LOCUS       sequence-321948-       13183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium
            tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum 
            and recAR61C allele..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13183)
  AUTHORS   Yan MY, Li SS, Ding XY, Guo XP, Jin Q, Sun YC
  TITLE     A CRISPR-Assisted Nonhomologous End-Joining Strategy for Efficient 
            Genome Editing in Mycobacterium tuberculosis.
  JOURNAL   mBio. 2020 Jan 28;11(1). pii: mBio.02364-19. doi: 
            10.1128/mBio.02364-19.
  PUBMED    31992616
REFERENCE   2  (bases 1 to 13183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2020-01-28"; volume: "11(1). pii: mBio.02364-19. doi"; pages:
            " 10.1128/mBio.02364-19"
COMMENT     Sequence Label: pNHEJ-recAmu-sacB
FEATURES             Location/Qualifiers
     source          1..13183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(92..111)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             103..918
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(176..195)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        1206..1651
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(1652..1738,1739..3073)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFVKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     rep_origin      3161..3749
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3650..3669
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             9629..9640
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gttgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca
       61 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
      121 gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
      181 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag
      241 ccccatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
      301 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
      361 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg gaaaacagca
      421 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
      481 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
      541 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
      601 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taatcttttg
      661 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
      721 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
      781 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
      841 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
      901 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg
      961 ctgacttgac gggacggcgg ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc
     1021 agatcacgca tcttcccgac aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca
     1081 ccaactggtc cacctacaac aaagctctca ccaaccgtgg ctccctcact ttctggctgg
     1141 atgatggggc gattcaggcc tggtatgagt cagcaacacc ttcttcacga ggcagacctc
     1201 accatcacat atacctgccg ttcactatta tttagtgaaa tgagatatta tgatattttc
