LOCUS       sequence-321993-       15565 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for the constitutive expression of the nptII, Cas9 and DsRed 
            genes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15565)
  AUTHORS   Vazquez-Vilar M, Garcia-Carpintero V, Selma S, Bernabe-Orts JM, 
            Sanchez-Vicente J, Salazar-Sarasua B, Ressa A, de Paola C, Ajenjo M,
            Quintela JC, Fernandez-Del-Carmen A, Granell A, Orzaez D
  TITLE     The GB4.0 Platform, an All-In-One Tool for CRISPR/Cas-Based 
            Multiplex Genome Engineering in Plants.
  JOURNAL   Front Plant Sci. 2021 Jul 1;12:689937. doi: 
            10.3389/fpls.2021.689937. eCollection 2021.
  PUBMED    34276739
REFERENCE   2  (bases 1 to 15565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2021-07-1"; volume: "12:689937. doi"; pages: " 
            10.3389/fpls.2021.689937. eCollection 2021"
COMMENT     Sequence Label: pDGB3 alpha1 Tnos:nptII:Pnos - P35S:Cas9:Tnos ...
FEATURES             Location/Qualifiers
     source          1..15565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(28..280)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(320..1114)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(431..450)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1041..1060
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1148..1327)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2029..2373
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             2447..6550
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2447..6550
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2447..6550
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6563..6583
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6610..6862
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6897..6915)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7706..8050
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             8124..8801
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(8304..8324)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     8716..8739
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    9214..9238
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10539..11168
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11597..12670
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      12736..12930
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(13166..13188)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13274..13414
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13329..13348
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13429..13446)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13600..14188)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13680..13699)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(14276..15091)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(14276..15091)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     14999..15018
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    15524..15548
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagagcgat cagcttgcat gccggtcgat ctagtaacat agatgacacc gcgcgcgata
       61 atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg tataattgcg
      121 ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg ttaattatta
      181 catgcttaac gtaattcaac agaaattata tgataatcat ggcaagaccg gcaacaggat
      241 tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc tgcttgactc tagctagagt
      301 ccgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag gctatgcgct
      361 gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa
      421 gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca
      481 gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc
      541 aggcgtcgcc gtgggtcacg acgagatcct cgccgtcggg catccgcgcc ttgagcctgg
      601 cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa
      661 gaccggcttc catccgagta cgtgctcgct cgatgcggtg tttcgcttgg tggtcgaatg
      721 ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt
      781 tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca
      841 gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg
      901 tggccagcca cgatagccgc gctgcctcgt cttggagttc attcagggca ccggacaggt
      961 cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag
     1021 agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg
     1081 gagaacctgc gtgcaatcca tcttgttcaa tcatgcctcg atcgagttga gagtgaatat
     1141 gagactctaa ttggataccg aggggaattt atggaacgtc agtggagcat ttttgacaag
     1201 aaatatttgc tagctgatag tgaccttagg cgacttttga acgcgcaata atggtttctg
     1261 acgtatgtgc ttagctcatt aaactccaga aacccgcggc tcagtggctc cttcaacgtt
     1321 gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg ggggtcatac
     1381 cgtgactccc ttaattctca tgtatctccg tcaggagact agagccaagc tgatctcctt
     1441 tgccccggag atcaccatgg acgactttct ctatctctac gatctaggaa gaaagttcga
     1501 cggagaaggt gacgatacca tgttcaccac cgataatgag aagattagcc tcttcaattt
     1561 cagaaagaat gctgacccac agatggttag agaggcctac gcggcaggtc tgatcaagac
     1621 gatctacccg agtaataatc tccaggagat caaatacctt cccaagaagg ttaaagatgc
     1681 agtcaaaaga ttcaggacta actgcatcaa gaacacagag aaagatatat ttctcaagat
     1741 cagaagtact attccagtat ggacgattca aggcttgctt cataaaccaa ggcaagtaat
     1801 agagattgga gtctctaaga aagtagttcc tactgaatca aaggccatgg agtcaaaaat
     1861 tcagatcgag gatctaacag aactcgccgt gaagactggc gaacagttca tacagagtct
