LOCUS       sequence-320199-       13569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses four gRNA targeting Mhc gene with 3xp3-tdTomato marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13569)
  AUTHORS   Li M, Yang T, Bui M, Gamez S, Wise T, Kandul NP, Liu J, Alcantara L,
            Lee H, Edula JR, Raban R, Zhan Y, Wang Y, DeBeaubien N, Chen J, 
            Sanchez C HM, Bennett JB, Antoshechkin I, Montell C, Marshall JM, 
            Akbari OS
  TITLE     Suppressing mosquito populations with precision guided sterile 
            males.
  JOURNAL   Nat Commun. 2021 Sep 10;12(1):5374. doi: 10.1038/s41467-021-25421-w.
  PUBMED    34508072
REFERENCE   2  (bases 1 to 13569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-09-10"; volume: "12(1):5374. doi"; pages: " 
            10.1038/s41467-021-25421-w"
COMMENT     Sequence Label: OA-1067K
FEATURES             Location/Qualifiers
     source          1..13569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(95..683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(175..194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(854..1645,1646..1714))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1477..1496
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1715..1819)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1887..1905
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1943..1965)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2065..2084
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2278..2300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2292..2309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2293..2309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   2998..3060
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     regulatory      7528..7537
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7667..7685)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7935..7954
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8129..8152
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    9086..9207
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9123..9142)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9177..9196
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   13046..13080
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(13262..13278)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13262..13278)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13275..13297)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13286..13302
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13310..13316,13317..13334,13335..13340))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13355..13376
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13493..13510)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1861 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     1921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2041 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2101 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2161 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2221 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2281 agtcacgacg ttgtaaaacg acggccagtg ccaagctttg tttaaaatat aacaaaattg
     2341 tgatcccaca aaatgaagtg gggcaaaatc aaataattaa ctagtgtccg taaacttgtt
     2401 ggtcttcaac tttttgagga acacgttgga cggcaaatcg tgactataac acaagttgat
     2461 ttaataattt tagccaacac gtcgggctgc gtgttttttg cgctctgtgt acacgttgat
