LOCUS       sequence-320175-        7329 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the DRT type 3 defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7329)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 7329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG011 (DRT type 3)
FEATURES             Location/Qualifiers
     source          1..7329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      5229..5276
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(5496..6155)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     6070..6089
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(6156..6258)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(6650..6667)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6784..7329)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(6864..6883)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcatc ttattaatca gataaaatat ttctagattt cagtgcaatt tatctcttca
       61 aatgtagcac ctgaagtcag ccccatacga tataagttgt aattctcatg ttagtcatgc
      121 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
      181 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgcatctca acttccccaa
      241 atgtccgtat tcatccataa ataccctgat ttataacaat tttaccgttt tttagtccat
      301 catcgtccgc agccatccag tagaatccga taaagaatgt gtataggatt gtgtatatgt
      361 tcctgttcgg tcatggattc ctatacacat gcctttaaac gatatgcaga ttcgccgcgc
      421 taagcctgaa gataaaccct atacgcttgg ggatgggcaa ggcttgtcat tgcttataga
      481 acctaatgga agcaagagct ggcggttccg ctaacgctaa gccggtaaac ccaagatgat
      541 ctcgcttggc gtttacccaa cgatcactct tgccgatgct cgttcccgtc gtgatgaagc
      601 tcgaaaactt gtggcagaag gaaagaaccc tagtgatgtt cgaaaagagc aaaagctggc
      661 tctgcaagca gagtcagaga acgccttcga aaagatagcc agagagtggc atcaacttaa
      721 atctgctaaa tggtcggcag gatatgcatc agacatcatg gaagcgttta agaacgacat
      781 ttttccttat gtgggaacaa ggcctgtgag tgagattaaa ccgctagagc tgctgaacgt
      841 actgcgtaaa attgagaaac gtggtgcgtt ggagaaaatg cggaaagtgc gtcagcgttg
      901 ctctgaagtg tttcgctacg caattgcaac gggtagagcg gagtacaatc ctgcggcaga
      961 tctttccagc gctctcgaag tgcaccaatc caatcatttc ccgttcctaa aagctgatga
     1021 gatacccgaa tttctgcgtg ccttagagag ttacaccggg agtaagcttg tccagatagc
     1081 aacgaaatta ctgatgatta cgggcgtgag aaccatcgaa ttacgcgcgg cattatggca
     1141 agaatttgat ctggataacg ctatttggga aattcctgct gaaaggatga aaatgcgcag
     1201 gccgcatctt gtgccattgt cgacccaagc gttagattta ctccatgaac tcaagataat
     1261 gacagggaac tatcgttatg tttttccagg acggaacgat ccgaacaaac cgatgagcga
     1321 agctagcata aatcaagtta tcaagcgtat cggttacgaa ggccgactca ctggtcacgg
     1381 gttcagacat atgttatcaa caattttgca tgaagaaggt tttcaatcag catttattga
     1441 agtccaatta gctcatgttg atagaaataa tataagagga acttataatc atgccatata
     1501 ccttatggaa aggcagaaga tgatgcaatg gtacagtgat tatcttcgca aaaaaaaggg
     1561 gttataatat gttaaaccag tcattttccg tttcgaactt aattaagctt ttaaaaaaaa
     1621 ccgatccaaa aagatacaaa attggtagga attcagctga atataaaaaa tatatagctg
     1681 ataaagttaa tggctcaatt gaaacatact catttggttc gatctcaaat tcaagaatta
     1741 acaacaaaaa tgtgtatata tttaaagatt ttatggatgt acttgtcgcc aggaaaataa
     1801 atgataacat taagcgtgtg tatagtgtta aacaaaacaa cagacatgac atcataaaaa
     1861 aagtaaatac agtgttaagt gagcctgtaa attattatat ttacaggctg gatattaaga
     1921 gtttttatga atcaatagat aaaaatatcg ttttccaaag aattaataat aacccgatta
