LOCUS       sequence-321670-       13785 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for the expression of the human codon optimized Cas9 and 
            kanamycin resistance (NptII)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13785)
  TITLE     Orzaez lab plasmids
REFERENCE   2  (bases 1 to 13785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDGB3 omega1 Tnos:nptII:Pnos - SF - P35S:hCas9...
FEATURES             Location/Qualifiers
     source          1..13785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(28..280)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(320..1114)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(431..450)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1041..1060
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1148..1327)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2029..2373
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2317..2337
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             2447..6550
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2447..6550
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2447..6550
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6563..6583
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6610..6862
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6897..6915)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    7104..7128
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8429..9058
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9487..10560
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10626..10820
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11056..11078)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11164..11304
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11219..11238
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11319..11336)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11490..12078)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11570..11589)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12166..12957)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    13743..13767
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagagcgat cagcttgcat gccggtcgat ctagtaacat agatgacacc gcgcgcgata
       61 atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg tataattgcg
      121 ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg ttaattatta
      181 catgcttaac gtaattcaac agaaattata tgataatcat ggcaagaccg gcaacaggat
      241 tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc tgcttgactc tagctagagt
      301 ccgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag gctatgcgct
      361 gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa
      421 gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca
      481 gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc
      541 aggcgtcgcc gtgggtcacg acgagatcct cgccgtcggg catccgcgcc ttgagcctgg
      601 cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa
      661 gaccggcttc catccgagta cgtgctcgct cgatgcggtg tttcgcttgg tggtcgaatg
      721 ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt
      781 tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca
      841 gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg
      901 tggccagcca cgatagccgc gctgcctcgt cttggagttc attcagggca ccggacaggt
      961 cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag
     1021 agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg
     1081 gagaacctgc gtgcaatcca tcttgttcaa tcatgcctcg atcgagttga gagtgaatat
     1141 gagactctaa ttggataccg aggggaattt atggaacgtc agtggagcat ttttgacaag
     1201 aaatatttgc tagctgatag tgaccttagg cgacttttga acgcgcaata atggtttctg
     1261 acgtatgtgc ttagctcatt aaactccaga aacccgcggc tcagtggctc cttcaacgtt
     1321 gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg ggggtcatac
     1381 cgtgactccc ttaattctca tgtatctccg tcaggagact agagccaagc tgatctcctt
     1441 tgccccggag atcaccatgg acgactttct ctatctctac gatctaggaa gaaagttcga
     1501 cggagaaggt gacgatacca tgttcaccac cgataatgag aagattagcc tcttcaattt
     1561 cagaaagaat gctgacccac agatggttag agaggcctac gcggcaggtc tgatcaagac
     1621 gatctacccg agtaataatc tccaggagat caaatacctt cccaagaagg ttaaagatgc
     1681 agtcaaaaga ttcaggacta actgcatcaa gaacacagag aaagatatat ttctcaagat
     1741 cagaagtact attccagtat ggacgattca aggcttgctt cataaaccaa ggcaagtaat
     1801 agagattgga gtctctaaga aagtagttcc tactgaatca aaggccatgg agtcaaaaat
     1861 tcagatcgag gatctaacag aactcgccgt gaagactggc gaacagttca tacagagtct
     1921 tttacgactc aatgacaaga agaaaatctt cgtcaacatg gtggagcacg acactctcgt
     1981 ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg agacttttca
