LOCUS       sequence-320103-        9882 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This insert, when expressed in the same cell with other split Cre 
            component, forms Cre recombinase through protein trans-splicing 
            using split intein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9882)
  AUTHORS   Luan H, Kuzin A, Odenwald WF, White BH
  TITLE     Cre-assisted fine-mapping of neural circuits using orthogonal split 
            inteins.
  JOURNAL   Elife. 2020 Apr 14;9. pii: 53041. doi: 10.7554/eLife.53041.
  PUBMED    32286225
REFERENCE   2  (bases 1 to 9882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-04-14"; volume: "9. pii: 53041. doi"; pages: " 
            10.7554/eLife.53041"
COMMENT     Sequence Label: HJP-176-IVS-Syn21-CreA-gp41-1N-WPREw
FEATURES             Location/Qualifiers
     source          1..9882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(103..108,109..126,127..133)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             187..846
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(253..272)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1188..1493
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1372..1391
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1534..1658)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          1697..1763
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     misc_feature    2400..2988
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2453..2473)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2871..2882)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3249..3265)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3249..3265)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(4276..4890,4961..5092,5296..5611,5673..6327,
                     6402..6675,7077..7148))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     7600..7618
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    7843..7912
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(7999..8587)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8079..8098)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8758..9549,9550..9618))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9381..9400
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9619..9723)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9790..9808
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    join(9836..9882,1..78)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca
       61 acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc
      121 ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa
      181 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
      241 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
      301 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
      361 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa
      421 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
      481 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
      541 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
      601 attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta
      661 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg
      721 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc
      781 ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg
      841 gcgtaaacgc gtggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct
      901 gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt
      961 atgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg
     1021 catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg
     1081 tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt
     1141 tattgaaatg aacggctctt ttgctgacga gaacaggggc tggtgaaatg cagtttaagg
     1201 tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta
     1261 ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata
     1321 aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga
     1381 ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc
     1441 accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag
     1501 gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta
     1561 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca
     1621 ttttacgttt ctcgttcagc tttcttgtac aaagtggtga taaacggccg gccagatctg
     1681 gtaccgctag aaaaaggtag gttcaaccac tgatgcctag gcacaccgaa acgactaacc
     1741 ctaattctta tcctttactt cagggctagc aacttaaaaa aaaaaatcaa aatgagtaac
     1801 ttgctcacgg tacaccaaaa tttgccggcc ctgcctgtgg acgccacgtc cgacgaggtc
     1861 cgaaagaatc tgatggacat gttccgtgac aggcaggcct ttagcgagca cacctggaag
     1921 atgctgctct cggtgtgcag gtcctgggcc gcttggtgca agctgaacaa ccgaaagtgg
