LOCUS       sequence-321908-       10589 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral overexpression vector for human HK2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10589)
  AUTHORS   Rossiter NJ, Huggler KS, Adelmann CH, Keys HR, Soens RW, Sabatini 
            DM, Cantor JR
  TITLE     CRISPR screens in physiologic medium reveal conditionally essential 
            genes in human cells.
  JOURNAL   Cell Metab. 2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 
            10.1016/j.cmet.2021.02.005.
  PUBMED    33651980
REFERENCE   2  (bases 1 to 10589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Metab.
            2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 
            10.1016/j.cmet.2021.02.005."
COMMENT     Sequence Label: pLJC6-HK2-3xFLAG
FEATURES             Location/Qualifiers
     source          1..10589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     114..133
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     367..384
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    501..522
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(537..542,543..560,561..567)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    575..591
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     580..602
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     599..615
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     599..615
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     634..654
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        636..654
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        682..908
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             909..1089
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1136..1261
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1754..1987
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2172..2216
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2505..3716
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3553..3572
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     RBS             6208..6216
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6524..6589
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    6637..6754
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6803..7313
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7232..7250
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7322..7717
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             7789..8022
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8094..8215
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8131..8150)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8185..8204
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8255..8390
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8410..8429)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8411..8429)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8439..8456)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8439..8455)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8448..8470)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8597..9052
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8684..8703)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8894..8915
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9078..9182
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9183..9251,9252..10043)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      join(10214..10589,1..213)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
       61 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
      121 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
      181 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
      241 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
      301 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
      361 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
      421 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
      481 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
      541 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
      601 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
      661 aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg
      721 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
      781 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
      841 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
      901 acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
      961 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     1021 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     1081 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     1141 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     1201 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     1261 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     1321 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     1381 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     1441 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     1501 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     1561 ggaagagcaa aacaaaagta agaccaccgc acagcaagca gcagctgatc ttcagacctg
     1621 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1681 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1741 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1801 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1861 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1921 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1981 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     2041 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     2101 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     2161 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     2221 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     2281 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     2341 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     2401 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     2461 gatctcgacg gtatcgatca cgagactagc ctcgagcggg atctggcctc cgcgccgggt
     2521 tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt cagacgaagg
     2581 gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc gctgctcata
     2641 agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg acttgggtga
     2701 ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt agtcccttct
     2761 cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat ataaggacgc
     2821 gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt
     2881 ggatcgctgt gatcgtcact tggtgagtag cgggctgctg ggctggccgg ggctttcgtg
     2941 gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg
     3001 ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcagcaa aatggcggct
     3061 gttcccgagt cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg
     3121 tggggggcat ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag
     3181 ctcttattcg ggtgagatgg gctggggcac catctgggga ccctgacgtg aagtttgtca
     3241 ctgactggag aactcggttt gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg
     3301 ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc
     3361 tgttggctta taatgcaggg tggggccacc tgccggtagg tgtgcggtag gcttttctcc
     3421 gtcgcaggac gcagggttcg ggcctagggt aggctctcct gaatcgacag gcgccggacc
     3481 tctggtgagg ggagggataa gtgaggcgtc agtttctttg gtcggtttta tgtacctatc
     3541 ttcttaagta gctgaagctc cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt
     3601 gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt aattttcagt
     3661 gttagactag taaattgtcc gctaaattct ggccgttttt ggcttttttg ttagacgaag
     3721 cgctagcgct accggtttaa ttaacggcag gatgattgcc tcgcatctgc ttgcctactt
     3781 cttcacggag ctcaaccatg accaagtgca gaaggttgac cagtatctct accacatgcg
