LOCUS       sequence-32087-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Nicoli S, Standley C, Walker P, Hurlstone A, Fogarty KE, Lawson ND
  TITLE     MicroRNA-mediated integration of haemodynamics and Vegf signalling 
            during angiogenesis.
  JOURNAL   Nature. 2010 Apr 22. 464(7292):1196-200.
  PUBMED    20364122
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Apr 22. 464(7292):1196-200."
COMMENT     Sequence Label: 808 p3EmirCherry
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(59..182)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             192..899
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(337..355)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     605..624
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gggcctgcgc tctgagctcg aattctacag gtcactaata ccatctaagt agttggttca
       61 tagtgactgc atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta
      121 atttaatata ttgatattta tatcatttta cgtttctcgt tcaactttct tgtacaaagt
      181 ggggatccac catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
      241 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
      301 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      361 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
      421 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
      481 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
      541 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
      601 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
      661 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
      721 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
      781 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
      841 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
//