     1981 tttcccgcgg agcccgagga cgtccgcgat tatctgctgt acctgcaggc tcgcggactg
     2041 gccgtcaaaa caatacagca acatttgggt cagctgaaca tgctccacag gcgcagcgga
     2101 ctgccccggc catccgattg cctggatctg aagacccagg tgcagacccc ccagggcatg
     2161 aaggagatca gcaacatcca ggtgggcgat ctggtgctga gcaacaccgg ctacaacgag
     2221 gtgctgaacg tgttccccaa gagcaagaag aagagctaca agatcaccct ggaggatggc
     2281 aaggagatca tctgcagcga ggagcacctg ttccccaccc agaccggcga gatgaacatc
     2341 agcggcggcc tgaaggaggg catgtgcctg tacgtgaagg agtaaaccgg tgtttaaaca
     2401 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     2461 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     2521 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     2581 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     2641 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     2701 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     2761 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     2821 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     2881 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     2941 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct gaagcttatc
     3001 gataccgtcg actaaagcca aatagaaaat tattcagttc ctggcttaag tttttaaaag
     3061 tgatattatt tatttggttg taaccaacca aaagaatgta aataactaat acataattat
     3121 gttagtttta agttagcaac aaattgattt tagctatatt agctacttgg ttaataaata
     3181 gaatatattt atttaaagat aattgcgttt ttattgtcag ggagtgagtt tgcttaaaaa
     3241 ctcgtttaga tccactagtt ctagagcggc cgctggccac gggtgcgcat gatcgtgctc
     3301 ctgtcgttga ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca
     3361 ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca
     3421 acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc
     3481 tgcaccatta tgttccggag gcgcgcccta gttccagtga aatccaagca ttttctaaat
     3541 taaatgtatt cttattatta tagttgttat ttttgatata tataaacaac actattatgc
     3601 ccaccatttt tttgagatgc atctacacaa ggaacaaaca ctggatgtca ctttcagttc
     3661 aaattgtaac gctaatcact ccgaacaggt cacaaaaaat taccttaaaa agtcataata
     3721 ttaaattaga ataaatatag ctgtgaggga aatatataca aatatattgg agcaaataaa
     3781 ttgtacatac aaatatttat tactaatttc tattgagacg aaatgaacca ctcggaacca
     3841 tttgagcgaa ccgaatcgcg cggaactaac gacagtcgct ccaaggtcgt cgaacaaaag
     3901 gtgaatgtgt tgcggagagc gggtgggaga cagcgaaaga gcaactacga aacgtggtgt
     3961 ggtggaggtg aattatgaag agggcgcgcg atttgaaaag tatgtatata aaaaatatat
     4021 cccggtgttt tatgtagcga taaacgagtt tttgatgtaa ggtatgcagg tgtgtaagtc
     4081 ttttggttag aagacaaatc caaagtctac ttgtggggat gttcgaaggg gaaatacttg
     4141 tattctatag gtcatatctt gtttttattg gcacaaatat aattacatta gctttttgag
     4201 ggggcaataa acagtaaaca cgatggtaat aatggtaaaa aaaaaaaaca agcagttatt
     4261 tcggatatat gtcggctact ccttgcgtcg ggcccgaagt cttagagcca gatatgcgag
     4321 cacccggaag ctcacgatga gaatggccag acccacgtag tccagcggca gatcggcggc
     4381 ggagaagtta agcgtctcca ggatgacctt gcccgaactg gggcacgtgg tgttcgacga
     4441 tgtgcagcta atttcgcccg gctccacgtc cgcccattgg ttaatcagca gaccctcgtt
     4501 ggcgtaacgg aaccatgaga ggtacgacaa ccatttgagg tatactggca ccgagcccga
     4561 gttcaagaag aagccgccaa agagcaggaa tggtatgata accggcggac ccacagacag
     4621 cgccatcgag gtcgaggagc tggcgcagga tattagatat ccgaaggacg ttgacacatt
     4681 ggccaccaga gtgaccagcg ccaggcagtt gaagaagtgc agcactccgg cccgcagtcc
     4741 gatcatcgga taggcaatcg ccgtgaagac cagtggcact gtgagaaaaa gcggcaattc
     4801 ggcaatcgtt ttgcccagaa agtatgtgtc acagcgataa agtcgacttc gggcctccct
     4861 cataaaaact ggcagctctg aggtgaacac ctaaatcgaa tcgattcatt agaaagttag
     4921 taaattattg aaatgcaaat gtattctaaa catgacttac atttatcgtg gcaaagacgt
     4981 tttgaaaggt catgttggtc aggaagagga agatggctcc gttgatattc atcacaccca
     5041 cttgcgtgag ttgttggccc aaaaagatga ggccaatcaa gatggcaacc atctgcaaat
     5101 taaaatgtta ctcgcatctc attaatattc gcgagttaaa tgaaatttat ttatcttctg
     5161 caaaactata aactatacat ctcattgaaa aaaactaaga agggtgtgga atcaggcaat
     5221 tctatctaaa atctagcgaa tttgtttcca agaattgtaa gcgttatatc atttgtttcc
     5281 actggaacca ctcaccgttg tctgaataag tcgcactttt acgaggagtg gttccttgag
     5341 caccgacagc caggatcgcc acaggaccgc ccggaactgc atgaaccagg tggccttgta
     5401 ggtgtaccca ttctccggct gctccagtgg cttctccaga tttttggtgg ccaacaactg
     5461 ctccatatcc cgggctactt tgctaatggc aaaattgtcg catatcttgg cgatccgatc
     5521 acgggactcg atctcccgtc cgggcacaac ggccaacacc tgtacgtaaa agtccgccgg
     5581 attgtagttg gtaggacact gggcacccac gctggatagg agttgagatg ttatgtaata
     5641 ctagataccc ttaataaaca catcgaactc actaggaaaa gaagtcgacg gcttcgctgg
     5701 gagtgcccaa gaaagctacc ctgccctcgg ccatcagaag gatcttgtca aagagctcaa
     5761 acagctcgga agacggctga tgaatggtca ggatgacggt cttgcccttc tgcgacagct
