7321 gctgatgaat ggtcaggatg acggtcttgc ccttctgcga cagcttcttc agcacctgga
     7381 cgacgctgtg ggcggtaaag gagtccagtc cggaggtggg ctcatcgcag atcagaagcg
     7441 gcggatcggt tagagcctcg gaggcgaatg ccagacgctt cctttctccg ccggacagac
     7501 ctttcaccct gccgggcaca ccgatgatcg tgtgctgaca tttgctgagc gaaagctcct
     7561 ggatcacctg atccacgcgg gccactcgct gccgataggt cagatgtcgt ggcatccgca
     7621 ccatggcttg gaaaatcagg tgttccctgg ccgttaggga gccgataaag aggtcatcct
     7681 gctggacata ggcgcacctg gcctgcatct ccttggcgtc cacaggttgg ccattgagca
     7741 gtcgcatccc ggatggcgat acttggatgc cctgcggcga tcgaaaggca agggcattca
     7801 gcagggtcgt ctttccggca ccggaactgc ccatcacggc caaaagttcg cccggatagg
     7861 ccacgccgca aactgagttt caaattggta attggaccct ttattaagat ttcacacaga
     7921 tcagccgact gcgaatagaa actcaccgtt cttgagcaaa tgtttcctgg gcgccggtat
     7981 gtgtcgctcg ttgcagaata gtccgcgtgt ccggttgacc agctgccgcc atccggagcc
     8041 cggctgattg accgccccaa agatgtccat attgtgccag gcataggtga ggttctcggc
     8101 tagttggccg ctccctgaac cggagtcctc cggcggactg ggtggccgga gcgtgccgta
     8161 gtttttggcc tgcccgaagc cctggttaat gcagctctgc gaagccgctc cgctgtcacc
     8221 ctgcaatgat aggggatctc aaatatcaac tacaagcgtt atgctcatct aaccccgaac
     8281 aaaaagtacc ccgaagtatc ctacgaagta ggtttatact tttatttatt ttttgtgcat
     8341 ctaggatcag cttaaaatat ctggttgtta tattttttgt aaaaaagaat atagtcgaaa
     8401 atgaatgcct ttagatgtct tgatcatgat atgatctcaa aaattgtctt atatagcgag
     8461 aacagctacc agaataatct gtttcgtgtc actatttgtt tgtgcaattg cggtttggga
     8521 tttttgtggg tcgcagttct cacgccgcag acaatttgat gttgcaatcg cagttcctat
     8581 agatcaagtg aacttaagat gtatgcacat gtactactca cattgttcag atgctcggca
     8641 gatgggtgtt tgctgcctcc gcgaattaat agctcctgat cctcttggcc cattgccggg
     8701 atttttcaca ctttcccctg cttacccacc caaaaccaat caccacccca atcactcaaa
     8761 aaacaaacaa aaataagaag cgagaggagt tttggcacag cactttgtgt ttaattgatg
     8821 gcgtaaaccg cttggagctt cgtcacgaaa ccgctgacaa aatgcaactg aaggcggaca
     8881 ttgacgctac gtaacgctac aaacggtggc gaaagagata gcggacgcag cggcgaaaga
     8941 gacggcgata tttctgtgga cagagaagga ggcaaacagc gctgactttg agtggaatgt
     9001 cattttgagt gagaggtaat cgaaagaacc tggtacatca aatacccttg gatcgaagta
     9061 aatttaaaac tgatcagata agttcaatga tatccagtgc agtaaaaaaa aaaaatgttt
     9121 tttttatcta ctttccgcaa aaatgggttt tattaactta catacatact aggcgcgccc
     9181 atatgttcgg cttgtcgaca tgcccgccgt gaccgtcgag aacccgctga cgctgccccg
     9241 cgtatccgca cccgccgacg ccgtcgcacg tcccgtgctc accgtgacca ccgcgcccag
     9301 cggtttcgag ggcgagggct tcccggtgcg ccgcgcgttc gccgggatca actaccgcca
     9361 cctcgacccg ttcatcatga tggaccagat gggtgaggtg gagtacgcgc ccggggagcc
     9421 caagggcacg ccctggcacc cgcaccgcgg cttcgagacc gtgacctaca tcgtcgacgg
     9481 taacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     9541 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     9601 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     9661 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     9721 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9781 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     9841 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     9901 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9961 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
    10021 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    10081 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    10141 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    10201 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    10261 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    10321 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    10381 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    10441 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    10501 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    10561 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    10621 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    10681 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    10741 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    10801 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    10861 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    10921 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    10981 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    11041 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    11101 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    11161 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    11221 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    11281 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
    11341 gcgtatcacg aggccctttc gtcttcaaga attcgtttat c
//
