LOCUS       sequence-320732-        6474 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty vector control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6474)
  AUTHORS   Hatterschide J, Brantly AC, Grace M, Munger K, White EA
  TITLE     A Conserved Amino Acid in the C Terminus of Human Papillomavirus E7 
            Mediates Binding to PTPN14 and Repression of Epithelial 
            Differentiation.
  JOURNAL   J Virol. 2020 Aug 17;94(17). pii: JVI.01024-20. doi: 
            10.1128/JVI.01024-20. Print 2020 Aug 17.
  PUBMED    32581101
REFERENCE   2  (bases 1 to 6474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2020-08-17"; volume: "94(17). pii: JVI.01024-20. doi"; pages:
            " 10.1128/JVI.01024-20. Print 2020 Aug 17"
COMMENT     Sequence Label: p8208 MSCV-Neo C-HA EMPTY
FEATURES             Location/Qualifiers
     source          1..6474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             31..57
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             31..57
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        70..569
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(105..128)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     502..521
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             590..1384
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             590..1384
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(644..663)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1254..1273
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             1463..1977
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    2139..2155
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2163..2169,2170..2187,2188..2193))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2208..2229
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2346..2363)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2517..3105)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2597..2616)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3276..4067,4068..4136))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3899..3918
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4137..4241)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4309..4327
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4365..4387)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4487..4506
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4700..4722
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4850..4869
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             5069..5585
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    5649..5990
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             6057..6473
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     6403..6425
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     6441..6457
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 gaattagatc tcgagtcggc cgctggagga tacccctacg acgtgcccga ctacgcctag
       61 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
      121 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
      181 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
      241 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
      301 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
      361 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
      421 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
      481 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
      541 tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca tgattgaaca
      601 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
      661 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
      721 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
      781 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
      841 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
      901 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
      961 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     1021 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     1081 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct
     1141 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     1201 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     1261 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     1321 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     1381 ctgacgcccg accgaaagga gcgcacgacc ccatgcatcg ataaaataaa agattttatt
     1441 tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta
     1501 agtaacgcca ttttgcaagg catggaaaat acataactga gaatagagaa gttcagatca
     1561 aggttaggaa cagagagaca gcagaatatg ggccaaacag gatatctgtg gtaagcagtt
     1621 cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtcccgc cctcagcagt
     1681 ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct
     1741 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag
     1801 ctcaataaaa gagcccacaa cccctcactc ggcgcgccag tcctccgata gactgcgtcg
     1861 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct
     1921 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatggg
     1981 taacagtttc ttgaagttgg agaacaacat tctgagggta ggagtcgaat attaagtaat
     2041 cctgactcaa ttagccactg ttttgaatcc acatactcca atactcctga aatagttcat
     2101 tatggacagc gcagaaagag ctggggagaa ttgtgaaatt gttatccgct cacaattcca
     2161 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     2221 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     2281 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     2341 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     2401 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     2461 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     2521 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     2581 aacccgacag gactataaag ataccaggcg tttccccttg gaagctccct cgtgcgctct
     2641 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     2701 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     2761 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     2821 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     2881 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     2941 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     3001 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     3061 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     3121 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     3181 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     3241 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     3301 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     3361 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     3421 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     3481 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     3541 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     3601 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     3661 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     3721 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     3781 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     3841 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     3901 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     3961 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     4021 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     4081 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     4141 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     4201 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     4261 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     4321 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     4381 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     4441 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     4501 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     4561 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     4621 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     4681 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggcgca aggaatggtg
     4741 catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc
     4801 cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg
     4861 cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg
     4921 cgtagaggcg attagtccaa tttgttaaag acaggatatc agtggtccag gctctagttt
     4981 tgactcaaca atatcaccag ctgaagccta tagagtacga gccatagata aaataaaaga
     5041 ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc
     5101 tagcttaagt aacgccattt tgcaaggcat ggaaaataca taactgagaa tagagaagtt
     5161 cagatcaagg ttaggaacag agagacagca gaatatgggc caaacaggat atctgtggta
     5221 agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg gtcccgccct
     5281 cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc
     5341 tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct
     5401 ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc tccgatagac
     5461 tgcgtcgccc gggtacccgt attcccaata aagcctcttg ctgtttgcat ccgaatcgtg
     5521 gactcgctga tccttgggag ggtctcctca gattgattga ctgcccacct cgggggtctt
     5581 tcatttggag gttccaccga gatttggaga cccctgccca gggaccaccg acccccccgc
     5641 cgggaggtaa gctggccagc ggtcgtttcg tgtctgtctc tgtctttgtg cgtgtttgtg
     5701 ccggcatcta atgtttgcgc ctgcgtctgt actagttagc taactagctc tgtatctggc
     5761 ggacccgtgg tggaactgac gagttctgaa cacccggccg caaccctggg agacgtccca
     5821 gggactttgg gggccgtttt tgtggcccga cctgaggaag ggagtcgatg tggaatccga
     5881 ccccgtcagg atatgtggtt ctggtaggag acgagaacct aaaacagttc ccgcctccgt
     5941 ctgaattttt gctttcggtt tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca
     6001 gcatcgttct gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa aattagggcc
     6061 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
     6121 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
     6181 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     6241 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     6301 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     6361 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     6421 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gccg
//