     1261 tgaattgtga ttaaaaaggc aactttatgc ccatgcaaca gaaactataa aaaatacaga
     1321 gaatgaaaag aaacagatag attttttagt tctttaggcc cgtagtctgc aaatcctttt
     1381 atgattttct atcaaacaaa agaggaaaat agaccagttg caatccaaac gagagtctaa
     1441 tagaatgagg tcgaaaagta aatcgcgcgg gtttgttact gataaagcag gcaagaccta
     1501 aaatgtgtaa agggcaaagt gtatactttg gcgtcacccc ttacatattt taggtctttt
     1561 tttattgtgc gtaactaact tgccatcttc aaacaggagg gctggaagaa gcagaccgct
     1621 aacacagtac ataaaaaagg agacatgaac gatgaacatc aaaaagtttg taaaacaagc
     1681 aacagtatta acctttacta ccgcactgct ggcaggaggc gcaactcaag cgtttgcgaa
     1741 agaaacgaac caaaagccat ataaggaaac atacggcatt tcccatatta cacgccatga
     1801 tatgctgcaa atccctgaac agcaaaaaaa tgaaaaatat caagttcctg agttcgattc
     1861 gtccacaatt aaaaatatct cttctgcaaa aggcctggac gtttgggaca gctggccatt
     1921 acaaaacgct gacggcactg tcgcaaacta tcacggctac cacatcgtct ttgcattagc
     1981 cggagatcct aaaaatgcgg atgacacatc gatttacatg ttctatcaaa aagtcggcga
     2041 aacttctatt gacagctgga aaaacgctgg ccgcgtcttt aaagacagcg acaaattcga
     2101 tgcaaatgat tctatcctaa aagaccaaac acaagaatgg tcaggttcag ccacatttac
     2161 atctgacgga aaaatccgtt tattctacac tgatttctcc ggtaaacatt acggcaaaca
     2221 aacactgaca actgcacaag ttaacgtatc agcatcagac agctctttga acatcaacgg
     2281 tgtagaggat tataaatcaa tctttgacgg tgacggaaaa acgtatcaaa atgtacagca
     2341 gttcatcgat gaaggcaact acagctcagg cgacaaccat acgctgagag atcctcacta
     2401 cgtagaagat aaaggccaca aatacttagt atttgaagca aacactggaa ctgaagatgg
     2461 ctaccaaggc gaagaatctt tatttaacaa agcatactat ggcaaaagca catcattctt
     2521 ccgtcaagaa agtcaaaaac ttctgcaaag cgataaaaaa cgcacggctg agttagcaaa
     2581 cggcgctctc ggtatgattg agctaaacga tgattacaca ctgaaaaaag tgatgaaacc
     2641 gctgattgca tctaacacag taacagatga aattgaacgc gcgaacgtct ttaaaatgaa
     2701 cggcaaatgg tacctgttca ctgactcccg cggatcaaaa atgacgattg acggcattac
     2761 gtctaacgat atttacatgc ttggttatgt ttctaattct ttaactggcc catacaagcc
     2821 gctgaacaaa actggccttg tgttaaaaat ggatcttgat cctaacgatg taacctttac
     2881 ttactcacac ttcgctgtac ctcaagcgaa aggaaacaat gtcgtgatta caagctatat
     2941 gacaaacaga ggattctacg cagacaaaca atcaacgttt gcgccaagct tcctgctgaa
     3001 catcaaaggc aagaaaacat ctgttgtcaa agacagcatc cttgaacaag gacaattaac
     3061 agttaacaaa taaaaacgca aaagaaaatg ccgatattga ctaccggaag cagtgtgttc
     3121 catgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3181 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3241 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3301 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3361 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3421 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3481 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3541 tacagcgtga gctatgagaa agcgccacgc ttcccgaggg gagaaaggcg gacaggtatc