     1921 tttacgactc aatgacaaga agaaaatctt cgtcaacatg gtggagcacg acactctcgt
     1981 ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg agacttttca
     2041 acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
     2101 caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg ataaaggaaa
     2161 ggctatcgtt caagatgccc ctgccgacag tggtcccaaa gatggacccc cacccacgag
     2221 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
     2281 tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
     2341 tatataagga agttcatttc atttggagag gactccggta tttttacaac aattaccaca
     2401 acaaaacaaa caacaaacaa cattacaatt tactattcta gtcgaaatgg acaagaagta
     2461 ctccattggg ctcgatatcg gcacaaacag cgtcggctgg gccgtcatta cggacgagta
     2521 caaggtgccg agcaaaaaat tcaaagttct gggcaatacc gatcgccaca gcataaagaa
     2581 gaacctcatt ggcgccctcc tgttcgactc cggggaaacg gccgaagcca cgcggctcaa
     2641 aagaacagca cggcgcagat atacccgcag aaagaatcgg atctgctacc tgcaggagat
     2701 ctttagtaat gagatggcta aggtggatga ctctttcttc cataggctgg aggagtcctt
     2761 tttggtggag gaggataaaa agcacgagcg ccacccaatc tttggcaata tcgtggacga
     2821 ggtggcgtac catgaaaagt acccaaccat atatcatctg aggaagaagc ttgtagacag
     2881 tactgataag gctgacttgc ggttgatcta tctcgcgctg gcgcatatga tcaaatttcg
     2941 gggacacttc ctcatcgagg gggacctgaa cccagacaac agcgatgtcg acaaactctt
     3001 tatccaactg gttcagactt acaatcagct tttcgaagag aacccgatca acgcatccgg
     3061 agttgacgcc aaagcaatcc tgagcgctag gctgtccaaa tcccggcggc tcgaaaacct
     3121 catcgcacag ctccctgggg agaagaagaa cggcctgttt ggtaatctta tcgccctgtc
     3181 actcgggctg acccccaact ttaaatctaa cttcgacctg gccgaagatg ccaagcttca
     3241 actgagcaaa gacacctacg atgatgatct cgacaatctg ctggcccaga tcggcgacca
     3301 gtacgcagac ctttttttgg cggcaaagaa cctgtcagac gccattctgc tgagtgatat
     3361 tctgcgagtg aacacggaga tcaccaaagc tccgctgagc gctagtatga tcaagcgcta
     3421 tgatgagcac caccaagact tgactttgct gaaggccctt gtcagacagc aactgcctga
     3481 gaagtacaag gaaattttct tcgatcagtc taaaaatggc tacgccggat acattgacgg
     3541 cggagcaagc caggaggaat tttacaaatt tattaagccc atcttggaaa aaatggacgg
     3601 caccgaggag ctgctggtaa agcttaacag agaagatctg ttgcgcaaac agcgcacttt
     3661 cgacaatgga agcatccccc accagattca cctgggcgaa ctgcacgcta tcctcaggcg
     3721 gcaagaggat ttctacccct ttttgaaaga taacagggaa aagattgaga aaatcctcac
     3781 atttcggata ccctactatg taggccccct cgcccgggga aattccagat tcgcgtggat
     3841 gactcgcaaa tcagaagaga ctatcactcc ctggaacttc gaggaagtcg tggataaggg
     3901 ggcctctgcc cagtccttca tcgaaaggat gactaacttt gataaaaatc tgcctaacga
     3961 aaaggtgctt cctaaacact ctctgctgta cgagtacttc acagtttata acgagctcac
     4021 caaggtcaaa tacgtcacag aagggatgag aaagccagca ttcctgtctg gagagcagaa
     4081 gaaagctatc gtggacctcc tcttcaagac gaaccggaaa gttaccgtga aacagctcaa
     4141 agaagattat ttcaaaaaga ttgaatgttt cgactctgtt gaaatcagcg gagtggagga
     4201 tcgcttcaac gcatccctgg gaacgtatca cgatctcctg aaaatcatta aagacaagga
     4261 cttcctggac aatgaggaga acgaggacat tcttgaggac attgtcctca cccttacgtt
     4321 gtttgaagat agggagatga ttgaagaacg cttgaaaact tacgctcatc tcttcgacga
     4381 caaagtcatg aaacagctca agaggcgccg atatacagga tgggggcggc tgtcaagaaa
     4441 actgatcaat gggatccgag acaagcagag tggaaagaca atcctggatt ttcttaagtc
     4501 cgatggattt gccaaccgga acttcatgca gttgatccat gatgactctc tcacctttaa
     4561 ggaggacatc cagaaagcac aagtttctgg ccagggggac agtctccacg agcacatcgc
     4621 taatcttgca ggtagcccag ctatcaaaaa gggaatactg cagaccgtta aggtcgtgga
     4681 tgaactcgtc aaagtaatgg gaaggcataa gcccgagaat atcgttatcg agatggcccg
     4741 agagaaccaa actacccaga agggacagaa gaacagtagg gaaaggatga agaggattga
     4801 agagggtata aaagaactgg ggtcccaaat ccttaaggaa cacccagttg aaaacaccca
     4861 gcttcagaat gagaagctct acctgtacta cctgcagaac ggcagggaca tgtacgtgga
     4921 tcaggaactg gacatcaatc ggctctccga ctacgacgtg gatcatatcg tgccccagtc
     4981 ttttctcaaa gatgattcta ttgataataa agtgttgaca agatccgata aaaatagagg
     5041 gaagagtgat aacgtcccct cagaagaagt tgtcaagaaa atgaaaaatt attggcggca
     5101 gctgctgaac gccaaactga tcacacaacg gaagttcgat aatctgacta aggctgaacg
     5161 aggtggcctg tctgagttgg ataaagccgg cttcatcaaa aggcagcttg ttgagacacg
     5221 ccagatcacc aagcacgtgg cccaaattct cgattcacgc atgaacacca agtacgatga
     5281 aaatgacaaa ctgattcgag aggtgaaagt tattactctg aagtctaagc tggtttcaga
     5341 tttcagaaag gactttcagt tttataaggt gagagagatc aacaattacc accatgcgca
     5401 tgatgcctac ctgaatgcag tggtaggcac tgcacttatc aaaaaatatc ccaagcttga
     5461 atctgaattt gtttacggag actataaagt gtacgatgtt aggaaaatga tcgcaaagtc
     5521 tgagcaggaa ataggcaagg ccaccgctaa gtacttcttt tacagcaata ttatgaattt
     5581 tttcaagacc gagattacac tggccaatgg agagattcgg aagcgaccac ttatcgaaac
     5641 aaacggagaa acaggagaaa tcgtgtggga caagggtagg gatttcgcga cagtccggaa
     5701 ggtcctgtcc atgccgcagg tgaacatcgt taaaaagacc gaagtacaga ccggaggctt
     5761 ctccaaggaa agtatcctcc cgaaaaggaa cagcgacaag ctgatcgcac gcaaaaaaga
     5821 ttgggacccc aagaaatacg gcggattcga ttctcctaca gtcgcttaca gtgtactggt
     5881 tgtggccaaa gtggagaaag ggaagtctaa aaaactcaaa agcgtcaagg aactgctggg
     5941 catcacaatc atggagcgat caagcttcga aaaaaacccc atcgactttc tcgaggcgaa
     6001 aggatataaa gaggtcaaaa aagacctcat cattaagctt cccaagtact ctctctttga
     6061 gcttgaaaac ggccggaaac gaatgctcgc tagtgcgggc gagctgcaga aaggtaacga
     6121 gctggcactg ccctctaaat acgttaattt cttgtatctg gccagccact atgaaaagct
     6181 caaaggatct cccgaagata atgagcagaa gcagctgttc gtggaacaac acaaacacta
