LOCUS       sequence-322052-        4100 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  chimeric construct comprising the N-terminal fragment of A. thaliana
            PhyB (amino acids 1-650) fused to the VP16 activation domain and a 
            nuclear localization sequence. Part of a red light-controlled 
            synthetic genetic switch..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4100)
  TITLE     Orzaez lab plasmids
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUPD2 PhyB (GB2721)
FEATURES             Location/Qualifiers
     source          1..4100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(5..99)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(120..779)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     694..713
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     941..959
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(963..1006)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1009..1030
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      3082..3139
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(3163..3180)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3334..3922)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3414..3433)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
       61 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
      121 tacgccccgc cctgccactc gtcgcagtac tgttgtaatt cattaagcat tctgccgaca
      181 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
      241 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
      301 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgatacgaa aaacatattc
      361 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
      421 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagtga tgaaaacgtt
      481 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
      541 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
      601 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
      661 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
      721 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
      781 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
      841 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga
      901 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca
      961 gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcgcggcc
     1021 gcttctagag cgatgagtgg tctcgaatga caacaatggt ttccggagtc gggggtagtg
     1081 gcggtggccg tggcggtggc cgtggcggag aagaagaacc gtcgtcaagt cacactccta
     1141 ataaccgaag aggaggagaa caagctcaat cgtcgggaac gaaatctctc agaccaagaa
     1201 gcaacactga atcaatgagc aaagcaattc aacagtacac cgtcgacgca agactccacg
     1261 ccgttttcga acaatccggc gaatcaggga aatcattcga ctactcacaa tcactcaaaa
     1321 cgacgacgta cggttcctct gtacctgagc aacagatcac agcttatctc tctcgaatcc
     1381 agcgaggtgg ttacattcag cctttcggat gtatgatcgc cgtcgatgaa tccagtttcc
     1441 ggatcatcgg ttacagtgaa aacgccagag aaatgttagg gattatgcct caatctgttc
     1501 ctactcttga gaaacctgag attctagcta tgggaactga tgtgagatct ttgttcactt
     1561 cttcgagctc gattctactc gagcgtgctt tcgttgctcg agagattacc ttgttaaatc
     1621 cggtttggat ccattccaag aatactggta aaccgtttta cgccattctt cataggattg
     1681 atgttggtgt tgttattgat ttagagccag ctagaactga agatcctgcg ctttctattg
     1741 ctggtgctgt tcaatcgcag aaactcgcgg ttcgtgcgat ttctcagtta caggctcttc
     1801 ctggtggaga tattaagctt ttgtgtgaca ctgtcgtgga aagtgtgagg gacttgactg
     1861 gttatgatcg tgttatggtt tataagtttc atgaagatga gcatggagaa gttgtagctg
     1921 agagtaaacg agatgattta gagccttata ttggactgca ttatcctgct actgatattc
     1981 ctcaagcgtc aaggttcttg tttaagcaga accgtgtccg aatgatagta gattgcaatg
     2041 ccacacctgt tcttgtggtc caggacgata ggctaactca gtctatgtgc ttggttggtt
     2101 ctactcttag ggctcctcat ggttgtcact ctcagtatat ggctaacatg ggatctattg
     2161 cgtctttagc aatggcggtt ataatcaatg gaaatgaaga tgatgggagc aatgtagcta
     2221 gtggaagaag ctcgatgagg ctttggggtt tggttgtttg ccatcacact tcttctcgct
     2281 gcataccgtt tccgctaagg tatgcttgtg agtttttgat gcaggctttc ggtttacagt
     2341 taaacatgga attgcagtta gctttgcaaa tgtcagagaa acgcgttttg agaacgcaga
     2401 cactgttatg tgatatgctt ctgcgtgact cgcctgctgg aattgttaca cagagtccca
     2461 gtatcatgga cttagtgaaa tgtgacggtg cagcatttct ttaccacggg aagtattacc
     2521 cgttgggtgt tgctcctagt gaagttcaga taaaagatgt tgtggagtgg ttgcttgcga
     2581 atcatgcgga ttcaaccgga ttaagcactg atagtttagg cgatgcgggg tatcccggtg
     2641 cagctgcgtt aggggatgct gtgtgcggta tggcagttgc atatatcaca aaaagagact
     2701 ttcttttttg gtttcgatct cacactgcga aagaaatcaa atggggaggc gctaagcatc
     2761 atccggagga taaagatgat gggcaacgaa tgcatcctcg ttcgtccttt caggcttttc
     2821 ttgaagttgt taagagccgg agtcagccat gggaaactgc ggaaatggat gcgattcact
     2881 cgctccagct tattctgaga gactctttta aagaatctga ggcggctatg aactctaaag
     2941 ttgtggatgg tgtggttcag ccatgtaggg atatggcggg ggaacagggg attgatgagt
     3001 taggtgaatt cgatagtgct ggtagtgctg gtagtgctgg ttcgtgagac cactcatcgc
     3061 gactagtagc ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt
     3121 ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct
     3181 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     3241 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     3301 caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga
     3361 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     3421 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     3481 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     3541 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     3601 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     3661 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     3721 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     3781 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     3841 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3901 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     3961 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     4021 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     4081 ttggtctgac agctcgaggc
//
