LOCUS       sequence-320516-          20 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP sgRNA4 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 20)
  AUTHORS   Yuen G, Khan FJ, Gao S, Stommel JM, Batchelor E, Wu X, Luo J
  TITLE     CRISPR/Cas9-mediated gene knockout is insensitive to target copy 
            number but is dependent on guide RNA potency and Cas9/sgRNA 
            threshold expression level.
  JOURNAL   Nucleic Acids Res. 2017 Nov 16;45(20):12039-12053. doi: 
            10.1093/nar/gkx843.
  PUBMED    29036671
REFERENCE   2  (bases 1 to 20)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2017-11-16- 16"; volume: "45"; issue: 
            "20"; pages: "12039-12053"
COMMENT     Sequence Label: LentiCRISPRv2 EGFP sgRNA4
FEATURES             Location/Qualifiers
     source          1..20
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggagcgcacc atcttcttca
//
