LOCUS       sequence-321969-       10314 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TU for the expression of chimeric construct comprising the 
            N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to
            the VP16 activation domain and a nuclear localization sequence. Part
            of a red light-controlled synthetic genetic switch.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10314)
  TITLE     Orzaez lab plasmids
REFERENCE   2  (bases 1 to 10314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEGB3_alpha2 35:PhyB:VP16  (GB2745)
FEATURES             Location/Qualifiers
     source          1..10314
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        637..981
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     925..945
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             3209..3442
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3443..3463
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      3490..3742
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3777..3795)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    3979..4003
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5304..5933
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6362..7435
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7501..7695
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7931..7953)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8039..8179
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8094..8113
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8194..8211)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8365..8953)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8445..8464)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9041..9856)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9041..9856)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9764..9783
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    10289..10313
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 taacaagctt cgtctcagtc aggagactag agccaagctg atctcctttg ccccggagat
       61 caccatggac gactttctct atctctacga tctaggaaga aagttcgacg gagaaggtga
      121 cgataccatg ttcaccaccg ataatgagaa gattagcctc ttcaatttca gaaagaatgc
      181 tgacccacag atggttagag aggcctacgc ggcaggtctg atcaagacga tctacccgag
      241 taataatctc caggagatca aataccttcc caagaaggtt aaagatgcag tcaaaagatt
      301 caggactaac tgcatcaaga acacagagaa agatatattt ctcaagatca gaagtactat
      361 tccagtatgg acgattcaag gcttgcttca taaaccaagg caagtaatag agattggagt
      421 ctctaagaaa gtagttccta ctgaatcaaa ggccatggag tcaaaaattc agatcgagga
      481 tctaacagaa ctcgccgtga agactggcga acagttcata cagagtcttt tacgactcaa
      541 tgacaagaag aaaatcttcg tcaacatggt ggagcacgac actctcgtct actccaagaa
      601 tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac aaagggtaat
      661 atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca aaaggacagt
      721 agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca
      781 agatgcccct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
      841 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata tctccactga
      901 cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag
      961 ttcatttcat ttggagagga ctccggtatt tttacaacaa ttaccacaac aaaacaaaca
     1021 acaaacaaca ttacaattta ctattctagt cgaaatgaca acaatggttt ccggagtcgg
     1081 gggtagtggc ggtggccgtg gcggtggccg tggcggagaa gaagaaccgt cgtcaagtca
     1141 cactcctaat aaccgaagag gaggagaaca agctcaatcg tcgggaacga aatctctcag
     1201 accaagaagc aacactgaat caatgagcaa agcaattcaa cagtacaccg tcgacgcaag
     1261 actccacgcc gttttcgaac aatccggcga atcagggaaa tcattcgact actcacaatc
     1321 actcaaaacg acgacgtacg gttcctctgt acctgagcaa cagatcacag cttatctctc
     1381 tcgaatccag cgaggtggtt acattcagcc tttcggatgt atgatcgccg tcgatgaatc
     1441 cagtttccgg atcatcggtt acagtgaaaa cgccagagaa atgttaggga ttatgcctca
     1501 atctgttcct actcttgaga aacctgagat tctagctatg ggaactgatg tgagatcttt
     1561 gttcacttct tcgagctcga ttctactcga gcgtgctttc gttgctcgag agattacctt
     1621 gttaaatccg gtttggatcc attccaagaa tactggtaaa ccgttttacg ccattcttca
     1681 taggattgat gttggtgttg ttattgattt agagccagct agaactgaag atcctgcgct
     1741 ttctattgct ggtgctgttc aatcgcagaa actcgcggtt cgtgcgattt ctcagttaca
     1801 ggctcttcct ggtggagata ttaagctttt gtgtgacact gtcgtggaaa gtgtgaggga
     1861 cttgactggt tatgatcgtg ttatggttta taagtttcat gaagatgagc atggagaagt
     1921 tgtagctgag agtaaacgag atgatttaga gccttatatt ggactgcatt atcctgctac
     1981 tgatattcct caagcgtcaa ggttcttgtt taagcagaac cgtgtccgaa tgatagtaga
     2041 ttgcaatgcc acacctgttc ttgtggtcca ggacgatagg ctaactcagt ctatgtgctt
     2101 ggttggttct actcttaggg ctcctcatgg ttgtcactct cagtatatgg ctaacatggg
     2161 atctattgcg tctttagcaa tggcggttat aatcaatgga aatgaagatg atgggagcaa
     2221 tgtagctagt ggaagaagct cgatgaggct ttggggtttg gttgtttgcc atcacacttc
     2281 ttctcgctgc ataccgtttc cgctaaggta tgcttgtgag tttttgatgc aggctttcgg
     2341 tttacagtta aacatggaat tgcagttagc tttgcaaatg tcagagaaac gcgttttgag
     2401 aacgcagaca ctgttatgtg atatgcttct gcgtgactcg cctgctggaa ttgttacaca
     2461 gagtcccagt atcatggact tagtgaaatg tgacggtgca gcatttcttt accacgggaa
     2521 gtattacccg ttgggtgttg ctcctagtga agttcagata aaagatgttg tggagtggtt
     2581 gcttgcgaat catgcggatt caaccggatt aagcactgat agtttaggcg atgcggggta
     2641 tcccggtgca gctgcgttag gggatgctgt gtgcggtatg gcagttgcat atatcacaaa
     2701 aagagacttt cttttttggt ttcgatctca cactgcgaaa gaaatcaaat ggggaggcgc
     2761 taagcatcat ccggaggata aagatgatgg gcaacgaatg catcctcgtt cgtcctttca
     2821 ggcttttctt gaagttgtta agagccggag tcagccatgg gaaactgcgg aaatggatgc
     2881 gattcactcg ctccagctta ttctgagaga ctcttttaaa gaatctgagg cggctatgaa
     2941 ctctaaagtt gtggatggtg tggttcagcc atgtagggat atggcggggg aacaggggat
     3001 tgatgagtta ggtgaattcg atagtgctgg tagtgctggt agtgctggtt cgggttccgc
     3061 gtacagccgc gcgcgtacga aaaacaatta cgggtctacc atcgagggcc tgctcgatct
     3121 cccggacgac gacgcccccg aagaggcggg gctggcggct ccgcgcctgt cctttctccc
     3181 cgcgggacac acgcgcagac tgtcgacggc ccccccgacc gatgtcagcc tgggggacga
     3241 gctccactta gacggcgagg acgtggcgat ggcgcatgcc gacgcgctag acgatttcga
     3301 tctggacatg ttgggggacg gggattcccc gggtccggga tttacccccc acgactccgc
     3361 cccctacggc gctctggata tggccgactt cgagtttgag cagatgttta ccgatgccct
     3421 tggaattgac gagtacggtg ggccaaagaa gaagaggaag gtgtaggctt ggaatggatc
     3481 ttcgatcccg atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
     3541 ggtcttgcga cgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
     3601 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     3661 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
     3721 gtgtcatcta tgttactaga tcgggaattg ccaagctaat tcttgaagac gaaagggcct
     3781 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     3841 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     3901 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat gggaccgact
     3961 cgcgcttgag acgaagcttg acaggatata ttggcgggta aacctaagag aaaagagcgt
