LOCUS       sequence-32139-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SGC Empty backbone for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  TITLE     Cloning Vectors
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCW-LIC
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(159..164,165..182,183..189)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    197..213
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(268..273,274..289,290..296)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    304..320
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(363..368,369..384,385..391)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    399..415
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    complement(488..521)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcaccat
       61 tcgatggtgt cctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      121 attaatgtaa gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      181 cgtataatgt gtggaattgt gagcggataa caatttcaca caggaaacag gatcgatcca
      241 tcgatgagct tactccccat ccccctgttg acaattaatc atcggctcgt ataatgtgtg
      301 gaattgtgag cggataacaa tttcacacag gaaacaggat cagcttactc cccatccccc
      361 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      421 cacaggaaac aggatccatc gatgcttagg aggtcatatg attatgagtt ctcctcctga
      481 aagatccata acttcgtata gcatacatta tacgaagtta tgcggccgcg acgtccacat
      541 atacctgccg ttcactatta tttagtgaaa tgagatatta tgatattttc tgaattgtga
      601 ttaaaaaggc aactttatgc ccatgcaaca gaaactataa aaaatacaga gaatgaaaag
      661 aaacagatag attttttagt tctttaggcc cgtagtctgc aaatcctttt atgattttct
      721 atcaaacaaa agaggaaaat agaccagttg caatccaaac gagagtctaa tagaatgagg
      781 tcgaaaagta aatcgcgcgg gtttgttact gataaagcag gcaagaccta aaatgtgtaa
      841 agggcaaagt gtatactttg gcgtca
//