     2041 acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
     2101 caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg ataaaggaaa
     2161 ggctatcgtt caagatgccc ctgccgacag tggtcccaaa gatggacccc cacccacgag
     2221 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
     2281 tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
     2341 tatataagga agttcatttc atttggagag gactccggta tttttacaac aattaccaca
     2401 acaaaacaaa caacaaacaa cattacaatt tactattcta gtcgaaatgg acaagaagta
     2461 ctccattggg ctcgatatcg gcacaaacag cgtcggctgg gccgtcatta cggacgagta
     2521 caaggtgccg agcaaaaaat tcaaagttct gggcaatacc gatcgccaca gcataaagaa
     2581 gaacctcatt ggcgccctcc tgttcgactc cggggaaacg gccgaagcca cgcggctcaa
     2641 aagaacagca cggcgcagat atacccgcag aaagaatcgg atctgctacc tgcaggagat
     2701 ctttagtaat gagatggcta aggtggatga ctctttcttc cataggctgg aggagtcctt
     2761 tttggtggag gaggataaaa agcacgagcg ccacccaatc tttggcaata tcgtggacga
     2821 ggtggcgtac catgaaaagt acccaaccat atatcatctg aggaagaagc ttgtagacag
     2881 tactgataag gctgacttgc ggttgatcta tctcgcgctg gcgcatatga tcaaatttcg
     2941 gggacacttc ctcatcgagg gggacctgaa cccagacaac agcgatgtcg acaaactctt
     3001 tatccaactg gttcagactt acaatcagct tttcgaagag aacccgatca acgcatccgg
     3061 agttgacgcc aaagcaatcc tgagcgctag gctgtccaaa tcccggcggc tcgaaaacct
     3121 catcgcacag ctccctgggg agaagaagaa cggcctgttt ggtaatctta tcgccctgtc
     3181 actcgggctg acccccaact ttaaatctaa cttcgacctg gccgaagatg ccaagcttca
     3241 actgagcaaa gacacctacg atgatgatct cgacaatctg ctggcccaga tcggcgacca
     3301 gtacgcagac ctttttttgg cggcaaagaa cctgtcagac gccattctgc tgagtgatat
     3361 tctgcgagtg aacacggaga tcaccaaagc tccgctgagc gctagtatga tcaagcgcta
     3421 tgatgagcac caccaagact tgactttgct gaaggccctt gtcagacagc aactgcctga
     3481 gaagtacaag gaaattttct tcgatcagtc taaaaatggc tacgccggat acattgacgg
     3541 cggagcaagc caggaggaat tttacaaatt tattaagccc atcttggaaa aaatggacgg
     3601 caccgaggag ctgctggtaa agcttaacag agaagatctg ttgcgcaaac agcgcacttt
     3661 cgacaatgga agcatccccc accagattca cctgggcgaa ctgcacgcta tcctcaggcg
     3721 gcaagaggat ttctacccct ttttgaaaga taacagggaa aagattgaga aaatcctcac
     3781 atttcggata ccctactatg taggccccct cgcccgggga aattccagat tcgcgtggat
     3841 gactcgcaaa tcagaagaga ctatcactcc ctggaacttc gaggaagtcg tggataaggg
     3901 ggcctctgcc cagtccttca tcgaaaggat gactaacttt gataaaaatc tgcctaacga
     3961 aaaggtgctt cctaaacact ctctgctgta cgagtacttc acagtttata acgagctcac
     4021 caaggtcaaa tacgtcacag aagggatgag aaagccagca ttcctgtctg gagagcagaa
     4081 gaaagctatc gtggacctcc tcttcaagac gaaccggaaa gttaccgtga aacagctcaa
     4141 agaagattat ttcaaaaaga ttgaatgttt cgactctgtt gaaatcagcg gagtggagga
     4201 tcgcttcaac gcatccctgg gaacgtatca cgatctcctg aaaatcatta aagacaagga
     4261 cttcctggac aatgaggaga acgaggacat tcttgaggac attgtcctca cccttacgtt
     4321 gtttgaagat agggagatga ttgaagaacg cttgaaaact tacgctcatc tcttcgacga
     4381 caaagtcatg aaacagctca agaggcgccg atatacagga tgggggcggc tgtcaagaaa
     4441 actgatcaat gggatccgag acaagcagag tggaaagaca atcctggatt ttcttaagtc
     4501 cgatggattt gccaaccgga acttcatgca gttgatccat gatgactctc tcacctttaa
     4561 ggaggacatc cagaaagcac aagtttctgg ccagggggac agtctccacg agcacatcgc
     4621 taatcttgca ggtagcccag ctatcaaaaa gggaatactg cagaccgtta aggtcgtgga
     4681 tgaactcgtc aaagtaatgg gaaggcataa gcccgagaat atcgttatcg agatggcccg
     4741 agagaaccaa actacccaga agggacagaa gaacagtagg gaaaggatga agaggattga
     4801 agagggtata aaagaactgg ggtcccaaat ccttaaggaa cacccagttg aaaacaccca
     4861 gcttcagaat gagaagctct acctgtacta cctgcagaac ggcagggaca tgtacgtgga
     4921 tcaggaactg gacatcaatc ggctctccga ctacgacgtg gatcatatcg tgccccagtc
     4981 ttttctcaaa gatgattcta ttgataataa agtgttgaca agatccgata aaaatagagg
     5041 gaagagtgat aacgtcccct cagaagaagt tgtcaagaaa atgaaaaatt attggcggca
     5101 gctgctgaac gccaaactga tcacacaacg gaagttcgat aatctgacta aggctgaacg
     5161 aggtggcctg tctgagttgg ataaagccgg cttcatcaaa aggcagcttg ttgagacacg
     5221 ccagatcacc aagcacgtgg cccaaattct cgattcacgc atgaacacca agtacgatga
     5281 aaatgacaaa ctgattcgag aggtgaaagt tattactctg aagtctaagc tggtttcaga
     5341 tttcagaaag gactttcagt tttataaggt gagagagatc aacaattacc accatgcgca
     5401 tgatgcctac ctgaatgcag tggtaggcac tgcacttatc aaaaaatatc ccaagcttga
     5461 atctgaattt gtttacggag actataaagt gtacgatgtt aggaaaatga tcgcaaagtc
     5521 tgagcaggaa ataggcaagg ccaccgctaa gtacttcttt tacagcaata ttatgaattt
     5581 tttcaagacc gagattacac tggccaatgg agagattcgg aagcgaccac ttatcgaaac
     5641 aaacggagaa acaggagaaa tcgtgtggga caagggtagg gatttcgcga cagtccggaa
     5701 ggtcctgtcc atgccgcagg tgaacatcgt taaaaagacc gaagtacaga ccggaggctt