     3601 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3661 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3721 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3781 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3841 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3901 gcaacgcgtg agcccaccag ctccgtaagt tcgggcgctg tgtggctcgt acccgcgcat
     3961 tcaggcggca gggggtctaa cgggtctaag gcggcgtgta cggccgccac agcggctctc
     4021 agcggcccgg aaacgtcctc gaaacgacgc atgtgttcct cctggttggt acaggtggtt
     4081 gggggtgctc ggctgtcgct ggtgttccac caccagggct cgacgggaga gcgggggagt
     4141 gtgcagttgt ggggtggccc ctcagcgaaa tatctgactt ggagctcgtg tcggaccata
     4201 caccggtgat taatcgtggt ctactaccaa gcgtgagcca cgtcgccgac gaatttgagc
     4261 agctctggct gccgtactgg ccgctggcaa gcgacgatct gctcgagggg atctaccgcc
     4321 aaagccgcgc gtcggcccta ggccgccggt acatcgaggc gaacccaaca gcgctggcaa
     4381 acctgctggt cgtggacgta gaccatccag acgcagcgct ccgagcgctc agcgcccggg
     4441 ggtcccatcc gctgcccaac gcgatcgtgg gcaatcgcgc caacggccac gcacacgcag
     4501 tgtgggcact caacgcccct gttccacgca ccgaatacgc gcggcgtaag ccgctcgcat
     4561 acatggcggc gtgcgccgaa ggccttcggc gcgccgtcga tggcgaccgc agttactcag
     4621 gcctcatgac caaaaacccc ggccacatcg cctgggaaac ggaatggctc cactcagatc
     4681 tctacacact cagccacatc gaggccgagc tcggcgcgaa catgccaccg ccgcgctggc
     4741 gtcagcagac cacgtacaaa gcggctccga cgccgctagg gcggaattgc gcactgttcg
     4801 attccgtcag gttgtgggcc tatcgtcccg ccctcatgcg gatctacctg ccgacccgga
     4861 acgtggacgg actcggccgc gcgatctatg ccgagtgcca cgcgcgaaac gccgaatttc
     4921 cgtgcaacga cgtgtgtccc ggaccgctac cggacagcga ggtccgcgcc atcgccaaca
     4981 gcatttggcg ttggatcaca accaagtcgc gcatttgggc ggacgggatc gtggtctacg
     5041 aggccacact cagtgcgcgc cagtcggcca tctcgcggaa gggcgcagca gcgcgcacgg
     5101 cggcgagcac agttgcgcgg cgcgcaaagt ccgcgtcagc catggaggca ttgctatgag
     5161 cgacggctac agcgacggct acaaccggca gccgactgtc cgcaaaaagc ggcgcgtgac
     5221 cgccgccgaa ggcgctcgaa tcaccggact atccgaacgc cacgtcgtcc ggctcgtggc
     5281 gcaggaacgc agcgagtggc tcgccgagca ggctgcacgc cgcgaacgca tccgcgccta
     5341 tcacgacgac gagggccact cttggccgca aacggccaaa catttcgggc tgcatctgga
     5401 caccgttaag cgactcggct atcgggcgag gaaagagcgt gcggcagaac aggaagcggc
     5461 tcaaaaggcc cacaacgaag ccgacaatcc accgctgttc taacgcaatt ggggagcggg
     5521 tgtcgcgggg gttccgtggg gggttccgtt gcaacgggtc ggacaggtaa aagtcctggt
     5581 agacgctagt tttctggttt gggccatgcc tgtctcgttg cgtgtttcgt tgcgcccgtt
     5641 ttgaatacca gccagacgag acggggttct acgaatcttg gtcgatacca agccatttcc
     5701 gctgaatatc ggggagctca ccgccagaat cggtggttgt ggtgatgtac gtggcgaact
     5761 ccgttgtagt gcctgtggtg gcatccgtgg cgcggccgcc aggctagcgg tgttgagcag
     5821 atcgtcggtg atccggacca gccgcgcacg caagtcgggc cgcaccgccg ccagggcgtt
     5881 cgacgcgccg acgagcgcgg acgcgatgtt gccacacgcg gcgtgtcaca cttgaatcga
     5941 acaggtgttc ggctactgtg gtgatcattc ggagcagccg acttgtcagt ggctgtctct
     6001 agtgtcacgg ccaaccgacc gataccggtc aatcgaacac cgaccacagg agaggcacca
     6061 tgacgcagac ccccgatcgg gaaaaggcgc tcgagctggc agtggcccag atcgagaaga
