LOCUS       sequence-320168-        7311 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pXI - 3 -LoxP -KlURA3 -pTEF1 - XylO_94 -yeGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7311)
  AUTHORS   Ambri F, D'Ambrosio V, Di Blasi R, Maury J, Jacobsen SAB, McCloskey 
            D, Jensen MK, Keasling JD
  TITLE     High-Resolution Scanning of Optimal Biosensor Reporter Promoters in 
            Yeast.
  JOURNAL   ACS Synth Biol. 2020 Feb 21;9(2):218-226. doi: 
            10.1021/acssynbio.9b00333. Epub 2020 Jan 31.
  PUBMED    31935067
REFERENCE   2  (bases 1 to 7311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00333"; journalName: "ACS Synth Biol"; date: 
            "2020-02-21- 21"; volume: "9"; issue: "2"; pages: "218-226"
COMMENT     Sequence Label: pXylO_94
FEATURES             Location/Qualifiers
     source          1..7311
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             271..987
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1035..1200
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1090..1114)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    complement(1854..1887)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(2094..2113)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(2119..2138)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        2180..2271
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2272..2340,2341..3132)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2490..2509)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3254..3842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3743..3762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3996..4013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     4112..4128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4598..4631
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     5255..5277
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5270..5286
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5296..5315
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5296..5314
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5358..5391)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(6843..6876)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(6924..7113)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
ORIGIN
        1 cgtttctttt tcttcgtcga aaaaggcaat aaaaattttt atcacgtttc tttttcttga
       61 aaattttttt ttttgatttt tttctctttc gatgacctcc cattgatatt taagttaata
      121 aacggtcttc aatttctcaa gacatgttag cgctaccaag ttttcagttt catttttctt
      181 gttctattac aacttttttt acttcttgct cattagaaag aaagcatagc aatctaatct
      241 aagttttaat tacaaagtgc aggtaaaaca atgtctaaag gtgaagaatt attcactggt
      301 gttgtcccaa ttttggttga attagatggt gatgttaatg gtcacaaatt ttctgtctcc
      361 ggtgaaggtg aaggtgatgc tacttacggt aaattgacct taaaatttat ttgtactact
      421 ggtaaattgc cagttccatg gccaacctta gtcactactt tcggttatgg tgttcaatgt
      481 tttgcgagat acccagatca tatgaaacaa catgactttt tcaagtctgc catgccagaa
      541 ggttatgttc aagaaagaac tatttttttc aaagatgacg gtaactacaa gaccagagct
      601 gaagtcaagt ttgaaggtga taccttagtt aatagaatcg aattaaaagg tattgatttt
      661 aaagaagatg gtaacatttt aggtcacaaa ttggaataca actataactc tcacaatgtt
      721 tacatcatgg ctgacaaaca aaagaatggt atcaaagtta acttcaaaat tagacacaac
      781 attgaagatg gttctgttca attagctgac cattatcaac aaaatactcc aattggtgat
      841 ggtccagtct tgttaccaga caaccattac ttatccactc aatctgcctt atccaaagat
      901 ccaaacgaaa agagagacca catggtcttg ttagaatttg ttactgctgc tggtattacc
      961 catggtatgg atgaattgta caaataaatc gcacgtattc cgttggtaga tacgttgttg
     1021 acacttctaa ataagcgaat ttcttatgat ttatgatttt tattattaaa taagttataa
     1081 aaaaaataag tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttat
     1141 tcttgagtaa ctctttcctg taggtcaggt tgctttctca ggtatagcat gaggtcgctc
     1201 atcgcacgca ttccatgcga gctcgctgag gacttaataa tcagacgcac gcttggcgtc
     1261 aatatcctaa aacggaaaac taatcagtta gatgtgtctt ttttaggtaa aaaaaggcaa
     1321 ccaatttttt gctggctagc gatgcgacaa taaacgcgcg cagcgaataa aaataagatg
     1381 gcggaggagc agagacttct tttcttagaa gaggttttgt taccgtatgg gggtatgatg
     1441 gttagatggc tgaataaaaa cgttaattaa aggaatacag aagaggaagc gccccgattt
     1501 cttttctgtt ccacatgaaa gcagccggtt gggtggtaga tctctaatga catccaaact
     1561 acaaaaccga gattggacat atagcacact ctgcatttct actttccttt ctccctacag
     1621 tttgctcgat ctgaaccgat gcttctttga ttggacaaga tgatagacca tgactccctg
     1681 tatgtattgc agagattgcc actactaacc aagaaaagtt gaacgtgtcg tacgtataaa
     1741 acgatttatg cttaggcttt actgaaaaaa aaaaagtaaa aagaggatgc gcaaacgcat
     1801 agagcaagta actattaaac cctcagcgcg gccgcattta aatccccact tcagaagttc
     1861 ctatactttc tagagaatag gaacttcact atagagtcga ataagggcga caccccataa
     1921 ttagcccggg cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcctggc
     1981 agttccctac tctcgcatgg ggagtcccca cactaccatc ggcgctacgg cgtttcactt
     2041 ctgagttcgg catggggtca ggtgggacca ccgcgctact gccgccaggc aaacaagggg
     2101 tgttatgagc catattcagg tataaatggg ctcgcgataa tgttcagaat tggttaattg
     2161 gttgtaacac tgacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     2221 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagaa tatgagtatt
