LOCUS       sequence-32050-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Carlson HJ, Cotton DW, Campbell RE
  TITLE     Circularly permuted monomeric red fluorescent proteins with new 
            termini in the beta-sheet.
  JOURNAL   Protein Sci. 2010 Aug;19(8):1490-9. doi: 10.1002/pro.428.
  PUBMED    20521333
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro"; 
            journalName: "Protein Sci"; date: "2010-08"; volume: "19"; issue: 
            "8"; pages: "1490-9"
COMMENT     Sequence Label: cp193g7N
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             76..93
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             97..129
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(133..141,142..156)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(455..473)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     723..742
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tttttatcgc actctctact gtttctccat acccgttttt tgggctaaca ggaggaatta
       61 accatggggg gttctcatca tcatcatcat catggtatgg ctagcatgac tggtggacag
      121 caaatgggtc gggatctgta cgacgatgac gataaggatc cgagctcgag ggcctacaac
      181 gtcgacatca agttggacat cacctcacac aacgaggact acaccatcgt ggaacagtac
      241 gaacgcgccg aggtccgcca ctccaccggc ggcatggacg agctgtacaa gggcggtacc
      301 ggcggctcca tggtgagcaa gggcgtggag gataacatgg ccttcatcaa ggagttcatg
      361 cgcttcaagg tgcacatgga gggctccgtc aacggccacg agttcgagat cgagggcgag
      421 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
      481 cccctgccct tcgcctggga catcctgtcc cctcagctca tgtacggctc caaggcctac
      541 gtgaagcacc ccgccgacat ccccgactac tggaagctgt ccttccccga gggcttcaag
      601 tgggagcgcg tgatgaactt cgaggacgga ggcgtggtga ccgtgaccca ggactcctcc
      661 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
      721 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
      781 gaggacggcg ctctgaaggg cgagatcaag caga
//