     2521 taactggtcg attaaataat ttaatttttg gttcttcttt aaatctgtga tgaaattttt
     2581 taaaataact ttaaattctt cattggtaaa aaatgccacg ttttgcaact tgtgagggtc
     2641 taatatgagg tcaaactcag taggagtttt atccaaaaaa gaaaacatga ttacgtctgt
     2701 acacgaacgc gtattaacgc agagtgcaaa gtataagagg gttaaaaaat atattttacg
     2761 caccatatac gcatcgggtt gatatcgtta atatggatca atttgaacag ttgattaacg
     2821 tgtctctgct caagtctttg atcaaaacgc aaatcgacga aaatgtgtcg gacaatatca
     2881 agtcgatgag cgaaaaacta aaaaggctag aatacgacaa tctcacagac agcgttgaga
     2941 tatacggtat tcacgacagc aggctgaata ataaaaaaat tagaaactat tatttaaccc
     3001 tagaaagata atcatattgt gacgtacgtt aaagataatc atgcgtaaaa ttgacgcatg
     3061 tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat
     3121 tatatttaca cttacatact aataataaat tcaacaaaca atttatttat gtttatttat
     3181 ttattaaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ttttaaacat
     3241 tctctctttt acaaaaataa acttattttg tactttaaaa acagtcatgt tgtattataa
     3301 aataagtaat tagcttaact tatacataat agaaacaaat tatacttatt agtcagtcag
     3361 aaacaacttt ggcacatatc aatattatgc tctcgacaaa taactttttt gcattttttg
     3421 cacgatgcat ttgcctttcg ccttatttta gaggggcagt aagtacagta agtacgtttt
     3481 ttcattactg gctcttcagt actgtcatct gatgtaccag gcacttcatt tggcaaaata
     3541 ttagagatat tatcgcgcaa atatctcttc aaagtaggag cttctaaacg cttacgcata
     3601 aacgatgacg tcaggctcat gtaaaggttt ctcataaatt ttttgcgact ttgaaccttt
     3661 tctcccttgc tactgacatt atggctgtat ataataaaag aatttatgca ggcaatgttt
     3721 atcattccgt acaataatgc cataggccac ctattcgtct tcctactgca ggtcatcaca
     3781 gaacacattt ggtctagcgt gtccactccg cctttagttt gattataata cataaccatt
     3841 tgcggtttac cggtactttc gttgatagaa gcatcctcat cacaagatga taataagtat
     3901 accatcttag ctggcttcgg tttatatgag acgagagtaa ggggtccgtc aaaacaaaac
     3961 atcgatgttc ccactggcct ggagcgactg tttttcagta cttccggtat ctcgcgtttg
     4021 tttgatcgca cggttcccac aatggttaat tcgagctcgc ccgggctcct aggagatctt
     4081 catcacaaac gaaactatta taatcgtctt caggggaacg gtagcatcat atctgtcacc
     4141 agtcagtcgg attcctttct ttcggcataa attatgctcg ggtgacgaga cgagttgaaa
     4201 atcccgtaaa ttaaaacata taccacactc gccgtcctag acgacgacga cgacgacgat
     4261 tcccaaggaa ttgaaccctt catttgcatt tcactaccgc tctgagagat gcaaagcgcg
     4321 ccagacagtc gatcgaatct gcagaccaga gagtcacatc aagaatgttg acattccacc
     4381 agaagactct aattattctt cgctcgactg ggaaaactgt taacaactgc tcgagtgtgg
     4441 tcggagcggc tcacagtgcg ttttcccgta gacaaaaatc aaatttcatg gtatttttcg
     4501 gcgataaaca gtaagttaaa gggaacccga atttagtgct atataattta attccactag
     4561 agtttgtatc ctttgataga tacgcgtatt tcgacctcaa ctgcaaggcc gtcgtgtact
     4621 agacttgact aatccagact ggtcttttag ttatgacttc tgtccacatc tccatacatt
     4681 caacgcactg tgcggctgtg ctgtgcgact ccgtcgagtc gaccaacata gttgaaacaa
     4741 attgaatatt taattgatcg ttataggaat ggtgttagat gagtcatcct ttacagtaag
     4801 cacatacagt attataattg aagatcgtcg gcagataggt gtgtagggta gagtatcagc
     4861 aataagttgg gacgtttgac tttttgtagg tagacaaaaa ctaaactttt tttcgcttct
     4921 ctatgtgtgc ccctgggtag cgttccgttc cgattggggt gcgaacgaat gaaatcgccc
     4981 atcgagttga tacgtccatc catcgctaga accgcgttcg ctgtagaaga ctatataaga
     5041 gcagaggcaa gagtagtgaa atgaaatgaa gagccattcg gagtcttaga gctatgctgg
     5101 aaacagcata gcaagttaag ataaggctag tccgttatca acttgaaaaa gtggcaccga
     5161 gtcggtgctt tttttggtgg ttgttcatcg attcctaata ttgcagtgaa gcagtttgtt
     5221 gtgcatggcg aagataaggt tagggcgagg aatgccttgg aaataagcgg cactgggccg