     3841 cctctctgat gagaccctct tggagatctc caaacgcttt cggaaagaaa tggagaaagg
     3901 gcttggagcc accactcacc ctactgcagc agtgaagatg ctgcccacct ttgtgaggtc
     3961 cactccagat gggacagaac acggagagtt cctggctctg gatcttggag ggaccaactt
     4021 ccgtgtgctt tgggtgaaag taacggacaa tgggctccag aaggtggaga tggagaatca
     4081 gatctatgcc atccctgagg acatcatgcg aggcagtggc acccagctgt ttgaccacat
     4141 tgccgaatgc ctggctaact tcatggataa gctacaaatc aaagacaaga agctcccact
     4201 gggttttacc ttctcgttcc cctgccacca gactaaacta gacgagagtt tcctggtctc
     4261 atggaccaag ggattcaagt ccagtggagt ggaaggcaga gacgttgtgg ctctgatccg
     4321 gaaggccatc cagaggagag gggactttga tatcgacatt gtggctgtgg tgaatgacac
     4381 agttgggacc atgatgacct gtggttatga tgaccacaac tgtgagattg gtctcattgt
     4441 gggcacgggc agcaacgcct gctacatgga agagatgcgc cacatcgaca tggtggaagg
     4501 cgatgagggg cggatgtgca tcaatatgga gtggggggcc ttcggggacg atggctcgct
     4561 caacgacatt cgcactgagt ttgaccagga gattgacatg ggctcactga acccgggaaa
     4621 gcaactgttt gagaagatga tcagtgggat gtacatgggg gagctggtga ggcttatcct
     4681 ggtgaagatg gccaaggagg agctgctctt tggggggaag ctcagcccag agcttctcaa
     4741 caccggtcgc tttgagacca aagacatctc agacattgaa ggggagaagg atggcatccg
     4801 gaaggcccgt gaggtcctga tgcggttggg cctggacccg actcaggagg actgcgtggc
     4861 cactcaccgg atctgccaga tcgtgtccac acgctccgcc agcctgtgcg cagccaccct
     4921 ggccgccgtg ctgcagcgca tcaaggagaa caaaggcgag gagcggctgc gctctactat
     4981 tggggtcgac ggttccgtct acaagaaaca cccccatttt gccaagcgtc tacataagac
     5041 cgtgcggcgg ctggtgcccg gctgcgatgt ccgcttcctc cgctccgagg atggcagtgg
     5101 caaaggtgca gccatggtga cagcagtggc ttaccggctg gccgatcaac accgtgcccg
     5161 ccagaagaca ttagagcatc tgcagctgag ccatgaccag ctgctggagg tcaagaggag
     5221 gatgaaggta gaaatggagc gaggtctgag caaggagact catgccagtg cccccgtcaa
     5281 gatgctgccc acctacgtgt gtgctacccc ggacggcaca gagaaagggg acttcttggc
     5341 cttggacctt ggaggaacaa atttccgggt cctgctggtc cgtgttcgga atgggaagtg
     5401 gggtggagtg gagatgcaca acaagatcta cgccatcccg caggaggtca tgcacggcac
     5461 cggggacgag ctctttgacc acattgtcca gtgcatcgcg gacttcctcg agtacatggg
     5521 catgaagggc gtgtccctgc ctctgggttt taccttctcc ttcccctgcc agcagaacag
     5581 cctggacgag agcatcctcc tcaagtggac aaaaggcttc aaggcatctg gctgcgaggg
     5641 cgaggacgtg gtgaccctgc tgaaggaagc gatccaccgg cgagaggagt ttgacctgga
     5701 tgtggttgct gtggtgaacg acacagtcgg aactatgatg acctgtggct ttgaagaccc
     5761 tcactgtgaa gttggcctca ttgttggcac gggcagcaat gcctgctaca tggaggagat
     5821 gcgcaatgtg gaactggtgg aaggagaaga ggggcggatg tgtgtgaaca tggaatgggg
     5881 ggccttcggg gacaatggat gcctagatga cttccgcaca gaatttgatg tggctgtgga
     5941 tgagctttca ctcaacccgg gcaagcagag gttcgagaaa atgatcagtg gaatgtacct
     6001 gggtgagatt gtccgtaaca ttctcatcga tttcaccaag cgtggactac tcttccgagg
     6061 ccgcatctca gagcggctca agacaagggg catctttgaa accaagttct tgtctcagat
     6121 tgagagtgac tgcctggccc tgctgcaagt ccgagccatc ctgcaacact tagggcttga
     6181 gagcacctgt gacgacagca tcattgttaa ggaggtgtgc actgtggtgg cccggcgggc
     6241 agcccagctc tgtggcgcag gcatggccgc tgtggtggac aggatacgag aaaaccgtgg
     6301 gctggacgct ctcaaagtga cagtgggtgt ggatgggacc ctctacaagc tacatcctca
     6361 ctttgccaaa gtcatgcatg agacagtgaa ggacctggct ccgaaatgtg atgtgtcttt
     6421 cctgcagtca gaggatggca gcgggaaggg ggcggcgctc atcactgctg tggcctgccg
     6481 catccgtgag gctggacagc gaggaggaag tgcggccgca ggcgactaca aagaccatga
     6541 cggtgattat aaagatcatg acattgatta caaggatgac gatgacaagt aataagaatt
     6601 ctcgacctcg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     6661 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     6721 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     6781 tccactttgg ccgcggctcg agggggttgg ggttgcgcct tttccaaggc agccctgggt
     6841 ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg
     6901 ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg
     6961 tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg
     7021 cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac
     7081 agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct
     7141 cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg
     7201 cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc
     7261 gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagggggatc
     7321 catggttcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa
     7381 cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg
     7441 catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt
     7501 gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa
     7561 tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca
     7621 tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg
     7681 tgaattgctg ccctctggtt atgtgtggga gggctaaggt acctttaaga ccaatgactt
     7741 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     7801 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     7861 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7921 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7981 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     8041 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     8101 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     8161 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     8221 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     8281 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8341 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     8401 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     8461 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     8521 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     8581 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     8641 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     8701 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     8761 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     8821 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     8881 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     8941 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     9001 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     9061 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     9121 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     9181 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     9241 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     9301 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     9361 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     9421 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     9481 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     9541 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     9601 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     9661 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     9721 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     9781 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     9841 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     9901 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     9961 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    10021 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    10081 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    10141 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    10201 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    10261 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    10321 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    10381 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    10441 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    10501 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    10561 tggagcgaac gacctacacc gaactgaga
//
