LOCUS       sequence-321990-       11244 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TU for the expression of LbCas12a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11244)
  TITLE     Orzaez lab plasmids
REFERENCE   2  (bases 1 to 11244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDGB3 alpha2 AtUBQ10:LbCas12a:Tnos (GB2479)
FEATURES             Location/Qualifiers
     source          1..11244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    181..205
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             865..4548
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             4549..4596
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      4626..4878
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4913..4931)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    5115..5139
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6440..7069
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7498..8571
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8637..8831
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9067..9089)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9175..9315
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9230..9249
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9330..9347)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9501..10089)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9581..9600)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10177..10992)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(10177..10992)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10900..10919
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat
       61 gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct
      121 gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg
      181 tggcaggata tattgtggtg taaacataac aagcttcgtc tcagtcagga gtcgacgagt
      241 cagtaataaa cggcgtcaaa gtggttgcag ccggcacaca cgagtcgtgt ttatcaactc
      301 aaagcacaaa tacttttcct caacctaaaa ataaggcaat tagccaaaaa caactttgcg
      361 tgtaaacaac gctcaataca cgtgtcattt tattattagc tattgcttca ccgccttagc
      421 tttctcgtga cctagtcgtc ctcgtctttt cttcttcttc tataaaacaa tacccaaaga
      481 gctcttcttc ttcacaattc agatttcaat ttctcaaaat cttaaaaact ttctctcaat
      541 tctctctacc gtgatcaagg taaatttctg tgttccttat tctctcaaaa tcttcgattt
      601 tgttttcgtt cgatcccaat ttcgtatatg ttctttggtt tagattctgt taatcttaga
      661 tcgaagacga ttttctgggt ttgatcgtta gatatcatct taattctcga ttagggtttc
      721 atagatatca tccgatttgt tcaaataatt tgagttttgt cgaataatta ctcttcgatt
      781 tgtgatttct atctagatct ggtgttagtt tctagtttgt gcgatcgaat ttgtcgatta
      841 atctgagttt ttctgattaa cagaatgagc aagctggaga agtttacaaa ctgctactcc
      901 ctgtctaaga ccctgaggtt caaggccatc cctgtgggca agacccagga gaacatcgac
      961 aataagcggc tgctggtgga ggacgagaag agagccgagg attataaggg cgtgaagaag
     1021 ctgctggatc gctactatct gtcttttatc aacgacgtgc tgcacagcat caagctgaag
     1081 aatctgaaca attacatcag cctgttccgg aagaaaacca gaaccgagaa ggagaataag
     1141 gagctggaga acctggagat caatctgcgg aaggagatcg ccaaggcctt caagggcaac
     1201 gagggctaca agtccctgtt taagaaggat atcatcgaga caatcctgcc agagttcctg
     1261 gacgataagg acgagatcgc cctggtgaac agcttcaatg gctttaccac agccttcacc
     1321 ggcttctttg ataacagaga gaatatgttt tccgaggagg ccaagagcac atccatcgcc
     1381 ttcaggtgta tcaacgagaa tctgacccgc tacatctcta atatggacat cttcgagaag
     1441 gtggacgcca tctttgataa gcacgaggtg caggagatca aggagaagat cctgaacagc
     1501 gactatgatg tggaggattt ctttgagggc gagttcttta actttgtgct gacacaggag
     1561 ggcatcgacg tgtataacgc catcatcggc ggcttcgtga ccgagagcgg cgagaagatc
     1621 aagggcctga acgagtacat caacctgtat aatcagaaaa ccaagcagaa gctgcctaag
     1681 tttaagccac tgtataagca ggtgctgagc gatcgggagt ctctgagctt ctacggcgag
     1741 ggctatacat ccgatgagga ggtgctggag gtgtttagaa acaccctgaa caagaacagc
     1801 gagatcttca gctccatcaa gaagctggag aagctgttca agaattttga cgagtactct
     1861 agcgccggca tctttgtgaa gaacggcccc gccatcagca caatctccaa ggatatcttc
     1921 ggcgagtgga acgtgatccg ggacaagtgg aatgccgagt atgacgatat ccacctgaag
     1981 aagaaggccg tggtgaccga gaagtacgag gacgatcgga gaaagtcctt caagaagatc
     2041 ggctcctttt ctctggagca gctgcaggag tacgccgacg ccgatctgtc tgtggtggag
     2101 aagctgaagg agatcatcat ccagaaggtg gatgagatct acaaggtgta tggctcctct
     2161 gagaagctgt tcgacgccga ttttgtgctg gagaagagcc tgaagaagaa cgacgccgtg
     2221 gtggccatca tgaaggacct gctggattct gtgaagagct tcgagaatta catcaaggcc
     2281 ttctttggcg agggcaagga gacaaacagg gacgagtcct tctatggcga ttttgtgctg
     2341 gcctacgaca tcctgctgaa ggtggaccac atctacgatg ccatccgcaa ttatgtgacc
     2401 cagaagccct actctaagga taagttcaag ctgtattttc agaaccctca gttcatgggc
     2461 ggctgggaca aggataagga gacagactat cgggccacca tcctgagata cggctccaag
     2521 tactatctgg ccatcatgga taagaagtac gccaagtgcc tgcagaagat cgacaaggac
     2581 gatgtgaacg gcaattacga gaagatcaac tataagctgc tgcccggccc taataagatg
     2641 ctgccaaagg tgttcttttc taagaagtgg atggcctact ataaccccag cgaggacatc