     6241 ccttgatgag atcatcgagc aaataagcga attctccaaa agagtgatcc tcgccgacgc
     6301 taacctcgat aaggtgcttt ctgcttacaa taagcacagg gataagccca tcagggagca
     6361 ggcagaaaac attatccact tgtttactct gaccaacttg ggcgcgcctg cagccttcaa
     6421 gtacttcgac accaccatag acagaaagcg gtacacctct acaaaggagg tcctggacgc
     6481 cacactgatt catcagtcaa ttacggggct ctatgaaaca agaatcgacc tctctcagct
     6541 cggtggagac agcagggctg accccaagaa gaagaggaag gtgtgagctt ggaatggatc
     6601 ttcgatcccg atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
     6661 ggtcttgcga cgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
     6721 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     6781 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
     6841 gtgtcatcta tgttactaga tcgggaattg ccaagctaat tcttgaagac gaaagggcct
     6901 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     6961 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     7021 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat gggaccgact
     7081 cgcgctgtca ggagactaga gccaagctga tctcctttgc cccggagatc accatggacg
     7141 actttctcta tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt
     7201 tcaccaccga taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga
     7261 tggttagaga ggcctacgcg gcaggtctga tcaagacgat ctacccgagt aataatctcc
     7321 aggagatcaa ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact
     7381 gcatcaagaa cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga
     7441 cgattcaagg cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag
     7501 tagttcctac tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac
     7561 tcgccgtgaa gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga
     7621 aaatcttcgt caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata
     7681 cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc
     7741 tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag
     7801 gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcccctg
     7861 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     7921 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
     7981 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     8041 tggagaggac tccggtattt ttacaacaat taccacaaca aaacaaacaa caaacaacat
     8101 tacaatttac tattctagtc gaaatggcct cctccgagaa cgtcatcacc gagttcatgc
     8161 gcttcaaggt gcgcatggag ggcaccgtga acggccacga gttcgagatc gagggcgagg
     8221 gcgagggccg cccctacgag ggccacaaca ccgtgaagct gaaggtgacc aagggcggcc
     8281 ccctgccctt cgcctgggac atcctgtccc cccagttcca gtacggctcc aaggtgtacg
     8341 tgaagcaccc cgccgacatc cccgactaca agaagctgtc cttccccgag ggcttcaagt
     8401 gggagcgcgt gatgaacttc gaggacggcg gcgtggcgac cgtgacccag gactcctccc
     8461 tgcaggacgg ctgcttcatc tacaaggtga agttcatcgg cgtgaacttc ccctccgacg
     8521 gccccgtgat gcagaagaag acgatgggct gggaggcctc caccgagcgc ctgtaccccc
     8581 gcgacggcgt gctgaagggc gagacacaca aggccctgaa gctgaaggac ggcggccact
     8641 acctggtgga gttcaagtcc atctacatgg ccaagaagcc cgtgcagctg cccggctact
     8701 actacgtgga cgccaagctg gacatcacct cccacaacga ggactacacc atcgtggagc
     8761 agtacgagcg caccgagggc cgccaccacc tgttcctgta ggcttcggcc atgctagagt
     8821 ccgcaaaaat caccagtctc tctctacaaa tctatctctc tctatttttc tccagaataa
     8881 tgtgtgagta gttcccagat aagggaatta gggttcttat agggtttcgc tcatgtgttg
     8941 agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt
     9001 tctaattcct aaaaccaaaa tccagtgacc tcgctgtcag gagtctcaat ggtaacttta
     9061 ctctttattt aaccatacat ttttttttat ttttttcact ttgttcttca tccactattg
     9121 ttctttgttc atcttgaaca aaagctccct ccttctttgt tcttcatcca ccattgttct
     9181 tcatcaatca tttcgctgtc atgagacgaa ttctgacagg atatattggc gggtaaacct
     9241 aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa aaggtttatc
     9301 cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc aaagtacttt
     9361 gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtcatc
     9421 tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc
     9481 ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg
     9541 gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg cccgcgtcag
     9601 caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa
     9661 gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct
     9721 tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag
     9781 cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag
     9841 cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt
     9901 cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga
     9961 ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat
    10021 cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact
    10081 gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc
    10141 caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct
    10201 ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag
    10261 gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg
    10321 ttcgagccgc ccgcgcacct ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct
    10381 gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga gcgccgccgt
    10441 ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg
    10501 atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta
    10561 accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac
    10621 tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg
    10681 cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc
    10741 gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg
    10801 cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa
    10861 gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg
    10921 tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca
    10981 tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta
    11041 tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag
    11101 aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac
    11161 tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg
    11221 ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc
    11281 catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc
    11341 ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga
    11401 gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg
    11461 aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg
    11521 gttggccagg cgtaagcggc tgggttgcct gccggccctg caatggcact ggaaccccca
    11581 agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc
    11641 gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat
    11701 cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga
    11761 atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga
    11821 gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat
    11881 catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg
    11941 ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt
    12001 gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata
    12061 ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact
    12121 caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat
    12181 tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct
    12241 ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac
    12301 cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg agatcacaga
    12361 aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat
    12421 cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt
    12481 gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac
    12541 cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg
    12601 gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc
    12661 cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg
    12721 aaaaggactc tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa
    12781 ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt
    12841 gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa
    12901 aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct
    12961 gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc
    13021 tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg
    13081 cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca ccctgcctcg
    13141 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggtg acggtcacag
    13201 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
    13261 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
    13321 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
    13381 gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
    13441 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
    13501 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
    13561 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    13621 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    13681 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    13741 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
    13801 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    13861 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    13921 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    13981 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    14041 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    14101 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    14161 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    14221 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta ggtgattaga
    14281 aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat
    14341 atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga
    14401 tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta
    14461 atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat
    14521 ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca ggccagccat
    14581 tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct
    14641 gagcgagtcg aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca
    14701 accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt
    14761 ctaatacctg gaatgctgtt ttccctggga tcgcagtggt gagtaaccat gcatcatcag
    14821 gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc
    14881 tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact
    14941 ctggcgcatc gggcttccca tacaatcggt agattgtcgc acctgattgc ccgacattat
    15001 cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat cgcggccttg
    15061 agcaagacgt ttcccgttga atatggctca taacagaact tattatttcc ttcctctttt
    15121 ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc caattcactg
    15181 ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag
    15241 ttggcgtata acatagtatc gacggagccg attttgaaac cgcggtgatc acaggcagca
    15301 acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac
    15361 ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc
    15421 ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg
    15481 attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg
    15541 gtgtaaacat aacgaattcg tctca
//