     6121 gttacggcaa aggttcggtg atgcgcctcg gcgacgaggc gcgtcagccg atttcggtca
     6181 ttccgaccgg atccatcgca ctagacgtgg ccctgggcat tggcggcctg ccgcgtggct
     6241 gcgtgataga gatatacggc ccggagtcgt cgggtaagac caccgtggcg ctgcacgcgg
     6301 tggccaacgc tcaggccgcc ggtggtgttg cggcgttcat cgacgccgag cacgcgctgg
     6361 atccggacta tgccaagaag ctcggtgtcg acaccgattc gctgctggtc agccagccgg
     6421 acaccgggga acaggcactc gagatcgccg acatgctgat ccgctcgggt gcgcttgaca
     6481 tcgtggtgat cgactcggtg gcggcgctgg tgccgcgcgc ggagctcgaa ggcgagatgg
     6541 gcgacagcca cgtcgggctg caggcccggc tgatgagcca ggcgctgcgg aaaatgaccg
     6601 gcgcgctgaa taattcgggc accacggcga tcttcatcaa ccagctccgc gacaagatcg
     6661 gagtgatgtt cgggtcgccc gagacgacaa cgggcggaaa ggcgttgaag ttctacgcgt
     6721 cggtgcgcat ggacgtgcgg cgagtcgaga cgctcaagga cggtaccaac gcggtcggca
     6781 accgcacccg ggtcaaggtc gtcaagaaca agtgcctcgc agagggcact cggatcttcg
     6841 atccggtcac cggtacaacg catcgcatcg aggatgttgt cgatgggcgc aagcctattc
     6901 atgtcgtggc tgctgccaag gacggaacgc tgcatgcgcg gcccgtggtg tcctggttcg
     6961 accagggaac gcgggatgtg atcgggttgc ggatcgccgg tggcgccatc gtgtgggcga
     7021 cacccgatca caaggtgctg acagagtacg gctggcgtgc cgccggggaa ctccgcaagg
     7081 gagacagggt ggcgcaaccg cgacgcttcg atggattcgg tgacagtgcg ccgattccgg
     7141 cggatcatgc ccggctgctt ggctacctga tcggagatgg cagggatggt tgggtggggg
     7201 gcaagactcc gatcaacttc atcaatgttc agcgggcgct cattgacgac gtgacgcgaa
     7261 tcgctgcgac gctcggttgc gcggcccatc cgcaggggcg tatctcactc gcgatcgctc
     7321 atcgacccgg tgagcgcaac ggtgtggcag acctttgtca gcaggccggt atctacggca
     7381 agctcgcgtg ggagaagacg attccgaatt ggttcttcga gccggacatc gcggccgaca
     7441 ttgtcggcaa tctgctcttc ggcctgttcg aaagcgacgg gtgggtgagc cgggaacaga
     7501 ccggggcact tcgggtcggt tacacgacga cctctgaaca actcgcgcat cagattcatt
     7561 ggctgctgct gcggttcggt gtcgggagca ccgttcgaga ttacgatccg acccagaagc
     7621 ggccgagcat cgtcaacggt cgacggatcc agagcaaacg tcaagtgttc gaggtccgga
     7681 tctcgggtat ggataacgtc acggcattcg cggagtcagt tcccatgtgg gggccgcgcg
     7741 gtgccgcgct tatccaggcg attccagaag ccacgcaggg gcggcgtcgt ggatcgcaag
     7801 cgacatatct ggctgcagag atgaccgatg ccgtgctgaa ttatctggac gagcgcggcg
     7861 tgaccgcgca ggaggccgcg gccatgatcg gtgtagcttc cggggacccc cgcggtggaa
     7921 tgaagcaggt cttaggtgcc agccgccttc gtcgggatcg cgtgcaggcg ctcgcggatg
     7981 ccctggatga caaattcctg cacgacatgc tggcggaaga actccgctat tccgtgatcc
     8041 gagaagtgct gccaacgcgg cgggcacgaa cgttcgacct cgaggtcgag gaactgcaca
     8101 ccctcgtcgc cgaaggggtt gtcgtgcaca actgttcgcc ccccttcaag caggccgagt
     8161 tcgacatcct ctacggcaag ggaatcagca gggagggctc gctgatcgac atgggtgtgg
     8221 atcagggcct catccgcaag tcgggtgcct ggttcaccta cgagggcgag cagctcggcc
     8281 agggcaagga gaatgcccgc aacttcttgg tggagaacgc cgacgtggct gacgagatcg
     8341 agaagaagat caaggaaaag cttggcattg gtgccgtggt gaccgatgat ccctcaaatg
     8401 acggtgtcct gcccgccccc gtcgacttct gagcgcgaag agcaggcgcg ggcactggcg