     1981 tttctcataa tactaaaaaa tttatcaatg gtctttttaa acataacgct ttctctgcaa
     2041 ataacggact tccccgtggt atgggattaa gtgcgacttt atcagaaata tttatggagg
     2101 aatttgatgc tgagttggcg aggctgcctg aagtatttta tgcttcaaga tatgtggatg
     2161 atatcatagt tttttcattc tataaaatac cagattataa aaattatttt tcaaggattt
     2221 taccaaatgg attacattta aatgaaagaa agtgcagtga gtataccata gaggacactt
     2281 caactaaaca ttctgaaatt gagtttttgg gatattcatt tattatacac catggattaa
     2341 aaaatcagcg tcgtcatgtt gtgatcagaa tttcggagga gaaaataaag aaaataaaaa
     2401 gaaggattgc acttgcggta aaagattact caaataattc tgatgcagaa ctcttgaaga
     2461 aaagaataaa gtatttaact ggtaatatat tagtaaactc caatagtaat aaaactgatg
     2521 ctttatatag tggaatttat tacaattatc aacatttaac tgataaaaca cagctcaagg
     2581 aacttgatat atttaagaat aggatgctat tttcttcaaa gggcgaggtg gggagaaaaa
     2641 ttttagcagc aggtcacaac ttattaactg cgcctaaaaa atactcattt ttggctggtt
     2701 ttgaaaaacg gctactgtct tcttttaaac gggaagatat tattaaaata aataaggttt
     2761 ggtgattcat gaaaattaaa atatcgaaga gtgattataa aagagtactt ctcacggata
     2821 ttttaccata tgaagtccct atcctttttt ctaacgaagg tttctataag ttaatttctg
     2881 aaaataaagt tttacccgga acattttcag aaggccttaa gctggattct tataccatcc
     2941 cttactccta taaaataaaa aaggggctgg cgagttctcg aagccttggc attatacatc
     3001 cttcaacgca gttaagaatc tgtgattttt atgataagta tgaacatttg atggttcata
     3061 tgtgtacaaa aagtccgttt tcgctacgtt atcctagcaa aatagggagc tattattacg
     3121 aaaaggactt cttaaaaagt agaataaatc taaaagatgg tcttgtacaa tttcataatc
     3181 atggctttga ttcccaagaa acttcctcat cttcccattt ttcatataag aaatatcctt
     3241 tcatctataa gttttatgag tcatatgaat ttcatagatt ggaaaggaag tttaggaaac
     3301 ttttaaagct tgatattgct aagtgtttta gtcatatata tacacacagc gtttcatggg
     3361 ctgtaaaatc taaagaattc tctaaggtta atagaactta taacagcttt gaaggttgtt
     3421 tggataagct ttttcaagat gccaattatg gtgaaacaaa tggcataata attgggcctg
     3481 aattttcaag gatatttgcg gagattatat tacagcgcgt tgacttgaat gttgagtctc
     3541 atttgaatct tgagccaggc atagttaaag ataagagcta tgctataaga cgttacgttg
     3601 atgattattt tatatttgcg gatgatgatg aaacatttaa gctaatagaa tttgtactgg
     3661 caaatgaact cgaaaaatat aagctttatt tgaatgaatc taaaaaggaa tttatcgaga
     3721 ggccattcgt gactggagct acgatggcta aaaatgatat tgcagaaatc attgaggatt
     3781 tatatggatc gttaatccat actgagaagt tggatgagtt aacagctatg gttaatttaa
     3841 atccagacgt caaaattcag cctgaaaata tgaatgacct ttttccattg aaaggtgtgt
     3901 ggaataaaaa gctacacgcg gacaaattta taaaacgaat caaaattgcg gttagaaaaa
     3961 acaataccac atttgatctt gttagctcat acttattaag tgcgattaag agtaagtttt
     4021 tcaaagtaat taggctgttg aggatgttcg atctgtcagg aaaagaagat ataacttata
     4081 aattcttctc aatattcaat gaggtgattt tttttattta tgctatggat tttcgagtcc
     4141 gacagacata cataattagc caagttattt tggaaataaa ttcatttgct aataagcaag
     4201 cttcagacat tagtgaagtt ataaaaaaga atacttttga tgagcttctt atgtgcatga
     4261 aaagcatggg taatattcat gagaggccag tggagttatc taacttactt atatgtatga
     4321 aaggtttggg ggagcagtat aaactcaatc cagatgaatt taaggatttg ttgggtatta
     4381 gtgagaatga gtgtttttac gatttagaat atttttctat atgcagcatg ttacactata
     4441 taggcgatga tgttctctat ctaaaaatga aagaagatat tgtccttgct atacagagtt
     4501 tgataagtgg tcggaacgat ataaaaaaag acactgaaac atttatgcta ttccttgata
     4561 tgatgacgtg cccatatctt acagttaagc ataagagaat aatttataga acatatgtcg
