LOCUS       sequence-321701-        3128 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid carries Yn situ preamplifier A1-20B1 sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Wu Y, Xu W, Ma L, Yu Z, Wang Y, Yu CR
  TITLE     Robust and sensitive in situ RNA detection using Yn-situ.
  JOURNAL   Cell Rep Methods. 2022 Apr 18;2(4):100201. doi: 
            10.1016/j.crmeth.2022.100201. eCollection 2022 Apr 25.
  PUBMED    35497500
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2022-04-18"; volume: "2(4):100201. doi"; pages: " 
            10.1016/j.crmeth.2022.100201. eCollection 2022 Apr 25"
COMMENT     Sequence Label: A1-20B1 (P001)
FEATURES             Location/Qualifiers
     source          1..3128
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             24..35
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1015..1032)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1125..1713)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1205..1224)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1887..2681)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2682..2786)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3087..3109
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3101..3118
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3102..3118
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taatacgact cactataggg cgaattgaag gaaggccgtc aaggccgcat ggagcatgcg
       61 acgtcgggcc caattcgccc gcttgtcgtt gacgtgacgt atgattctct aagaggaggg
      121 cagcaaacgg gaagagtctt cctttacgaa gaggagggca gcaaacggga agagtcttcc
      181 tttacgaaga ggagggcagc aaacgggaag agtcttcctt tacgaagagg agggcagcaa
      241 acgggaagag tcttccttta cgaagaggag ggcagcaaac gggaagagtc ttcctttacg
      301 aagaggaggg cagcaaacgg gaagagtctt cctttacgaa gaggagggca gcaaacggga
      361 agagtcttcc tttacgaaga ggagggcagc aaacgggaag agtcttcctt tacgaagagg
      421 agggcagcaa acgggaagag tcttccttta cgaagaggag ggcagcaaac gggaagagtc
      481 ttcctttacg aagaggaggg cagcaaacgg gaagagtctt cctttacgaa gaggagggca
      541 gcaaacggga agagtcttcc tttacgaaga ggagggcagc aaacgggaag agtcttcctt
      601 tacgaagagg agggcagcaa acgggaagag tcttccttta cgaagaggag ggcagcaaac
      661 gggaagagtc ttcctttacg aagaggaggg cagcaaacgg gaagagtctt cctttacgaa
      721 gaggagggca gcaaacggga agagtcttcc tttacgaaga ggagggcagc aaacgggaag
      781 agtcttcctt tacgaagagg agggcagcaa acgggaagag tcttccttta cgaagaggag
      841 ggcagcaaac gggaagagtc ttcctttacg tatagtgagt cgtattacaa ttcactggcc
      901 ctgggcctca tgggccttcc tttcactgcc cgctttccag tcgggaaacc tgtcgtgcca
      961 gctgcattaa catggtcata gctgtttcct tgcgtattgg gcgctctccg cttcctcgct
     1021 cactgactcg ctgcgctcgg tcgttcgggt aaagcctggg gtgcctaatg agcaaaaggc
     1081 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     1141 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1201 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1261 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1321 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1381 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1441 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1501 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1561 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1621 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1681 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1741 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     1801 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     1861 tatgagtaaa cttggtctga cagttattag aaaaattcat ccagcagacg ataaaacgca
     1921 atacgctggc tatccggtgc cgcaatgcca tacagcacca gaaaacgatc cgcccattcg
     1981 ccgcccagtt cttccgcaat atcacgggtg gccagcgcaa tatcctgata acgatccgcc
     2041 acgcccagac ggccgcaatc aataaagccg ctaaaacggc cattttccac cataatgttc
     2101 ggcaggcacg catcaccatg ggtcaccacc agatcttcgc catccggcat gctcgctttc
     2161 agacgcgcaa acagctctgc cggtgccagg ccctgatgtt cttcatccag atcatcctga
     2221 tccaccaggc ccgcttccat acgggtacgc gcacgttcaa tacgatgttt cgcctgatga
     2281 tcaaacggac aggtcgccgg gtccagggta tgcagacgac gcatggcatc cgccataatg
     2341 ctcacttttt ctgccggcgc cagatggcta gacagcagat cctgacccgg cacttcgccc
     2401 agcagcagcc aatcacggcc cgcttcggtc accacatcca gcaccgccgc acacggaaca
     2461 ccggtggtgg ccagccagct cagacgcgcc gcttcatcct gcagctcgtt cagcgcaccg
     2521 ctcagatcgg ttttcacaaa cagcaccgga cgaccctgcg cgctcagacg aaacaccgcc
     2581 gcatcagagc agccaatggt ctgctgcgcc caatcatagc caaacagacg ttccacccac
     2641 gctgccgggc tacccgcatg caggccatcc tgttcaatca tactcttcct ttttcaatat
     2701 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     2761 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
     2821 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     2881 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     2941 gtggccgcta cagggcgctc ccattcgcca ttcaggctgc gcaactgttg ggaagggcgt
     3001 ttcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3061 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3121 gcgcgacg
//