     5821 tcttcagcac ctggacgacg ctgtgggcgg taaaggagtc cagtccggag gtgggctcat
     5881 cgcagatcag aagcggcgga tcggttagag cctcggaggc gaatgccaga cgcttccttt
     5941 ctccgccgga cagacctttc accctgccgg gcacaccgat gatcgtgtgc tgacatttgc
     6001 tgagcgaaag ctcctggatc acctgatcca cgcgggccac tcgctgccga taggtcagat
     6061 gtcgtggcat ccgcaccatg gcttggaaaa tcaggtgttc cctggccgtt agggagccga
     6121 taaagaggtc atcctgctgg acataggcgc acctggcctg catctccttg gcgtccacag
     6181 gttggccatt gagcagtcgc atcccggatg gcgatacttg gatgccctgc ggcgatcgaa
     6241 aggcaagggc attcagcagg gtcgtctttc cggcaccgga actgcccatc acggccaaaa
     6301 gttcgcccgg ataggccacg ccgcaaactg agtttcaaat tggtaattgg accctttatt
     6361 aagatttcac acagatcagc cgactgcgaa tagaaactca ccgttcttga gcaaatgttt
     6421 cctgggcgcc ggtatgtgtc gctcgttgca gaatagtccg cgtgtccggt tgaccagctg
     6481 ccgccatccg gagcccggct gattgaccgc cccaaagatg tccatattgt gccaggcata
     6541 ggtgaggttc tcggctagtt ggccgctccc tgaaccggag tcctccggcg gactgggtgg
     6601 ccggagcgtg ccgtagtttt tggcctgccc gaagccctgg ttaatgcagc tctgcgaagc
     6661 cgctccgctg tcaccctgca atgatagggg atctcaaata tcaactacaa gcgttatgct
     6721 catctaaccc cgaacaaaaa gtaccccgaa gtatcctacg aagtaggttt atacttttat
     6781 ttattttttg tgcatctagg atcagcttaa aatatctggt tgttatattt tttgtaaaaa
     6841 agaatatagt cgaaaatgaa tgcctttaga tgtcttgatc atgatatgat ctcaaaaatt
     6901 gtcttatata gcgagaacag ctaccagaat aatctgtttc gtgtcactat ttgtttgtgc
     6961 aattgcggtt tgggattttt gtgggtcgca gttctcacgc cgcagacaat ttgatgttgc
     7021 aatcgcagtt cctatagatc aagtgaactt aagatgtatg cacatgtact actcacattg
     7081 ttcagatgct cggcagatgg gtgtttgctg cctccgcgaa ttaatagctc ctgatcctct
     7141 tggcccattg ccgggatttt tcacactttc ccctgcttac ccacccaaaa ccaatcacca
     7201 ccccaatcac tcaaaaaaca aacaaaaata agaagcgaga ggagttttgg cacagcactt
     7261 tgtgtttaat tgatggcgta aaccgcttgg agcttcgtca cgaaaccgct gacaaaatgc
     7321 aactgaaggc ggacattgac gctacgtaac gctacaaacg gtggcgaaag agatagcgga
     7381 cgcagcggcg aaagagacgg cgatatttct gtggacagag aaggaggcaa acagcgctga
     7441 ctttgagtgg aatgtcattt tgagtgagag gtaatcgaaa gaacctggta catcaaatac
     7501 ccttggatcg aagtaaattt aaaactgatc agataagttc aatgatatcc agtgcagtaa
     7561 aaaaaaaaaa tgtttttttt atctactttc cgcaaaaatg ggttttatta acttacatac
     7621 atactaggcg cgcccatatg ttcggcttgt cgacatgccc gccgtgaccg tcgagaaccc
     7681 gctgacgctg ccccgcgtat ccgcacccgc cgacgccgtc gcacgtcccg tgctcaccgt
     7741 gaccaccgcg cccagcggtt tcgagggcga gggcttcccg gtgcgccgcg cgttcgccgg
     7801 gatcaactac cgccacctcg acccgttcat catgatggac cagatgggtg aggtggagta
     7861 cgcgcccggg gagcccaagg gcacgccctg gcacccgcac cgcggcttcg agaccgtgac
     7921 ctacatcgtc gacggtaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     7981 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8041 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8101 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8161 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8221 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8281 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8341 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8401 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     8461 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8521 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8581 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8641 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     8701 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     8761 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     8821 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     8881 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     8941 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9001 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9061 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9121 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9181 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9241 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9301 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9361 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9421 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9481 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9541 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9601 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     9661 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     9721 gcgcacattt ccccgaaaag tgccacctga gtctaagaaa ccattattat catgacatta
     9781 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaattcgt ttatcacaag
     9841 tttgtacaaa aaagctgaac gagaaacgta aaatgatata aa
//
