LOCUS       sequence-321569-       12331 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to construct lentivirus to express nucleus-targeted mCherry in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12331)
  TITLE     Expression plasmid for production of murine IFN gamma
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: nuc-mCherry-pTRIP
FEATURES             Location/Qualifiers
     source          1..12331
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2054..2171
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2230..2533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2534..2737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2688..2708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2734..2758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2835..2855
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2859..2879
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2883..2903
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2937..3644
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3082..3100)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3350..3369
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3651..3677
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     promoter        3744..4243
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3779..3802)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4176..4195
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4264..4863
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4264..4283)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4760..4780
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4877..5465
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4930..4950)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5348..5359)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5751..5931
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7459..7478)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7692..7711)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7811..7833
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7871..7889)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7957..8061
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8062..8130,8131..8922)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8280..8299)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9093..9681
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9582..9601
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9835..9852
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9922..9942)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9927..10256
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10107..10242
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11390..11455
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11585..11605
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12030..12164
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12067..12086)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12121..12140
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gccgaattca caaatggcag
     2041 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2101 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     2161 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggggc gataagcttg
     2221 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2281 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2341 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2401 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2461 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2521 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2581 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2641 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     2701 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     2761 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactct agaggccggc
     2821 caatcgatat ggatccaaaa aagaagagaa aggtagatcc aaaaaagaag agaaaggtag
     2881 atccaaaaaa gaagagaaag gtaggtcgac atcgcgagct agcgctaccg gtcaccatgg
     2941 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
     3001 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     3061 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
     3121 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
     3181 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     3241 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
     3301 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
     3361 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
     3421 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
     3481 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
     3541 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
     3601 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagacgcgt cctgctgcta
     3661 agagagtgaa actggattaa acgcgttaac tcgagaaccc gggaaggatc caccggatct
     3721 agataactga tcataattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
     3781 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
     3841 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
     3901 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
     3961 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
     4021 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
     4081 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
     4141 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
     4201 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt
     4261 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     4321 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     4381 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     4441 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     4501 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     4561 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     4621 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     4681 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     4741 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     4801 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     4861 tgaccgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     4921 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     4981 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     5041 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     5101 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     5161 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     5221 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     5281 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     5341 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     5401 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     5461 cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat cacaagtagc
     5521 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     5581 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     5641 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     5701 caaaatcgtc gagagatgct gcatataagc agctgctttt tgcttgtact gggtctctct
     5761 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     5821 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     5881 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     5941 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     6001 ggccttgaca ttataataga tttagcagga attgaactag gagtggagca cacaggcaaa
     6061 gctgcagaag tacttggaag aagccaccag agatactcac gattctgcac atacctggct
     6121 aatcccagat cctaaggatt acattaagtt tactaacatt tatataatga tttatagttt
     6181 aaagtataaa cttatctaat ttactattct gacagatatt aattaatcct caaatatcat
     6241 aagagatgat tactattatc cccatttaac acaagaggaa actgagaggg aaagatgttg
     6301 aagtaatttt cccacaatta cagcatccgt tagttacgac tctatgatct tctgacacaa
     6361 attccattta ctcctcaccc tatgactcag tcgaatatat caaagttatg gacattatgc
     6421 taagtaacaa attacccttt tatatagtaa atactgagta gattgagaga agaaattgtt
     6481 tgcaaacctg aatagcttca agaagaagag aagtgaggat aagaataaca gttgtcattt
     6541 aacaagtttt aacaagtaac ttggttagaa agggattcaa atgcataaag caagggataa
     6601 atttttctgg caacaagact atacaatata accttaaata tgacttcaaa taattgttgg
     6661 aacttgataa aactaattaa atattattga agattatcaa tattataaat gtaatttact
     6721 tttaaaaagg gaacatagaa atgtgtatca ttagagtaga aaacaatcct tattatcaca
     6781 atttgtcaaa acaagtttgt tattaacaca agtagaatac tgcattcaat taagttgact
     6841 gcagattttg tgttttgtta aaattagaaa gagataacaa caatttgaat tattgaaagt
     6901 aacatgtaaa tagttctaca tacgttcttt tgacatcttg ttcaatcatt gatcgaagtt
     6961 ctttatcttg gaagaatttg ttccaaagac tctgaaataa ggaaaacaat ctattatata
     7021 gtctcacacc tttgttttac ttttagtgat ttcaatttaa taatgtaaat ggttaaaatt
     7081 tattcttctc tgagatcatt tcacattgca gatagaaaac ctgagactgg ggtaattttt
     7141 attaaaatct aatttaatct cagaaacaca tctttattct aacatcaatt tttccagttt
     7201 gatattatca tataaagtca gccttcctca tctgcaggtt ccacaacaaa aatccaacca
     7261 actgtggatc aaaaatattg ggaaaaaatt aaaaatagca atacaacaat aaaaaaatac
     7321 aaatcagaaa aacagcacag tataacaact ttatttagca tttacaatct attaggtatt
     7381 ataagtaatc tagccagatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca
     7441 caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc
     7501 acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg
     7561 gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc
     7621 tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaatgg
     7681 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     7741 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     7801 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     7861 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     7921 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     7981 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     8041 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     8101 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     8161 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     8221 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     8281 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     8341 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     8401 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     8461 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     8521 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     8581 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     8641 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     8701 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     8761 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     8821 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     8881 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     8941 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     9001 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     9061 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     9121 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     9181 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     9241 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     9301 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     9361 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     9421 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     9481 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     9541 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     9601 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     9661 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     9721 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     9781 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     9841 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     9901 ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     9961 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
    10021 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
    10081 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
    10141 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
    10201 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
    10261 ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg
    10321 agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat
    10381 ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag
    10441 gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat
    10501 gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta
    10561 agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg
    10621 ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac
    10681 gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta
    10741 ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac
    10801 cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg
    10861 aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc
    10921 aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca
    10981 gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa
    11041 ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa
    11101 ccgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac
    11161 cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt
    11221 attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac
    11281 cgtggcggca actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg
    11341 tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg taaatataaa
    11401 atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc
    11461 aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt
    11521 ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac
    11581 tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag
    11641 ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac
    11701 aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat
    11761 aagtaggcat aacagttata atcataacat actgtttttt cttactccac acaggcatag
    11821 agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa
    11881 aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata
    11941 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
    12001 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
    12061 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
    12121 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
    12181 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt
    12241 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
    12301 tttgttaaat atgtactaca aacttagtag t
//