     5281 tatgaataaa aagcgttgaa ctttactaca tgtagcatct actcaaaaac aaaatccaca
     5341 aagtttacta cacttttggt atgaataaaa accttttcga acatgtagta cctactactt
     5401 taagaaaatt ctattcaaag tacagagtaa tgctgcatgt taagaaaatt ccattcagag
     5461 tgactcttga aatcaataaa atcatgcata tatgccaaat ttgtcactta ttcacgtgac
     5521 aaattatgtg aaaatatgaa tattactaca ttcccgctat gcatcgcgta atcgatcatg
     5581 aaatttatgt tggttatgta actttacagt attcccttta tgtgcatgtg aattttataa
     5641 tgatttgaca acaaggattt tccttagaat tttcgaattt tagctagaaa tctgtatcct
     5701 atgaatgaat cttgagataa cagcaattgt aacattataa agttcctaac attttcaaga
     5761 ttccagtatt gagtcgcgat cgcttaatta accgcggata tgtcttgccg aagcacatta
     5821 acccaagcca tttatatatc aagtacaagt tgatttgatt ctagtgatgg actgacacga
     5881 atctatggca ctgttgcaga gcttacaaac aggtacccgg agtcgcttca attattcaat
     5941 catccattca aaaacttcca tctcttccaa ttagccaata ttcaaattgg aatccagcca
     6001 gccgacggag gcagatggtc tacctggcaa ctatagagtt tccatgtttc cagactttcc
     6061 ctcccggtaa acggagacaa aacgacagac gtaagtaggt acatatgcat accgcacgga
     6121 caaatcaaat ttgtctggca gctccaatta gagtcgttaa aaatttaacg atgcgctaaa
     6181 taacttcaag ctatttgtct cgctggattg gcttcgagtg gtaagatcct atcaaatgcc
     6241 gaaaacaaaa aactttcttc ttaattgttc gttcttcaac acctctccat ggtgataacg
     6301 gatacggttt cattgtcagc atccatcctc cgaaaaatac attacgcctt gaaatatgca
     6361 atcgcaaaca cggatctgtt tggaacattt attttactat gaagagatgc gataggtaat
     6421 atttatttga gcgtttaaga tactcattgt tctctcaaag aatgtcattg aaagccaacg
     6481 aggtcaaatc aaatattata ataaaaaggt caaagaggac taacttaaag ctctctttat
     6541 ggataggaaa aaatattttc gcccatcgct agaactttta ccgtttccat tgagtatata
     6601 actaagatga atgaggctaa ttgatggtga aatcaaagca accaagtctt agagctatgc
     6661 tggaaacagc atagcaagtt aagataaggc tagtccgtta tcaacttgaa aaagtggcac
     6721 cgagtcggtg ctttttttaa gatgcttcgg caattaataa taaggaatgg taaagcattc
     6781 ttcaaatgtt actatgttct caaattttca agaattaggt attcaagaaa ttctttcagg
     6841 catattccat aatctgcaca agcagctact ttagaaatta ttgccgggct taaattatat
     6901 gtacaacatt tcctgagtat tttctgacaa aaattctcac aagggaaggt cacgatggag
     6961 ttacacgggg tttttaaccg gactattttt gttagattct acatgtcgaa atatgaaaaa
     7021 ccccaaagcc tttcgggtga tggaccagtg gtgtagccag gaggggctct gaaaggggta
     7081 attttgtacg tatattatat tattaagcta attggaaatt tgacaaaaat atttttttag
     7141 tatttattgt gatggtagag aacaaaattg acattaatgt acgtttgaaa tgtatttgcc
     7201 atgcctttgg cgtactaaca tggagtatgg acaccaaaac cgatttcggc cttttgggcc
     7261 cggcgcgcca attcgagctc gcccggggat ctaattcaat tagagactaa ttcaattaga
     7321 gctaattcaa ttaggatcca agcttatcga tttcgaaccc tcgaccgccg gagtataaat
     7381 agaggcgctt cgtctacgga gcgacaattc aattcaaaca agcaaagtga acacgtcgct
     7441 aagcgaaagc taagcaaata aacaagcgca gctgaacaag ctaaacaatc ggggtaccgc
     7501 tagagtcgac ggtacgatcc accggtcgcc accatggtga gcaagggcga ggaggtcatc
     7561 aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca cgagttcgag
     7621 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     7681 accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt catgtacggc
     7741 tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct gtccttcccc
     7801 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc
     7861 caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg cggcaccaac
     7921 ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag
     7981 cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct gaagctgaag