     8461 gccgcggtac ccaagaagca cagtgagtcg cggatcgatg gcgggcgcgg tatcggcgcc
     8521 gccaacgtca cctcgtcgag agccaccacc tcactcggtg agcgcaagac gttttcaccg
     8581 gcttcgcgta atccgtcggc gccgcgcttg atcaaatcga gcagggcgac tcccggcgcc
     8641 atggcgtaga tcgaaccgtc ggacaacacc ccgacacgct caccgtccgc acttcgatac
     8701 gtcacccatt tcattcgcgc accacctcac tcgggttttt gtccgggcgc aacccacgcc
     8761 agctggattg ccgcaatcgg tcatcggggg tccactcgct atagcgcacc tccccgacca
     8821 gcgtcggcgc gacgaacgta acacccttgg cctcgcgcgc cggaagcgca gcgtcgaatg
     8881 gggattcgtc ggtatgcagc ggcgccagcg tcttcttgag gttggccagg tcacgttcgg
     8941 tgaagccggt tccaacacgg ccggcgaagt gcaaaccgct cgggccggga atgcccatga
     9001 gcaacgatcc aatcccgctg ctgcgcccac cctccccaac tcgccaaccg ccgatgacga
     9061 cttcctgggt gttccagtgt ttgtccttga tccacgccga cgagcgccgc ccgggttgat
     9121 agccggagtc gcggcgcttg gcaatgactc cctcccaacc gcgcttgtgc gagtaggcca
     9181 gcgcctcggc gccgtcaccg ggcaacaact ccggaacgat cagattgccc gcactgccca
     9241 gggtttccag tagcttgcgc cggtcgcggt atctggcccg cagtagcgag cgaccgtcca
     9301 gaaagagcaa gtcgaacgcc cagaactcga tgcgggtggc gcgcacccgg ttctgcatct
     9361 cgttgaagct gggcactccg gaggagtcga gggccaccaa ttccccgtcg agcaccacgt
     9421 ggtgatcagc gagatcggcg gccaatgaac gtaattgggg gtattccgcg gtgacgtcac
     9481 gtccgctgcg ggagcgcagt cgcagcgcgc catggtcggc ttcgaccagc agccggtagc
     9541 cgtcccactt gccttcgaac gcccactgac ccgactcgag acctgccacc gaaccgtggg
     9601 aggtgagcat gggcgcgagg gtgtcgaaat cgaaggccgt ctggtccttc atgcgatgcg
     9661 ccagccattg gtccccgttg gtctgaatca gcgcgtaccg ccccgagatt cgactgccgt
     9721 gcaggttgac aatcacctcc cccttttccg aaccgtttct cgagtcgtcg cggaacttct
     9781 cggtgtcata ggtgccggaa tcccagatga tcaccttgcc ggcgccgtac tcccctttcg
     9841 ggatgttgcc ctcgaacgtc gcgtattcca gcggatgatc ttcggtgtgc acggccaggt
     9901 ggttcaccga cgtggtttcc ggcaggtttt tcggcaccgc ccatgacacc agtaccccat
     9961 cacgctccag cctgaagtcg taatgcagac ggcgcgcgtg atgctcttgg atcacgaagg
    10021 tattaccctg cccgacaacg ggtctggctc ggggcaccgg ctccggcgtt ttcgacgcgt
    10081 tgcgcatgct gcgatatttg agcagccggt cgtcgccggg gaggtccgcg tccagcgccg
    10141 caatcagatc gccatcgcgg gtgaggcggg caagcacctc gtcgtaggtc agctggcgca
    10201 gccccgggtc gtcgagctcc tcccagctgc gtggtgcggc gacggtcggt tgttcgcgcc
    10261 cacgcagcga atacggcgcg atggtggtct tcgaaccgct gttttggctc cagtccagga
    10321 acactttccc ggcccgcaga ctcttggtca tggtcgcggt gaccaacgcc ggcatcaccg
    10381 tttccagttg ctgcgcgacc cgtttggcca acaccgtggc gcccttgctg ctcaccggct
    10441 cgtcgagcgg ggtgtagagg tgcaggccct tgctaccgct ggtgagcggg agggtactca
    10501 ggccgatgtc ggcgagcaga tcgcgcaccg cccgcgccac ctgcgcgagt tgggccatcg
    10561 tcacgccctc gcccgggtcc aggtcgaaca ccaatcgggt ggccggcccg ggcgtgagga
    10621 tttcgccttt cgacgtccac tcggcaacga accgccactg cgggacgtgc acctcgaggg
    10681 cggcctgttg cgcgatccag gccagcccgg tggcgctgtc gatgatcgga taggtcgtcg
    10741 ttcccgaccg gtgcaccacg ctggcacgcg gcagccagtc cggcgccgac gacgccagct
    10801 gcttttcgaa aaaggacgcc tgatcgacgc cgttgggcca gcgtttgcga gtcgccgggc