     4621 aagcaaatac aggtcaaaaa agatttacga atgcagtaat tgattctgaa attgattctt
     4681 taaaaaataa tgtaatcttt tttaactggt ctggagatgc tgatcttgag cacgttcttt
     4741 ataaaaaaga gttgcgaaca gcatatgaat agtagtattt taatttcgtt aaagggttgc
     4801 gatgcctaag gtttcgacct gaagcagata ccggaagatc ggcttttgaa tgttcatccg
     4861 aaagatattc gcgatacgtt ttgaggatgg accgatttag acacactatt gccttttagc
     4921 taaacaggcc gcgaaagcgg cctttttaat gaatcagatt tcccctcacc gatctcaata
     4981 cttcccctca gcgtgcgcag ccccgcccgc ctgcccgctt cgcttaacag actggttttc
     5041 atgcacccct taaatcgtct cagaagccac cacacaaggg ctttcgcgtc aaaaatggcg
     5101 catgagactc atgcgttttc atgcgccata gatatgcact catacgctct caggccagct
     5161 agggaaaaag cgtaaaaaat cccggtactg gaccgagact tcgtgggcgt attttgctaa
     5221 cgcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
     5281 aacctcaggc atttgagaag cacacggtca cactgcttcc ggtagtcaat aaaccggtaa
     5341 accagcaata gacataagcg gctatttaac gaccctgccc tgaaccgacg accgggtcga
     5401 atttgctttc gaatttctgc cattcatccg cttattatca cttattcagg cgtagcacca
     5461 ggcgtttaag ggcaccaata actgccttaa aaaaattacg ccccgccctg ccactcatcg
     5521 caatactgtt gtaattcatt taacattctg ccgacatgga agccatcaca gacggcatga
     5581 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccata
     5641 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
     5701 ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag
     5761 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
     5821 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
     5881 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcatcgc catacggaac
     5941 tctggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
     6001 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
     6061 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
     6121 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
     6181 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     6241 cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg cttcccggta
     6301 tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac aggtatttat
     6361 tcggcgcaaa gtgcgtcggg tgatgctgcc aacttactga tttagtgtat gatggtgttt
     6421 ttgaggtgct ccagtggctt ctgtttctat cagctgtccc tcctgttcag ctactgacgg
     6481 ggtggtgcgt aacggcaaaa gcaccgccgg acatcagcgc tagcggagtg tatactggct
     6541 tactatgttg gcactgatga gggtgtcagt gaagtgcttc atgtggcagg agaaaaaagg
     6601 ctgcaccggt gcgtcagcag aatatgtgat acaggatata ttccgcttcc tcgctcactg
     6661 actcgctacg ctcggtcgtt cgactgcggc gagcggaaat ggcttacgaa cggggcggag
     6721 atttcctgga agatgccagg aagatactta acagggaagt gagagggccg cggcaaagcc
     6781 gtttttccat aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg
     6841 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggcg gctccctcgt
     6901 gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt
     6961 gtctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta
     7021 tgcacgaacc ccccgttcag tccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7081 ccaacccgga aagacatgca aaagcaccac tggcagcagc cactggtaat tgatttagag
     7141 gagttagtct tgaagtcatg cgccggttaa ggctaaactg aaaggacaag ttttggtgac
     7201 tgcgctcctc caagccagtt acctcggttc aaagagttgg tagctcagag aaccttcgaa
     7261 aaaccgccct gcaaggcggt tttttcgttt tcagagcaag agattacgcg cagaccaaaa
     7321 cgatctcaa
//