     8041 gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa gcccgtgcaa
     8101 ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa cgaggactac
     8161 accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct ggggcatggc
     8221 accggcagca ccggcagcgg cagctccggc accgcctcct ccgaggacaa caacatggcc
     8281 gttatcaagg aatttatgcg cttcaaagtt aggatggagg gatccatgaa cggacatgag
     8341 ttcgagatcg agggagaggg cgagggacgc ccgtatgaag gcacacaaac agccaaactc
     8401 aaggtcacca agggcggacc actgcccttc gcctgggata tcctgagtcc ccagtttatg
     8461 tacggcagca aggcctacgt taagcacccc gctgacatac cggactacaa aaagctgtcc
     8521 tttccggaag gcttcaagtg ggagcgcgtg atgaatttcg aagacggagg actggtcact
     8581 gtgacccaag atagcagttt gcaggacggt acactgatct ataaggttaa aatgcgcggc
     8641 actaactttc cgccagatgg cccagtgatg cagaagaaga ccatgggttg ggaggcatcc
     8701 accgaacgtc tgtaccctcg agacggagtg ctcaagggcg agatccatca ggccctcaaa
     8761 ctgaaagatg gtggtcacta cctggtcgaa tttaagacca tttacatggc caagaagccg
     8821 gttcagctgc ccggatatta ttatgtggat acgaaactgg atataacttc gcataacgaa
     8881 gactacacca ttgtcgagca gtatgagcgc agcgaaggcc gacatcacct gttcctctac
     8941 ggcatggacg agctgtacaa gtaggcggcc gcgactctag atcataatca gccataccac
     9001 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     9061 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     9121 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     9181 tttgtccaaa ctcatcaatg tatcttaaag cttgctagct catcacaaac gaaactatta
     9241 taatcgtctt caggggaacg gtagcatcat atctgtcacc agtcagtcgg attcctttct
     9301 ttcggcataa attatgctcg ggtgacgaga cgagttgaaa atcccgtaaa ttaaaacata
     9361 taccacactc gccgtcctag acgacgacga cgacgacgat tcccaaggaa ttgaaccctt
     9421 catttgcatt tcactaccgc tctgagagat gcaaagcgcg ccagacagtc gatcgaatct
     9481 gcagaccaga gagtcacatc aagaatgttg acattccacc agaagactct aattattctt
     9541 cgctcgactg ggaaaactgt taacaactgc tcgagtgtgg tcggagcggc tcacagtgcg
     9601 ttttcccgta gacaaaaatc aaatttcatg gtatttttcg gcgataaaca gtaagttaaa
     9661 gggaacccga atttagtgct atataattta attccactag agtttgtatc ctttgataga
     9721 tacgcgtatt tcgacctcaa ctgcaaggcc gtcgtgtact agacttgact aatccagact
     9781 ggtcttttag ttatgacttc tgtccacatc tccatacatt caacgcactg tgcggctgtg
     9841 ctgtgcgact ccgtcgagtc gaccaacata gttgaaacaa attgaatatt taattgatcg
     9901 ttataggaat ggtgttagat gagtcatcct ttacagtaag cacatacagt attataattg
     9961 aagatcgtcg gcagataggt gtgtagggta gagtatcagc aataagttgg gacgtttgac
    10021 tttttgtagg tagacaaaaa ctaaactttt tttcgcttct ctatgtgtgc ccctgggtag
    10081 cgttccgttc cgattggggt gcgaacgaat gaaatcgccc atcgagttga tacgtccatc
    10141 catcgctaga accgcgttcg ctgtagaaga ctatataaga gcagaggcaa gagtagtgaa
    10201 atggcttcat ctggaaccca gcgtcttaga gctatgctgg aaacagcata gcaagttaag
    10261 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt tttttggtgg
    10321 ttgttcatcg attcctaata ttgcagtgaa gcagtttgtt gtgcatggcg aagataaggt
    10381 tagggcgagg aatgccttgg aaataagcgg cactgggccg tatgaataaa aagcgttgaa
    10441 ctttactaca tgtagcatct actcaaaaac aaaatccaca aagtttacta cacttttggt
    10501 atgaataaaa accttttcga acatgtagta cctactactt taagaaaatt ctattcaaag
    10561 tacagagtaa tgctgcatgt taagaaaatt ccattcagag tgactcttga aatcaataaa
    10621 atcatgcata tatgccaaat ttgtcactta ttcacgtgac aaattatgtg aaaatatgaa
    10681 tattactaca ttcccgctat gcatcgcgta atcgatcatg aaatttatgt tggttatgta
    10741 actttacagt attcccttta tgtgcatgtg aattttataa tgatttgaca acaaggattt