    10861 gcccggcgat atgcgggatc atcacctcgg cgatgccggt gtagtaaccg aagacgtcag
    10921 acttggtggt tccggtagcc gggtacagca ccttgtccgc gttggtcagt gtcacctgcg
    10981 gaccattcgc ctgtccagga ggccaggcct gcgccgaacc catatggcca acgtaacctg
    11041 tcgccatggc acgtgggctg gtgatcggtg aagccctgat cgacatcgtc gatgggccgg
    11101 acccggccga gtatgtcggg ggcagccccc tcaacgtcgc ggtcggactg gccagattgg
    11161 gccgcgacgt cgatctgctg acccacatcg gccgcgacgc ccgcggacga cggatcgccg
    11221 agtacattga atcctcaggt gtacaacttg tttcgggaag ccagacggct gatcggacgc
    11281 ccaccgcgac ggccaggatc ggccccgacg gctcggccac ctacgcgttc gacctggaat
    11341 ggcagatacc cgacacaccc cccgtggcgc cgccgcttct cgtgcacacc ggttccatcg
    11401 ccgccgcgcg ggaaccagga tgcttggcgg tcgcggcgct gctcgacgcc tatcgggccg
    11461 cggccacggt gagcttcgat cccaatgtgc gtccgtcgct gagcgccgac ccggacctga
    11521 ctcgggaacg catccaacgc ctcgtcgagc gcagcgacat catcaaggcc agtgccgaag
    11581 acttgcactg gatcgacccc acccagcctc cggagcagac cgcgcgggcc tggctggcct
    11641 gcggcccggc gattgtcgcg ctgacgttgg gcgatcaggg cgcagtggcg ttctgcgcgg
    11701 ccggcccggc aagtgtgccc gcccagccgg ttcgcgtggt cgataccgtc ggcgccggag
    11761 acgcgttcat ggccggtctg ctcgacacgc tgtgggagca gggattgtta ggtgccgacc
    11821 ggcgcaccga gttgcgcaag atcggcgtgt cggcgctgac ctctgcactc gaagtcgcgg
    11881 ccctgacctc ggcgttgacc gttgcccgtg ccggcgccga tctgccctat cgggccgacc
    11941 tgcggcagtc gcgatgacca ccgcacgccc gcaatcgggc ccggcgaggg tgcacgaaat
    12001 gccgcgtgat gcggcgtgtc gccgtgccga cacgcacgct cgcgagctga ccggggctgg
    12061 ggcaggcagc aggactccgg tgccgcggcg cactgttaac cgacccgggt tctgggcata
    12121 ctgactccat gcgctccatc tggaagggtt caatcgcttt cgggctggtg aatgtgccgg
    12181 tcaaggtgta cagcgccact caggaccacg acatcaagtt ccatcaggtg cacgccaagg
    12241 acaacggacg catccggtac aagcgggtat gcgaggtctg tggcgaggtg gtcgaatact
    12301 ccgacctggc ccgcgccttc gaatccgacg atggccagat ggtgatcatc aacgacgacg
    12361 acctcgccac gttgcccgaa gatcgcagcc gcgaaatcga ggtgctggaa ttcgtcccag
    12421 ccgaagaagt cgatccgatg atgattgacc gcagctactt cctggagccc gactcgaagt
    12481 cctctaaatc gtatgtgctg ctggccaaga cgctcgccga gacggagcgg atggcgatcg
    12541 tgcatttcac gctgcgcagc aagacgcggc tggcggcgtt gcgggtcaag gacttcggca
    12601 agcgcgaggt catggtggtg cataccctgt tgtggccaga cgagatccgc gacccggatt
    12661 tcccggtgct ggacaaggag gtggagatca aacccgcgga gctcaagatg gccggccaag
    12721 tggtggagtc gatggcagac gacttcaacc cggaccgcta ccgcgacacc tatcaggagc
    12781 aactgcagga gctgatcgac gccaaactcg aaggcgggga agcatttacc gccgaagaac
    12841 aacccgcaga gctggacgag accgaagatg tctccgacct gctggccaag ctggaggcga
    12901 gcgtgaaagc gcgttcgggc gacgggaagt cagcggcgaa gaagagcacc gccgaaaagg
    12961 cgccggccaa gaaggcccgg gccaaacaag ctgccaagtc ctgaccagcg cccgacttgt
    13021 tcacatatgc tcgtcgtgcc gccgagagga gtttttgcgt gaagtccaac cgatctggcg
    13081 tcgtgctcag cctgctgctc gccagcacat tggcgttgtc ggcatgcgga ggccattcca
    13141 gcaatacctc gtccacctga cgtcgggggg gggggaaagc cac
//