     2701 cagaagatct acaagaatgg cacattcaag aagggcgata tgtttaacct gaatgactgt
     2761 cacaagctga tcgacttctt taaggatagc atctcccggt atccaaagtg gtccaatgcc
     2821 tacgatttca acttttctga gacagagaag tataaggaca tcgccggctt ttacagagag
     2881 gtggaggagc agggctataa ggtgagcttc gagtctgcca gcaagaagga ggtggataag
     2941 ctggtggagg agggcaagct gtatatgttc cagatctata acaaggactt ttccgataag
     3001 tctcacggca cacccaatct gcacaccatg tacttcaagc tgctgtttga cgagaacaat
     3061 cacggacaga tcaggctgag cggaggagca gagctgttca tgaggcgcgc ctccctgaag
     3121 aaggaggagc tggtggtgca cccagccaac tcccctatcg ccaacaagaa tccagataat
     3181 cccaagaaaa ccacaaccct gtcctacgac gtgtataagg ataagaggtt ttctgaggac
     3241 cagtacgagc tgcacatccc aatcgccatc aataagtgcc ccaagaacat cttcaagatc
     3301 aatacagagg tgcgcgtgct gctgaagcac gacgataacc cctatgtgat cggcatcgat
     3361 aggggcgagc gcaatctgct gtatatcgtg gtggtggacg gcaagggcaa catcgtggag
     3421 cagtattccc tgaacgagat catcaacaac ttcaacggca tcaggatcaa gacagattac
     3481 cactctctgc tggacaagaa ggagaaggag aggttcgagg cccgccagaa ctggacctcc
     3541 atcgagaata tcaaggagct gaaggccggc tatatctctc aggtggtgca caagatctgc
     3601 gagctggtgg agaagtacga tgccgtgatc gccctggagg acctgaactc tggctttaag
     3661 aatagccgcg tgaaggtgga gaagcaggtg tatcagaagt tcgagaagat gctgatcgat
     3721 aagctgaact acatggtgga caagaagtct aatccttgtg caacaggcgg cgccctgaag
     3781 ggctatcaga tcaccaataa gttcgagagc tttaagtcca tgtctaccca gaacggcttc
     3841 atcttttaca tccctgcctg gctgacatcc aagatcgatc catctaccgg ctttgtgaac
     3901 ctgctgaaaa ccaagtatac cagcatcgcc gattccaaga agttcatcag ctcctttgac
     3961 aggatcatgt acgtgcccga ggaggatctg ttcgagtttg ccctggacta taagaacttc
     4021 tctcgcacag acgccgatta catcaagaag tggaagctgt actcctacgg caaccggatc
     4081 agaatcttcc ggaatcctaa gaagaacaac gtgttcgact gggaggaggt gtgcctgacc
     4141 agcgcctata aggagctgtt caacaagtac ggcatcaatt atcagcaggg cgatatcaga
     4201 gccctgctgt gcgagcagtc cgacaaggcc ttctactcta gctttatggc cctgatgagc
     4261 ctgatgctgc agatgcggaa cagcatcaca ggccgcaccg acgtggattt tctgatcagc
     4321 cctgtgaaga actccgacgg catcttctac gatagccgga actatgaggc ccaggagaat
     4381 gccatcctgc caaagaacgc cgacgccaat ggcgcctata acatcgccag aaaggtgctg
     4441 tgggccatcg gccagttcaa gaaggccgag gacgagaagc tggataaggt gaagatcgcc
     4501 atctctaaca aggagtggct ggagtacgcc cagaccagcg tgaagcacaa aaggccggcg
     4561 gccacgaaaa aggccggcca ggcaaaaaag aaaaagtagt gagcttggaa tggatcttcg
     4621 atcccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
     4681 ttgcgacgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
     4741 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
     4801 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
     4861 catctatgtt actagatcgg gaattgccaa gctaattctt gaagacgaaa gggcctcgtg
     4921 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     4981 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     5041 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatggga ccgactcgcg
     5101 cttgagacga agcttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat
     5161 tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt
     5221 gcatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc cctccgctgc
     5281 tatagtgcag tcggcttctg acgttcagtg cagccgtcat ctgaaaacga catgtcgcac
     5341 aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg
     5401 tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac
     5461 aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg
     5521 accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc
     5581 accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc
     5641 gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac
     5701 attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc
     5761 gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc
     5821 gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc
     5881 gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg
     5941 atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg
     6001 accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc
     6061 ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa
     6121 cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta
     6181 ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacc
     6241 tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct
     6301 ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat
     6361 ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac
     6421 aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg
     6481 caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct
     6541 gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca
     6601 accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg
     6661 ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc
     6721 gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc
     6781 caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca
     6841 agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga
     6901 ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta
     6961 cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc
     7021 ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt
     7081 aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc
     7141 agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt
     7201 cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc
     7261 attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata
     7321 aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg
     7381 caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg
     7441 ctgggttgcc tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg
     7501 agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg
     7561 gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc
     7621 ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca
     7681 gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt
     7741 ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc
     7801 cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg
     7861 cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta
     7921 ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac
     7981 aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc
     8041 gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac
     8101 gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt
     8161 gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc
     8221 gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg
     8281 ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc
     8341 ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa
     8401 cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg
     8461 tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta
     8521 gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag
     8581 cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggact ctttcctgtg
     8641 gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg
     8701 aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa
     8761 aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg
     8821 gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt
     8881 cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc
     8941 tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg
     9001 ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg
     9061 tgaaaacctc tgacacatgc agctcccggt gacggtcaca gcttgtctgt aagcggatgc
     9121 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     9181 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
     9241 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     9301 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     9361 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     9421 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     9481 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     9541 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     9601 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     9661 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     9721 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     9781 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     9841 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     9901 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     9961 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    10021 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    10081 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    10141 tcacgttaag ggattttggt catgcattct aggtgattag aaaaactcat cgagcatcaa
    10201 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
    10261 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
    10321 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
    10381 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
    10441 tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
    10501 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagtc gaaatacgcg
    10561 atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc
    10621 cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt
    10681 tttccctggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt
    10741 gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac
    10801 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc
    10861 atacaatcgg tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc
    10921 atataaatca gcatccatgt tggaatttaa tcgcggcctt gagcaagacg tttcccgttg
    10981 aatatggctc ataacagaac ttattatttc cttcctcttt tctacagtat ttaaagatac
    11041 cccaagaagc taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc
    11101 ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat
    11161 cgacggagcc gattttgaaa ccgcggtgat cacaggcagc aacgctctgt catcgttaca
    11221 atcaacatgc taccctccgc gaga
//