     2281 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     2341 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     2401 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     2461 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     2521 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     2581 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     2641 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     2701 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     2761 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagcg
     2821 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     2881 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     2941 ccggctggct ggtttattgc tgataaatcc ggagccggtg agcgtggttc tcgcggtatc
     3001 atcgcagcgc tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     3061 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     3121 aagcattggt aagcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa
     3181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     3241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     3421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     3661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     3901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     3961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     4021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     4081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     4141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     4201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     4261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     4321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     4381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     4441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     4501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatgcattta
     4561 gaataaattt tgtgtcgccc ttattcgact cactatagaa gttcctattc tctagaaagt
     4621 ataggaactt cacttcattt tatttaaatt tgcggccgcg ctgaggtctt aattgagaat
     4681 ccggaccagc agataatgca tgcagtggtg ttttgatgta taagcttgac atttttttgc
     4741 ttttcccgtt atgagaaatg ttttttttag actgaaaata aaagaatgga tagtattaaa
     4801 atagtaatga acgtaataag gataggacag gagcgatagg aaaagataga attttggagc
     4861 tttttttccc tctttttttg agcaggagcc aatagtagtc ggaaagatga gtgcatagat
     4921 ttttcgtatt ttatttcgag taaaaatatc acgctcttag gttcaataaa agaatatact
     4981 atttcttcaa aatgcttctc taatctattt ttttattttc cttaataact gaaatattat
     5041 tttttgatgt aaaatgagtg aacaagaata agggattttt ggtatgtttg caagttctgt
     5101 ggagttccat gtataaggta tacatattag aaaagcttta cgattatata ctaacattat
     5161 gtacaaaaca gtttaataat gatctgtatt gctggctcaa tccacgtaaa ttaatgcctc
     5221 agcactagtc ctgcaggggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     5281 gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtgcata ggccactagt
     5341 ggatctgata tcacctaata acttcgtata gcatacatta tacgaagtta tattaagggt
     5401 tctcgagagc tcgttttatt taggttctat cgaggagaaa aagcgacaag aagagataga
     5461 ccatggataa atgattatgt tctaaacact cctcagaagc tcatcgaact gtcatcctgc
     5521 gtgaagatta aaatccaact tagaaatttc gagcttacgg agacaatcat atgggagaag
     5581 caattggaag atagaaaaaa ggtactcggt acataaatat atgtgattct gggtagaaga
     5641 tcggtctgca ttggatggtg gtaacgcatt tttttacaca cattacttgc ctcgagcatc
     5701 aaatggtggt tattcgtgga tctatatcac gtgatttgct taagaattgt cgttcatggt
     5761 gacactttta gctttgacat gattaagctc atctcaattg atgttatcta aagtcatttc
     5821 aactatctaa gatgtggttg tgattgggcc attttgtgaa agccagtacg ccagcgtcaa
     5881 tacactcccg tcaattagtt gcaccatgtc cacaaaatca tataccagta gagctgagac
     5941 tcatgcaagt ccggttgcat cgaaactttt acgtttaatg gatgaaaaga agaccaattt
     6001 gtgtgcttct cttgacgttc gttcgactga tgagctattg aaacttgttg aaacgttggg
     6061 tccatacatt tgccttttga aaacacacgt tgatatcttg gatgatttca gttatgaggg
     6121 tactgtcgtt ccattgaaag cattggcaga gaaatacaag ttcttgatat ttgaggacag
     6181 aaaattcgcc gatatcggta acacagtcaa attacaatat acatcgggcg tttaccgtat
     6241 cgcagaatgg tctgatatca ccaacgccca cggggttact ggtgctggta ttgttgctgg
     6301 cttgaaacaa ggtgcgcaag aggtcaccaa agaaccaagg ggattattga tgcttgctga
     6361 attgtcttcc aagggttctc tagcacacgg tgaatatact aagggtaccg ttgatattgc
     6421 aaagagtgat aaagatttcg ttattgggtt cattgctcag aacgatatgg gaggaagaga
     6481 agaagggttt gattggctaa tcatgacccc aggtgtaggt ttagacgaca aaggcgatgc
     6541 attgggtcag cagtacagaa ccgtcgacga agttgtaagt ggtggatcag atatcatcat
     6601 tgttggcaga ggacttttcg ccaagggtag agatcctaag gttgaaggtg aaagatacag
     6661 aaatgctgga tgggaagcgt accaaaagag aatcagcgct ccccattaat tatacaggaa
     6721 acttaataga acaaatcaca tatttaatct aatagccacc tgcattggca cggtgcaaca
     6781 ctacttcaac ttcatcttac aaaaagatca cgtgatctgt tgtattggga tctctagacc
     6841 taataacttc gtatagcata cattatacga agttatatta agggttgtcg acctgcagcg
     6901 tacgaagctt cagctgacgc gatcttcgag cgtcccaaaa ccttctcaag caaggttttc
     6961 agtataatgt tacatgcgta cacgcgtctg tacagaaaaa aaagaaaaat ttgaaatata
     7021 aataacgttc ttaatactaa cataactata aaaaaataaa tagggaccta gacttcaggt
     7081 tgtctaactc cttccttttc ggttagagca gatgaataca cgcgatgcac acaccatagc
     7141 ttcaaaatgt ttctactcct tttttactct tccagatttt ctcggactcc gcgcatcgcc
     7201 gtaccacttc aaaacaccca agcacagcat actaaatttc ccctctttct tcctctaggg
     7261 tgtcgttaat tacccgtact aaaggtttgg aaaagaaaaa agagaccgcc t
//
