LOCUS       sequence-32048-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Carlson HJ, Cotton DW, Campbell RE
  TITLE     Circularly permuted monomeric red fluorescent proteins with new 
            termini in the beta-sheet.
  JOURNAL   Protein Sci. 2010 Aug;19(8):1490-9. doi: 10.1002/pro.428.
  PUBMED    20521333
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro"; 
            journalName: "Protein Sci"; date: "2010-08"; volume: "19"; issue: 
            "8"; pages: "1490-9"
COMMENT     Sequence Label: cp196
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             65..82
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             86..118
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(122..130,131..145)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(459..477)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     727..746
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctctctactg tttctccata cccgtttttt gggctaacag gaggaattaa ccatgggggg
       61 ttctcatcat catcatcatc atggtatggc tagcatgact ggtggacagc aaatgggtcg
      121 ggatctgtac gacgatgacg ataaggatcc gagctcgagc atgtccagct ccggggcact
      181 gggcggtttt atcaagttgg acatcacctc acacaacgag gactacacca tcgtggaaca
      241 gtacgaacgc gccgaggtcc gccactccac cggcggcatg gacgagctgt acaagggtgg
      301 cactggcggt tccatggtga gcaagggcgt ggaggataac atggccttca tcaaggagtt
      361 catgcgcttc aaggtgcaca tggagggctc cgtcaacggc cacgagttcg agatcgaggg
      421 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
      481 tggccccctg cccttcgcct gggacatcct gtcccctcag ctcatgtacg gctccaaggc
      541 ctacgtgaag caccccgccg acatccccga ctactggaag ctgtccttcc ccgagggctt
      601 caagtgggag cgcgtgatga acttcgagga cggaggcgtg gtgaccgtga cccaggactc
      661 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
      721 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
      781 ccccgaggac ggcgctctga agggcgagat caagcagagg ctgaagctga aggacggcgg
      841 ccactacgac gctgaggtca agaccaccta caaggccaag aagcctgtgc agctg
//
