LOCUS       sequence-32081-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Villefranc JA, Nicoli S, Bentley K, Jeltsch M, Zarkada G, Moore JC, 
            Gerhardt H, Alitalo K, Lawson ND
  TITLE     A truncation allele in vascular endothelial growth factor c reveals 
            distinct modes of signaling during lymphatic and vascular 
            development.
  JOURNAL   Development. 2013 Apr;140(7):1497-506. doi: 10.1242/dev.084152. Epub
            2013 Mar 5.
  PUBMED    23462469
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.084152"; journalName: "Development"; date: "2013-04"; 
            volume: "140"; issue: "7"; pages: "1497-506"
COMMENT     Sequence Label: 475 p5EfliEBSbasP
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    21..116
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    335..458
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(530..549)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(531..549)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(554..570)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(554..570)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(672..691)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(750..769)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ttacgctacc atggagctcc aaataatgat tttattttga ctgatagtga cctgttcgtt
       61 gcaacaaatt gataagcaat gcttttttat aatgccaact ttgtatagaa aagttgtaca
      121 cactttagtc gacaatgaga agggtgtctc aatctatggg tatttcctgt tccacacaat
      181 ctggtttcct gtcaggtttc cttttcctcc tgaaacaaaa gacaggaatg ccctctgtat
      241 aaaatagcac agcgctttta atgggtctct gtgtactaga gggtatataa tggatcccat
      301 cgcgtctcag cctcactttg agctcctcca cacgcaagtt tgtacaaaaa agttgaacga
      361 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact
      421 acataatact gtaaaacaca acatatgcag tcactatgaa ccaactactt agatggtatt
      481 agtgacctgt agaattcgag ctctagagct gcagggcggc cgcgatatcc cctatagtga
      541 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg
      601 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
      661 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt
      721 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
      781 atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa
      841 acatggcaaa ggtagcgttg c
//
