LOCUS       sequence-32059-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Beronja S, Livshits G, Williams S, Fuchs E
  TITLE     Rapid functional dissection of genetic networks via tissue-specific 
            transduction and RNAi in mouse embryos.
  JOURNAL   Nat Med. 2010 Jul . 16(7):821-7.
  PUBMED    20526348
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Med. 
            2010 Jul . 16(7):821-7."
COMMENT     Sequence Label: LV-CFP
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             75..308
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(424..445)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     773..792
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gttcctctca ctctctgata ttcatttctt tgcaagttat aaatactgaa taataagatg
       61 acatgaacta ctactgctag agattttcca cactgactaa aagggtctga gggatctcta
      121 gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta
      181 ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc
      241 taaccagaga gacccagtac aagcaaaaag cagatcttgt cttcgttggg agtgaattag
      301 cccttccagt cccccctttt cttttaaaaa gtggctaaga tctacagctg ccttgtaagt
      361 cattggtctt aaaggtacct acttgtacag ctcgtccatg ccgagagtga tcccggcggc
      421 ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc
      481 ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt
      541 gttctgctgg tagtgntcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt
      601 gaagtgggcc ttgatgccgt tcttctgctt gtcggcggtg atatagacgt tgtggctgat
      661 gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt
      721 cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta
      781 gttgccgtcg tccttgaaga
//