     4021 ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt
     4081 atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg
     4141 ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcatctgaaa acgacatgtc
     4201 gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt
     4261 cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat
     4321 gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga
     4381 cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa
     4441 gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc
     4501 tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact
     4561 ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg
     4621 ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga
     4681 gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg
     4741 aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga
     4801 gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg
     4861 ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg
     4921 gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa
     4981 tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc
     5041 attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg
     5101 cacctctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg
     5161 gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt
     5221 gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat
     5281 aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt
     5341 caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg
     5401 ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag
     5461 atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca
     5521 tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt
     5581 ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat
     5641 gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc
     5701 tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg
     5761 ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga
     5821 gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg
     5881 ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg
     5941 aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg
     6001 cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa
     6061 ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa
     6121 gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg
     6181 aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac
     6241 caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa
     6301 gcggctgggt tgcctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg
     6361 cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct
     6421 ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg
     6481 ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc
     6541 ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt
     6601 cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt
     6661 tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga
     6721 cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct
     6781 ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg
     6841 agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg
     6901 agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac
     6961 gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga
     7021 gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta
     7081 catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga
     7141 cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta
     7201 ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta
     7261 cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat
     7321 cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat
     7381 cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac
     7441 ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gactctttcc
     7501 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
     7561 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
     7621 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
     7681 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
     7741 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
     7801 ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc
     7861 gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg
     7921 acggtgaaaa cctctgacac atgcagctcc cggtgacggt cacagcttgt ctgtaagcgg
     7981 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
     8041 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
     8101 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     8161 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     8221 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     8281 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     8341 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8401 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8461 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8521 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     8581 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8641 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8701 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8761 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     8821 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8881 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8941 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     9001 aaactcacgt taagggattt tggtcatgca ttctaggtga ttagaaaaac tcatcgagca
     9061 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
     9121 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
     9181 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
     9241 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
     9301 aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
     9361 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agtcgaaata
     9421 cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
     9481 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
     9541 ctgttttccc tgggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
     9601 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg
     9661 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
     9721 tcccatacaa tcggtagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
     9781 acccatataa atcagcatcc atgttggaat ttaatcgcgg ccttgagcaa gacgtttccc
     9841 gttgaatatg gctcataaca gaacttatta tttccttcct cttttctaca gtatttaaag
     9901 ataccccaag aagctaatta taacaagacg aactccaatt cactgttcct tgcattctaa
     9961 aaccttaaat accagaaaac agctttttca aagttgtttt caaagttggc gtataacata
    10021 gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt
    10081 tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg
    10141 ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc
    10201 cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct
    10261 gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta aaca
//
