LOCUS       sequence-320753-        3195 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of a GST fusion of Human Uba1 with a 6alanine 
            linker between the GST encoded by the plasmid and the inserted Uba1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3195)
  TITLE     Haas Lab plasmids
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGEX4T-1-6AlanineHE1
FEATURES             Location/Qualifiers
     source          1..3195
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gctgcagctg cagcagctat gtccagctcg ccgctgtcca agaaacgtcg cgtgtccggg
       61 cctgatccaa agccgggttc taactgctcc cctgcccagt ccgtgttgtc cgaagtgccc
      121 tcggtgccaa ccaacggaat ggccaagaac ggcagtgaag cagacataga cgagggcctt
      181 tactcccggc agctgtatgt gttgggccat gaggcaatga agcggctcca gacatccagt
      241 gtcctggtat caggcctgcg gggcctgggc gtggagatcg ctaagaacat catccttggt
      301 ggggtcaagg ctgttaccct acatgaccag ggcactgccc agtgggctga tctttcctcc
      361 cagttctacc tgcgggagga ggacatcggt aaaaaccggg ccgaggtatc acagccccgc
      421 ctcgctgagc tcaacagcta tgtgcctgtc actgcctaca ctggacccct cgttgaggac
      481 ttccttagtg gtttccaggt ggtggtgctc accaacaccc ccctggagga ccagctgcga
      541 gtgggtgagt tctgtcacaa ccgtggcatc aagctggtgg tggcagacac gcggggcctg
      601 tttgggcagc tcttctgtga ctttggagag gaaatgatcc tcacagattc caatggggag
      661 cagccactca gtgctatggt ttctatggtt accaaggaca accccggtgt ggttacctgc
      721 ctggatgagg cccgacacgg gtttgagagc ggggactttg tctccttttc agaagtacag
      781 ggcatggttg aactcaacgg aaatcagccc atggagatca aagtcctggg tccttatacc
      841 tttagcatct gtgacacctc caacttctcc gactacatcc gtggaggcat cgtcagtcag
      901 gtcaaagtac ctaagaagat tagctttaaa tccttggtgg cctcactggc agaacctgac
      961 tttgtggtga cggacttcgc caagttttct cgccctgccc agctgcacat tggcttccag
     1021 gccctgcacc agttctgtgc tcagcatggc cggccacctc ggccccgcaa tgaggaggat
     1081 gcagcagaac tggtagcctt agcacaggct gtgaatgctc gagccctgcc agcagtgcag
     1141 caaaataacc tggacgagga cctcatccgg aagctggcat atgtggctgc tggggatctg
     1201 gcacccataa acgccttcat tgggggcctg gctgcccagg aagtcatgaa ggcctgctcc
     1261 gggaagttca tgcccatcat gcagtggcta tactttgatg cccttgagtg tctccctgag
     1321 gacaaagagg tcctcacaga ggacaagtgc ctccagcgcc agaaccgtta tgacgggcaa
     1381 gtggctgtgt ttggctcaga cctgcaagag aagctgggca agcagaagta tttcctggtg
     1441 ggtgcggggg ccattggctg tgagctgctc aagaactttg ccatgattgg gctgggctgc
     1501 ggggagggtg gagaaatcat cgttacagac atggacacca ttgagaagtc aaatctgaat
     1561 cgacagtttc ttttccggcc ctgggatgtc acgaagttaa agtctgacac ggctgctgca
     1621 gctgtgcgcc aaatgaatcc acatatccgg gtgacaagcc accagaaccg tgtgggtcct
     1681 gacacggagc gcatctatga tgacgatttt ttccaaaacc tagatggcgt ggccaatgcc
     1741 ctggacaacg tggatgcccg catgtacatg gaccgccgct gtgtctacta ccggaagcca
     1801 ctgctggagt caggcacact gggcaccaaa ggcaatgtgc aggtggtgat ccccttcctg
     1861 acagagtcgt acagttccag ccaggaccca cctgagaagt ccatccccat ctgtaccctg
     1921 aagaacttcc ctaatgccat cgagcacacc ctgcagtggg ctcgggatga gtttgaaggc
     1981 ctcttcaagc agccagcaga aaatgtcaac cagtacctca cagaccccaa gtttgtggag
     2041 cgaacactgc ggctggcagg cactcagccc ttggaggtgc tggaggctgt gcagcgcagc
     2101 ctggtgctgc agcgaccaca gacctgggct gactgcgtga cctgggcctg ccaccactgg
     2161 cacacccagt actcgaacaa catccggcag ctgctgcaca acttccctcc tgaccagctc
     2221 acaagctcag gagcgccgtt ctggtctggg cccaaacgct gtccacaccc gctcaccttt
     2281 gatgtcaaca atcccctgca tctggactat gtgatggctg ctgccaacct gtttgcccag
     2341 acctacgggc tgacaggctc tcaggaccga gctgctgtgg ccacattcct gcagtctgtg
     2401 caggtccccg aattcacccc caagtctggc gtcaagatcc atgtttctga ccaggagctg
     2461 cagagcgcca atgcctctgt tgatgacagt cgtctagagg agctcaaagc cactctgccc
     2521 agcccagaca agctccctgg attcaagatg taccccattg actttgagaa ggatgatgac
     2581 agcaactttc atatggattt catcgtggct gcatccaacc tccgggcaga aaactatgac
     2641 attccttctg cagaccggca caagagcaag ctgattgcag ggaagatcat cccagccatt
     2701 gccacgacca cagcagccgt ggttggcctt gtgtgtctgg agctgtacaa ggttgtgcag
     2761 gggcaccgac agcttgactc ctacaagaat ggtttcctca acttggccct gcctttcttt
     2821 ggtttctctg aaccccttgc cgcaccacgt caccagtact ataaccaaga gtggacattg
     2881 tgggatcgct ttgaggtaca agggctgcag cctaatggtg aggagatgac cctcaaacag
     2941 ttcctcgact attttaagac agagcacaaa ttagagatca ccatgctgtc ccagggcgtg
     3001 tccatgctct attccttctt catgccagct gccaagctca aggaacggtt ggatcagccg
     3061 atgacagaga ttgtgagccg tgtgtcgaag cgaaagctgg gccgccacgt gcgggcgctg
     3121 gtgcttgagc tgtgctgtaa cgacgagagc ggcgaggatg tcgaggttcc ctatgtccga
     3181 tacaccatcc gctga
//
