LOCUS       sequence-320714-        1677 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  protein expression in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Shen H, Forgacs D, Chapla D, Moremen KW, Wells L, Hamer SA, Tompkins
            SM, Ross TM, Rouphael N, Edupuganti S, Collins MH, Tarleton RL
  TITLE     A flexible, pan-species, multi-antigen platform for the detection 
            and monitoring of SARS-CoV-2-specific antibody responses.
  JOURNAL   medRxiv [Preprint]. 2021 Jan 27:2021.01.20.21249279. doi: 
            10.1101/2021.01.20.21249279.
  PUBMED    33532799
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv 
            [Preprint]. 2021 Jan 27:2021.01.20.21249279. doi: 
            10.1101/2021.01.20.21249279."
COMMENT     Sequence Label: pET30a-FluB-NP
FEATURES             Location/Qualifiers
     source          1..1677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1465..1476
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atgagcaaca tggacattga tggtatgaac accggcacca ttgacaagac cccggaggaa
       61 atcaccagcg gtaccagcgg taccacccgt ccgatcattc gtccggcgac cctggcgccg
      121 ccgagcaaca agcgtacccg taacccgagc ccggagcgtg cgaccaccag cagcgaagac
      181 gatgttggtc gtaaagcgca gaagaaacaa accccgaccg agatcaagaa aagcgtgtac
      241 aacatggtgg ttaagctggg tgaattctat aaccagatga tggttaaagc gggcctgaac
      301 gacgatatgg agcgtaacct gattcaaaac gcgcacgcgg tggaacgtat cctgctggcg
      361 gcgaccgacg ataagaaaac cgagtttcag aagaaaaaga acgcgcgtga cgtgaaagaa
      421 ggcaaggagg aaattgatca caacaagacc ggtggcacct tctacaagat ggttcgtgac
      481 gataaaacca tctattttag cccgatccgt attaccttcc tgaaagagga agtgaagacc
      541 atgtacaaaa ccaccatggg cagcgacggt tttagcggcc tgaaccacat catgattggc
      601 cacagccaga tgaacgacgt gtgcttccaa cgtagcaaag cgctgaagcg tgttggtctg
      661 gacccgagcc tgattagcac ctttgcgggc agcaccgttc cgcgtcgtag cggtgcgacc
      721 ggtgtggcga tcaaaggtgg cggtaccctg gttgcggaag cgattcgttt catcggtcgt
      781 gcgatggcgg accgtggcct gctgcgtgat attaaagcga agaccgcgta tgagaagatc
      841 ctgctgaacc tgaaaaacaa gtgcagcgcg ccgcagcaaa aagcgctggt tgatcaggtg
      901 atcggtagcc gtaacccggg cattgcggac atcgaagatc tgaccctgct ggcgcgtagc
      961 atggtggttg tgcgtccgag cgttgcgagc aaggttgtgc tgccgatcag catttacgcg
     1021 aaaattccgc agctgggttt taacgttgag gaatacagca tggtgggcta tgaggcgatg
     1081 gcgctgtata acatggcgac cccggttagc atcctgcgta tgggtgacga tgcgaaagac
     1141 aagagccaac tgttctttat gagctgcttc ggcgcggcgt acgaggatct gcgtgtgctg
     1201 agcgcgctga ccggtaccga atttaaaccg cgtagcgcgc tgaaatgcaa gggcttccac
     1261 gttccggcga aggagcaggt ggaaggcatg ggtgcggcgc tgatgagcat taaactgcag
     1321 ttttgggcgc cgatgacccg tagcggtggt aacgaggcgg gtggtgacgg tggtagcggt
     1381 cagattagct gcagcccggt tttcgcggtg gaacgtccga tcgcgctgag caagcaagcg
     1441 gtgcgtcgta tgctgagcat gaacatcgag ggtcgtgacg cggatgttaa gggcaacctg
     1501 ctgaaaatga tgaacgacag catggcgaaa aagaccagcg gtaacgcgtt tattggcaaa
     1561 aagatgttcc agatcagcga taaaaacaag accaacccga tcgagattcc gatcaaacaa
     1621 accatcccga acttcttttt cggtcgtgac accgcggaag attatgacga tctggat
//