     5761 ctccaaggaa agtatcctcc cgaaaaggaa cagcgacaag ctgatcgcac gcaaaaaaga
     5821 ttgggacccc aagaaatacg gcggattcga ttctcctaca gtcgcttaca gtgtactggt
     5881 tgtggccaaa gtggagaaag ggaagtctaa aaaactcaaa agcgtcaagg aactgctggg
     5941 catcacaatc atggagcgat caagcttcga aaaaaacccc atcgactttc tcgaggcgaa
     6001 aggatataaa gaggtcaaaa aagacctcat cattaagctt cccaagtact ctctctttga
     6061 gcttgaaaac ggccggaaac gaatgctcgc tagtgcgggc gagctgcaga aaggtaacga
     6121 gctggcactg ccctctaaat acgttaattt cttgtatctg gccagccact atgaaaagct
     6181 caaaggatct cccgaagata atgagcagaa gcagctgttc gtggaacaac acaaacacta
     6241 ccttgatgag atcatcgagc aaataagcga attctccaaa agagtgatcc tcgccgacgc
     6301 taacctcgat aaggtgcttt ctgcttacaa taagcacagg gataagccca tcagggagca
     6361 ggcagaaaac attatccact tgtttactct gaccaacttg ggcgcgcctg cagccttcaa
     6421 gtacttcgac accaccatag acagaaagcg gtacacctct acaaaggagg tcctggacgc
     6481 cacactgatt catcagtcaa ttacggggct ctatgaaaca agaatcgacc tctctcagct
     6541 cggtggagac agcagggctg accccaagaa gaagaggaag gtgtgagctt ggaatggatc
     6601 ttcgatcccg atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
     6661 ggtcttgcga cgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
     6721 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     6781 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
     6841 gtgtcatcta tgttactaga tcgggaattg ccaagctaat tcttgaagac gaaagggcct
     6901 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     6961 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     7021 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat gggaccgact
     7081 cgcgctgtca tgagaccgga tcctgacagg atatattggc gggtaaacct aagagaaaag
     7141 agcgtttatt agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca
     7201 tttgtatgtg catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc
     7261 ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtcatc tgaaaacgac
     7321 atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt
     7381 cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac
     7441 gccatgaaca agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac
     7501 caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc
     7561 gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta
     7621 cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac
     7681 ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag
     7741 ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt
     7801 gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag
     7861 gcccgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc
     7921 cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg
     7981 catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc
     8041 aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc
     8101 gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt
     8161 ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc
     8221 ccgcgcacct ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc
     8281 tggcggcctg gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt
     8341 gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag
     8401 taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg
     8461 cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc
     8521 cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg
     8581 ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa
     8641 ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc
     8701 tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga
     8761 catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg
     8821 aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga
     8881 ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg
     8941 cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg
     9001 cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt
     9061 taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc
     9121 gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg
     9181 gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa
     9241 ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc
     9301 aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga
     9361 acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg
     9421 cgtaagcggc tgggttgcct gccggccctg caatggcact ggaaccccca agcccgagga
     9481 atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat
     9541 gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa
     9601 gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa
     9661 ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat
     9721 tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg
     9781 gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt
     9841 ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac
     9901 gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg
     9961 aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc
    10021 cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac
    10081 accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta
    10141 tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg
    10201 gagtacatcg agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac
    10261 ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt
    10321 ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg
    10381 atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag
    10441 ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc
    10501 ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa
    10561 tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggactc
    10621 tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg
    10681 tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata
    10741 aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa
    10801 acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg
    10861 cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc
    10921 gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc
    10981 gcgccgtcgc cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg
    11041 tgatgacggt gaaaacctct gacacatgca gctcccggtg acggtcacag cttgtctgta
    11101 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
    11161 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg
    11221 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
    11281 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc
    11341 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
    11401 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
    11461 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
    11521 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
    11581 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
    11641 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
    11701 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    11761 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    11821 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    11881 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
    11941 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    12001 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    12061 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    12121 aacgaaaact cacgttaagg gattttggtc atgcattcta ggtgattatt tgccgactac
    12181 cttggtgatc tcgcctttca cgtagtggac aaattcttcc aactgatctg cgcgcgaggc
    12241 caagcgatct tcttcttgtc caagataagc ctgtctagct tcaagtatga cgggctgata
    12301 ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc
    12361 ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcaccagc
    12421 ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg
    12481 ttcaggaacc ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc
    12541 tcttgctttt gtcagcaaga tagccagatc aatgtcgatc gtggctggct cgaagatacc
    12601 tgcaagaatg tcattgcgct gccattctcc aaattgcagt tcgcgcttag ctggataacg
    12661 ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga gaatctcgct
    12721 ctctccaggg gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc
    12781 atcaagcctt acggtcaccg taaccagcaa atcaatatca ctgtgtggct tcaggccgcc
    12841 atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat
    12901 gacgccaact acctctgata gttgagtcga tacttcggcg atcaccgctt ccctcataat
    12961 gtttaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tccataacat
    13021 caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac
    13081 cccaaaaaaa cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg
    13141 ttcggtcaag gttctggacc agttgcgtga gcgcatacgc tacttgcatt acagcttacg
    13201 aaccgaacag gcttatgtcc actgggttcg tgccttcatc cgtttccacg gtgtgcgtca
    13261 cccggcaacc ttgggtagca gcgaagtcga ggcatttctg tcctggctgg aacagaactt
    13321 attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa
    13381 gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt
    13441 ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc
    13501 gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga
    13561 gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac
    13621 atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg
    13681 ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct
    13741 ggtggcagga tatattgtgg tgtaaacata acggatccgg tctca
//
