LOCUS       sequence-321698-        7979 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein interaction screening tool with Gateway-compatible BiFC 
            vectors comprising of a set of 4 vectors permiting N or C fusions of
            candidate genes to the N or C fragment of Split-Venus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7979)
  AUTHORS   Lepur A, Kovacevic L, Beluzic R, Vugrek O
  TITLE     Combining Unique Multiplex Gateway Cloning and Bimolecular 
            Fluorescence Complementation (BiFC) for High-Throughput Screening of
            Protein-Protein Interactions.
  JOURNAL   J Biomol Screen. 2016 Dec;21(10):1100-1111. doi: 
            10.1177/1087057116659438. Epub 2016 Jul 28.
  PUBMED    27455993
REFERENCE   2  (bases 1 to 7979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1177/1087057116659438"; journalName: "J Biomol Screen"; date: 
            "2016-12"; volume: "21"; issue: "10"; pages: "1100-1111"
COMMENT     Sequence Label: pVenusNfN
FEATURES             Location/Qualifiers
     source          1..7979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        complement(834..938)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        1048..1351
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1352..1555
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1506..1526
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1552..1576
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(1674..1676,1677..1679,1680..2138)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
     primer_bind     complement(1719..1740)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1980..1999)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     protein_bind    2205..2329
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2354..2359,2360..2377,2378..2384)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2438..3097
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2504..2523)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3439..3744
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRVSRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3623..3642
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3785..3909)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3941..3960)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3942..3960)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3971..3988)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3971..3987)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3980..4002)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     polyA_signal    4392..4526
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4429..4448)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4483..4502
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4652..5107
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4739..4758)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4949..4970
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5224..5581
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5224..5415
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5247..5267)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5432..5567
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5632..6426
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAAELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5686..5705)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6296..6315
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6490..6538
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(6579..6598)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6698..6720
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        6844..6948
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6949..7017,7018..7809)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7167..7186)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttgccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtgtc cggtaagcgg cagggtcgga acaggagagc gcatgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cccctccccg cgcgttggcc gattcattaa tgcagagctt gcaattcgcg
      841 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
      901 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
      961 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtattacg
     1021 aggccctttc acgcattaga tgcatgtcgt tacataactt acggtaaatg gcccgcctgg
     1081 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     1141 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     1201 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     1261 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     1321 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     1381 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     1441 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     1501 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt
     1561 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
     1621 ccgggaccga tccagcctcc ggaccctagc ctaggccgcg gatgagtacc ggtatggtga
     1681 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     1741 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     1801 tgaccctgaa gctgatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     1861 ccaccctggg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
     1921 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     1981 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     2041 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     2101 agtacaacta caacagccac aacgtctata tcaccgccga caagcaaatc gatggtggcg
     2161 gtggctctgg aggtggtggg tcccttaaga cgcgtctcga gtcaacaagt ttgtacaaaa
     2221 aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata
     2281 aaaaacagac tacataatac tgtaaaacac aacatatcca gtcattatgg cggccgcatt
     2341 aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta
     2401 ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
     2461 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
     2521 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
     2581 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
     2641 tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
     2701 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
     2761 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
     2821 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa
     2881 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
     2941 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
     3001 gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
     3061 acaacagtac tgcgatgagt ggcagggcgg ggcgtaaaga tctgggtccg gcttactaaa
     3121 agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga
     3181 tatgtatacc cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca
     3241 gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg
     3301 taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa
     3361 atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg
     3421 agaacagggg ctggtgaaat gcagtttaag gtttacacct ataaaagagt gagccgttat
     3481 cgtctgtttg tggacgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc
     3541 cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg
     3601 catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc
     3661 gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt
     3721 aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt
     3781 cgaccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa
     3841 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta
     3901 caaagtggtt gatcgcgtgc atgcgacgtc atagctctct ccctatagtg agtcgtatta
     3961 taagctaggc actggccgtc gttttacaac gtcgtgactg ggaaaactgc tagcttggga
     4021 tctttgtgag ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
     4081 tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctagctgcat
     4141 atgcttgctg cttgagagtt ttgcttactg agtatgattt atgaaaatat tatacacagg
     4201 agctagtgat tctaattgtt tgtgtatttt agattcacag tcccaaggct catttcaggc
     4261 ccctcagtcc tcacagtctg ttcatgatca taatcagcca taccacattt gtagaggttt
     4321 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataag atgaatgcaa
     4381 ttgttgttgt taacttgttt attgcagctt acaatggtta caaataaagc aatagcatca
     4441 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     4501 tcaatgtatc ttatcatgtc tggatcgatc ccgcattaat gaatcggcca acgcgcgggg
     4561 agaggcggtt tgcgtattgg ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     4621 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     4681 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4741 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4801 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4861 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4921 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4981 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5041 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     5101 acaatttcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     5161 gcggatctgc gcagcaccat ggcctgaaat aacctctgaa agaggaactt ggttaggtac
     5221 cttctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     5281 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     5341 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     5401 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     5461 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     5521 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     5581 aaagcttgat tcttctgaca caacagtctc gaacttaagg ctagagccac catgattgaa
     5641 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     5701 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     5761 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     5821 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     5881 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     5941 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     6001 catacgcttg atccggctac ctgcccattc gaccaccaag cgaagcatcg catcgagcga
     6061 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     6121 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     6181 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     6241 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     6301 gctacccgtg atattgctgc agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     6361 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     6421 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     6481 gatggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa
     6541 tcgatagcga taaggatccg cgtatggtgc actctcagta caatctgctc tgatgccgca
     6601 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     6661 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     6721 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     6781 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     6841 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     6901 agacaataac cctgataaat gcttcaataa tattaaaaaa ggaagagtat gagtattcaa
     6961 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     7021 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     7081 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     7141 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     7201 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     7261 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     7321 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     7381 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     7441 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     7501 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     7561 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     7621 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     7681 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     7741 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     7801 cattggtaac tgtcaggcca agtttactca tatatacttt agattgattt aaaacctcat
     7861 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     7921 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//
