LOCUS       sequence-321879-        7402 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Nup93 fused at its carboxyl-terminus to 
            mEGFP with no linker and a total net fusion of (-5 amino acids) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7402)
  TITLE     Simon lab plasmids
REFERENCE   2  (bases 1 to 7402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Nup93_mEGFP(-5)
FEATURES             Location/Qualifiers
     source          1..7402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(439..460)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     788..807
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1047..1068
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(3660..3863)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3689..3709)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3864..4167
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(4342..4930)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4422..4441)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5259..5306
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5331..5350
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5538..6332)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(5538..6332)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5649..5668)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6259..6278
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6367..6724)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6381..6516
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        6533..6724
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6681..6701
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(6726..6830)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6857..7312
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6944..6963)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7154..7175
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    join(7319..7402,1..38)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7330..7349)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     join(7384..7402,1)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
       61 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      121 aatgtggtat ggctgattat gatcagttat ctagatccgg tggatctccg cggagctcgg
      181 atcctcgagg gatactctag agcggccgcc cntttttttt tttttttttt ttttttttac
      241 aaatcaaaga aatttattgg cgttgacaaa aatacataat acagaaacca aaacaaaaca
      301 caacagaaac aaaaccagaa accccagcct agtgggccca tgtgcctgat gtactgacag
      361 tgtgccgacc cagactccca caaagcatgg cacttacttg tacagctcgt ccatgccgag
      421 agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg
      481 gtctttgctc agggcggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc
      541 gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat
      601 gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata
      661 gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt
      721 gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc
      781 ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc
      841 ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa
      901 gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc
      961 ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc
     1021 ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac
     1081 cccggtgaac agctcctcat tcatgaggac ctccatctgc accagcctcg cattggtgtc
     1141 cccagacgtt cggtatggta tcattccagc aaaggtaatc agagtgcggg cttgacttcg
     1201 gagttgagag tcgcggtcct cgatgactcg ctggggcctg gatgacgagg atggacttgt
     1261 ccccttgagc ctcttaaact gtgtgaacaa gatgttcatg gtggcaagaa gcacttctga
     1321 gaggttgtgc ctgatttcat cactgaaatt tctgaaggca gccactctct cttccacact
     1381 ttcctgattc aggggcacca gcttcaagcg ctcaatgata tcaaaagctc tatcaatatg
     1441 accactatga tactcgtcaa aaaaggtgat caagtccaaa agaagataga acgtggagtc
     1501 cacaaattta tttgcgctta ttccttgagc cctataccgt tcggcaatgg agagtgccat
     1561 gttcttcagc ctctccttgt tggattgcgg ggcactgatc tgggggacga cagggctaag
     1621 cagtttgttc atcagctcca gtaccttgtc agcattcttg gcaaggtcat acagctttgc
     1681 tgcctcttca aacagtcctt tattttctgc cacagaagca actttgttga taataggctt
     1741 tgtgtcacta gtaaacttat ctatgactcc aggctttcta cttccgtcat tctctagttt
     1801 cccaagaatc atatcgaact ctcggctttc aatcacaagc tcactcacac agcgcagaaa
     1861 catgttttct ccttgactat ctttctcatc cctgaggaaa tagaagtact ggagggcctc
     1921 ccttgggtcc gtggactcaa acttccgggt gtacagcatg aggagccgca cgaagttcag
     1981 ccgccgcaag caaggagggt caccaggctc gtggctgagg agctgagccc tctgtccaga
     2041 ggactttaaa agcagcttca gctcaaacag caccagtgct acatggacag catggcagcg
     2101 cagccgctcc atgcggaaaa gaaaggcaac tgctgcttca aactgcgctg tcaggaacag
     2161 gacttggaag tagaggaagg gttgctggtt caccgtaaag tgggactcgc catagtcttc
     2221 caacaactgc ttctggaact gtgagagagt gagcctgtct tgtggggagc tggtgccatc
     2281 gtcgtcaaaa cacacttggt tcaacttcag ccacaggtaa tcctcagttt tgtccgccac
     2341 ttcactctgg ttgtcggtga cgtcacatct gccaatgata cagtacacgg cccgcttgta
     2401 gggatctgta ttgttcctga gggccctacg gtaatgcagc cggagcttgt tttccgtagc
     2461 tggggacaat cttctgtcct tgctgttcat gtactcctgg aaccaggttt taaactctcc
     2521 cagctggtgc tgggctcgat taactacctg tgaagcggca agcaggtctc cacagcgcat
     2581 gcagtagtaa attagcgccc acacaggatg gccttccacc tctccatcct gtagtccagg
     2641 caagggagct ggcagtttaa tgttcaggaa acttcgaacc aattggtaag tcccaggcac
