LOCUS       sequence-320796-        9137 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for stable expression of N-terminal 2xStrep-tagged
            UL52 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9137)
  AUTHORS   Didychuk AL, Gates SN, Gardner MR, Strong LM, Martin A, Glaunsinger 
            BA
  TITLE     A pentameric protein ring with novel architecture is required for 
            herpesviral packaging.
  JOURNAL   Elife. 2021 Feb 8;10. pii: 62261. doi: 10.7554/eLife.62261.
  PUBMED    33554858
REFERENCE   2  (bases 1 to 9137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-02-8"; volume: "10. pii: 62261. doi"; pages: " 
            10.7554/eLife.62261"
COMMENT     Sequence Label: pLJM1-zeo-2xStrep-UL52
FEATURES             Location/Qualifiers
     source          1..9137
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        131..334
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     285..305
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             374..397
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             434..457
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     misc_feature    2514..2631
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2680..3190
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3109..3127
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3199..3573
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     LTR             3645..3878
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3950..4071
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3987..4006)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4041..4060
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4111..4246
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4266..4285)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4267..4285)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4295..4312)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4295..4311)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4304..4326)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4453..4908
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4540..4559)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4750..4771
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4934..5038
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5039..5107,5108..5899)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5257..5276)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6070..6658
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6559..6578
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6812..6829
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6946..6967
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6982..6987,6988..7005,7006..7012)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7020..7036
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7025..7047
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7044..7060
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7044..7060
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7079..7099
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7081..7099
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7127..7353
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7354..7534
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7581..7706
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8199..8432
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8617..8661
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     8959..8979
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      361 gcgctaccgg atgtggagcc acccccagtt cgagaagggc ggcggcagcg gcggcggcag
      421 cggcggcggc agctggagcc acccccagtt cgagaagggg gcggccatga atccgagtac
      481 ccacgtgagc agtaacggcc caacgactcc ccctcacggg ccccacacca cgtttcttcc
      541 cccgaccagc ccggccccgt ccaccagctc cgtcgccgcc gctaccttgt gcagtccgca
      601 acgacaggcc gtttcacgtt acagcggctg gagcaccgag tacacccagt ggcactcgga
      661 cttgacaact gagctgctat ggcacgcgca cccacgtcaa gtacctatgg acgaagcgct
      721 ggccgccgcg gcggccgcct cataccaggt gaatcctcaa caccccgcca accgttaccg
      781 tcattacgaa ttccagacgc tcagtctcgg cacctcggag gtagacgaac tgctcaactg
      841 ctgtgcggaa gaaaccacgt gcggcggcac gcaatccacc gtactcacca atgcgaccaa
      901 caccactaac tgcggcggag ccgtcgccgg cagtagcaac gcaggacccg ccggcgcttc
      961 ggccgcctgc gacctagatg cagaactggc cggcctcgaa acctcggcgg ccgactttga
     1021 acaactgcgg cgactgtgcg cgccgctggc catcgacaca cgctgtaacc tatgcgccat
     1081 catcagcatc tgcctcaaac aagactgcga ccagagctgg ctcctcgagt acagcttgct
     1141 gtgcttcaaa tgcagttacg cgccccgtgc ggcgctcagc acgctcatca tcatgtccga
     1201 gtttacgcat ctgctgcagc agcacttttc cgatctgcgc atcgacgacc tgttccgaca
     1261 ccacgttctc acggtcttcg atttccacct gcacttcttc ataaatcgtt gctttgaaaa
     1321 acaagtgggc gacgcggttg ataacgagaa tgtcaccctg aaccatctgg ccgtggtgcg
     1381 ggccatggtc atgggcgaag acacggtgcc ttacaacaag cctcggcgcc acccgcaaca
     1441 gaagcaaaaa accaaccctt atcacgtcga agtgccgcaa gaactgatcg acaactttct
     1501 agaacacagc tcacctagcc gcgaccgctt cgtgcagctg cttttctata tgtgggccgg
     1561 caccggcgtc atgagcacca cgccactcac ggaactcacg cacactaagt tcgcgcgact
     1621 agacgcgtta tccacgacct cggaaagaga agacgcaagg atgatgatgg aagaagagga
     1681 agatgaagaa ggaggagaaa aaggaggaga cgatccgggc cgtcacaacg gcggtggcac
     1741 cagcgggggg ttcagcgaga gcacgctaaa aaagaacgtg ggtcccattt acctatgtcc
     1801 cgtacccgcc ttttttacca agaaccaaac cagtaccgtg tgtctgctgt gcgaactcat
     1861 ggcctgctcc tattacgata acgtcgtcct gcgcgaactg taccgtcgcg tcgtctcgta
     1921 ctgtcagaac aatgtgaaga tggtggaccg cattcagctg gtattggccg acctgttgcg
     1981 cgaatgcacg tcgccgctcg gcgcggcgca cgaggacgtg gcgcgctgtg gactcgaagc
     2041 gcccacctcg cccggaggcg actcggacta tcacggcctg agcggcgtcg acggcgcact
     2101 ggcgcgaccc gacccggtat tttgccacgt cctgcgtcaa gcgggcgtca cgggcatcta
     2161 caagcacttt ttctgtgacc cgcagtgcgc cggcaacatc cgcgtcacca acgaggccgt
     2221 gctcttcgga cgcctgcacc cccaccacgt ccaggaggtg aaactggcca tctgtcacga
     2281 caattactat ataagtcgac ttccgcgacg tgtgtggctc tgcatcacac tcttcaaggc
     2341 ctttcagatt acaaaacgca cctacaaagg caaagtgcac ctggcggact ttatgcgcga
     2401 tttcacgcag ctgttggaga gttgcgacat caagctggtg gaccccacgt acgtgataga
     2461 caagtatgtc taaaattctc gacctcgaga caaatggcag tattcatcca caattttaaa
     2521 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2581 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     2641 tacagggaca gcagagatcc actttggccg cggctcgagg gggttggggt tgcgcctttt
     2701 ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg
     2761 cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat
     2821 cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga
     2881 aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag
     2941 taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt
     3001 ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg
     3061 ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt
     3121 ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct
     3181 ctctccccag ggggatccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
     3241 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
     3301 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
     3361 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
     3421 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
     3481 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
     3541 tgcgtgcact tcgtggccga ggagcaggac tgaggtacct ttaagaccaa tgacttacaa
     3601 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     3661 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     3721 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     3781 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     3841 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     3901 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     3961 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     4021 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     4081 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     4141 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     4201 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     4261 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     4321 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     4381 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     4441 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4501 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     4561 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     4621 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     4681 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     4741 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     4801 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     4861 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     4921 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     4981 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     5041 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5101 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5161 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     5221 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     5281 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     5341 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     5401 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     5461 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     5521 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     5581 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     5641 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     5701 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     5761 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     5821 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     5881 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     5941 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6001 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6061 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6121 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6181 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     6241 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6301 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6361 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6421 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6481 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6541 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6601 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6661 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     6721 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     6781 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     6841 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     6901 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     6961 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     7021 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     7081 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
     7141 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
     7201 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
     7261 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
     7321 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
     7381 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     7441 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7501 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     7561 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     7621 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     7681 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     7741 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     7801 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     7861 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     7921 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     7981 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     8041 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     8101 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     8161 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     8221 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     8281 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     8341 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     8401 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     8461 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     8521 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     8581 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     8641 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     8701 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     8761 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     8821 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     8881 gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagcctcga
     8941 gcggccgccc ccttcaccga gggcctattt cccatgattc cttcatattt gcatatacga
     9001 tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta
     9061 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
     9121 tttaaaatgg actatca
//
