LOCUS       sequence-32042-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Kwon HJ, Abi-Mosleh L, Wang ML, Deisenhofer J, Goldstein JL, Brown 
            MS, Infante RE
  TITLE     Structure of N-terminal domain of NPC1 reveals distinct subdomains 
            for binding and transfer of cholesterol.
  JOURNAL   Cell. 2009 Jun 26. 137(7):1213-24.
  PUBMED    19563754
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jun 26. 137(7):1213-24."
COMMENT     Sequence Label: pfast NPC1(NTD)
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     82..144
                     /codon_start=1
                     /product="signal sequence from honeybee melittin"
                     /label=melittin signal sequence
                     /translation="MKFLVNVALVFMVVYISYIYA"
     CDS             157..174
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             196..216
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 acgttttgta taaaaaaacc tataaatatt ccggattatt cataccgtcc caccatcggg
       61 cgcggatctc ggtccgaaac catgaaattc ttagtcaacg ttgcccttgt ttttatggtc
      121 gtatacattt cttacatcta tgcggattcg tactaccatc accatcacca tcacgattac
      181 gatatcccaa cgaccgaaaa cctgtatttt cagggcgccc agtcctgtgt ttggtatgga
      241 gagtgtggaa ttgcatatgg ggacaagagg tacaattgcg aatattctgg cccaccaaaa
      301 ccattgccaa aggatggata tgacttagtg caggaactct gtccaggatt cttctttggc
      361 aatgtcagtc tctgttgtga tgttcggcag cttcagacac taaaagacaa cctgcagctg
      421 cctctacagt ttctgtccag atgtccatcc tgtttttata acctactgaa cctgttttgt
      481 gagctgacat gtagccctcg acagagtcag tttttgaatg ttacagctac tgaagattat
      541 gttgatcctg ttacaaacca gacgaaaaca aatgtgaaag agttacaata ctacgtcgga
      601 cagagttttg ccaatgcaat gtacaatgcc tgccgggatg tggaggcccc ctcaagtaat
      661 gacaaggccc tgggactcct gtgtgggaag gacgctgacg cctgtaatgc caccaactgg
      721 attgaataca tgttcaataa ggacaatgga caggcacctt ttaccatcac tcctgtgttt
      781 tcagattttc cagtccatgg gatggagccc atgaacaatg ccaccaaagg ctgtgacgag
      841 tctgtggatg aggtcacagc accatgtagc tgccaagact gctctattgt ctgtgg
//