     2701 cccgcccagc tgggcctgat gcaaatttcc aaagacagtc acaagggtgt aattcttata
     2761 actctgctca aggtacgcca aggcctgcct gacaaactcc atgcgcactt ccacgctgct
     2821 gcggttcttc agggcatccg ttgccggtgt caacaacacg tctgtcattt gttttaccat
     2881 ggtccacatg tcggaaatgc tcttatcatc gagctctgcg acggaagcac aaaggtccac
     2941 caggttaggc tgcaggtgtc catttacaat tttctcatta tagatataaa tttgccgcgc
     3001 ataggccatc tcgatgttat ccagagagct tcgaccaggg ggtcccacat cactgatgta
     3061 gcttggctcg ctttcttgag taaagtcaag ggcgtcttct cctgatgcca gcagtgtgtg
     3121 cagaattcgc tgtttcactt gctcccactc aaccaacatt gactcccgat ggtactcctc
     3181 agccatgccg aaggtcctct tccgggactc ttcgatggca gacagcaggg cattgtcctt
     3241 ctcattcttc aggaagccct gaatgtcagt gtccttcaca ggctcaagag gctcaaaggt
     3301 ggtggctgca ctcagactct ccaatcgctg ggagatgtgg gatatgtcaa gtccccgaga
     3361 cccgaggaga actgacgcct tgacatctgc cgtctcctgg gacgtgcgtg ttagggtacg
     3421 ggaacgcagg cgctctcccg cctgctggat ctcctgtaag ttccgttcca catggggaag
     3481 ctctgagatg ccctcagtct cagcagcaag ctgttcagct tgctgaagga gctcaccaaa
     3541 cccctcagta tccattggag atgcagatcc ttgtgctaca gccgtcatcc cgggaagtcg
     3601 agatctgagt ccggaggtgg cgaccggtag cgctagcgga tctgacggtt cactaaacca
     3661 gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg
     3721 gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg
     3781 acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg
     3841 atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc
     3901 caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg
     3961 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg
     4021 gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact
     4081 atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc
     4141 gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac
     4201 cccgtaattg attactatta ataactaatg catggcggta atacggttat ccacagaatc
     4261 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     4321 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     4381 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     4441 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     4501 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     4561 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     4621 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     4681 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     4741 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     4801 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     4861 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     4921 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     4981 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     5041 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaacctg aggctatggc
     5101 agggcctgcc gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttggggggtg
     5161 gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg gtggggtatc
     5221 gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc caacaccgtg
     5281 cgttttattc tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt
     5341 ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac tccagcatga gatccccgcg
     5401 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa
     5461 ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc
     5521 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc
     5581 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc
     5641 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc
     5701 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag
     5761 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg
     5821 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga
     5881 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg
     5941 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc
     6001 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc
     6061 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg
     6121 gccagccacg atagccgcgc tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg
     6181 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag
     6241 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga
     6301 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga
     6361 tcgatctttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
     6421 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg
     6481 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat
     6541 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
     6601 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
     6661 ggagcctggg gactttccac accctaactg acacacattc cacagctggt tctttccgcc
     6721 tcaggactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     6781 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     6841 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     6901 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     6961 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     7021 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     7081 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     7141 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     7201 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     7261 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttacgcgtta
     7321 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     7381 tgtgaaattt gtgatgctat tg
//
