LOCUS       sequence-32088-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Sato T, Nakashima A, Guo L, Coffman K, Tamanoi F
  TITLE     Single amino-acid changes that confer constitutive activation of 
            mTOR are discovered in human cancer.
  JOURNAL   Oncogene. 2010 May 6. 29(18):2746-52.
  PUBMED    20190810
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene. 
            2010 May 6. 29(18):2746-52."
COMMENT     Sequence Label: pcDNA3-Au1-mTOR-Wild type
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      93..102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cattagcgag ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata
       61 cgactcacta tagggagacc caagcttctc gagccaccat ggacacgtac aggtacatcc
      121 ttgggacagg ccctgccacg gccaccgccg gtgccgccac atccagcaac gtgagcgttc
      181 tgcagcagtt cgccagtggg ctgaagagcc ggaatgagga gaccagggcc aaagcagcca
      241 aggagctcca gcactatgtc accatggaac ttcgagagat gagtcaggag gagtctactc
      301 gcttctatga ccagctgaac catcacattt ttgaactggt ttccagctca gacgccaatg
      361 agaggaaggg tggcatcttg gccattgcca gcctcattgg agtggaaggt gggaattcca
      421 ccaggattgg cagatttgcc aactaccttc ggaacctcct cccctcaagt gatccagttg
      481 tcatggagat ggcatccaag gccattggcc gccttgcaat ggcaggggac actttcactg
      541 ctgagtatgt ggagtttgaa gtgaagcgag ccttggagtg gctgggtgct gaccgaaatg
      601 agggccggag acatgcagct gtcctcgttc tccgtgagct ggccatcagc gtccccacct
      661 tcttcttcca gcaagttcag cccttctttg acaacatttt tgtggctgtg tgggacccca
      721 agcaggccat ccgtgaagga gctgtggctg cccttcgtgc ctgtctgatt cttaccacgc
      781 agcgggagcc gaaggagatg cagaagcctc agtggtacag gcacacattt gaagaagcag
      841 agaaaggttt tgatgagacc ctggccaaag agaagggtat gaaccgagat gaca
//