    10801 tccttagaat tttcgaattt tagctagaaa tctgtatcct atgaatgaat cttgagataa
    10861 cagcaattgt aacattataa agttcctaac attttcaaga ttccagtatt gagtcgcgat
    10921 cgcttaatta accgcggata tgtcttgccg aagcacatta acccaagcca tttatatatc
    10981 aagtacaagt tgatttgatt ctagtgatgg actgacacga atctatggca ctgttgcaga
    11041 gcttacaaac aggtacccgg agtcgcttca attattcaat catccattca aaaacttcca
    11101 tctcttccaa ttagccaata ttcaaattgg aatccagcca gccgacggag gcagatggtc
    11161 tacctggcaa ctatagagtt tccatgtttc cagactttcc ctcccggtaa acggagacaa
    11221 aacgacagac gtaagtaggt acatatgcat accgcacgga caaatcaaat ttgtctggca
    11281 gctccaatta gagtcgttaa aaatttaacg atgcgctaaa taacttcaag ctatttgtct
    11341 cgctggattg gcttcgagtg gtaagatcct atcaaatgcc gaaaacaaaa aactttcttc
    11401 ttaattgttc gttcttcaac acctctccat ggtgataacg gatacggttt cattgtcagc
    11461 atccatcctc cgaaaaatac attacgcctt gaaatatgca atcgcaaaca cggatctgtt
    11521 tggaacattt attttactat gaagagatgc gataggtaat atttatttga gcgtttaaga
    11581 tactcattgt tctctcaaag aatgtcattg aaagccaacg aggtcaaatc aaatattata
    11641 ataaaaaggt caaagaggac taacttaaag ctctctttat ggataggaaa aaatattttc
    11701 gcccatcgct agaactttta ccgtttccat tgagtatata actaagatga atgaggctaa
    11761 ttgatgtaac cgttgcccta cccgggtctt agagctatgc tggaaacagc atagcaagtt
    11821 aagataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttaa
    11881 gatgcttcgg caattaataa taaggaatgg taaagcattc ttcaaatgtt actatgttct
    11941 caaattttca agaattaggt attcaagaaa ttctttcagg catattccat aatctgcaca
    12001 agcagctact ttagaaatta ttgccgggct taaattatat gtacaacatt tcctgagtat
    12061 tttctgacaa aaattctcac aagggaaggt cacgatggag ttacacgggg tttttaaccg
    12121 gactattttt gttagattct acatgtcgaa atatgaaaaa ccccaaagcc tttcgggtga
    12181 tggaccagtg gtgtagccag gaggggctct gaaaggggta attttgtacg tatattatat
    12241 tattaagcta attggaaatt tgacaaaaat atttttttag tatttattgt gatggtagag
    12301 aacaaaattg acattaatgt acgtttgaaa tgtatttgcc atgcctttgg cgtactaaca
    12361 tggagtatgg acaccaaaac cgatttcgtt taaacgatct cggatctgac aatgttcagt
    12421 gcagagactc ggctacgcct cgtggacttt gaagttgacc aacaatgttt attcttacct
    12481 ctaatagtcc tctgtggcaa ggtcaagatt ctgttagaag ccaatgaaga acctggttgt
    12541 tcaataacat tttgttcgtc taatatttca ctaccgcttg acgttggctg cacttcatgt
    12601 acctcatcta taaacgcttc ttctgtatcg ctctggacgt catcttcact tacgtgatct
    12661 gatatttcac tgtcagaatc ctcaccaaca agctcgtcat cgctttgcag aagagcagag
    12721 aggatatgct catcgtctaa agaactaccc attttattat atattagtca cgatatctat
    12781 aacaagaaaa tatatatata ataagttatc acgtaagtag aacatgaaat aacaatataa
    12841 ttatcgtatg agttaaatct taaaagtcac gtaaaagata atcatgcgtc attttgactc
    12901 acgcggtcgt tatagttcaa aatcagtgac acttaccgca ttgacaagca cgcctcacgg
    12961 gagctccaag cggcgactga gatgtcctaa atgcacagcg acggattcgc gctatttaga
    13021 aagagagagc aatatttcaa gaatgcatgc gtcaatttta cgcagactat ctttctaggg
    13081 ttaaaaaaga tttgcgcttt actcgaccta aactttaaac acgtcataga atcttcgttt
    13141 gacaaaaacc acattgtggc caagctgtgt gacgcgacgc gcgctaaaga atggcaaacc
    13201 aagtcgcgcg agcgtcgact ctagaggatc cccgggtacc gagctcgaat tcgtaatcat
    13261 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
    13321 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
    13381 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
    13441 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
    13501 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    13561 taatacggt
//
