LOCUS       sequence-322067-        2783 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Copper dependent transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2783)
  TITLE     Orzaez lab plasmids
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUPD2 CUP2 (GB2716)
FEATURES             Location/Qualifiers
     source          1..2783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(90..749)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     664..683
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     911..929
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(933..976)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    979..1000
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1735..1792
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1816..1833)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1987..2575)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2067..2086)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(join(2758..2783,1..69))
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
       61 acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc gtcgcagtac
      121 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
      181 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
      241 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
      301 cagggattgg ctgatacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
      361 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
      421 tggtattcac tccagagtga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
      481 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga
      541 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
      601 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
      661 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
      721 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
      781 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
      841 acgtgcccga tcaactcgag tgccacctga cgtctaagaa accattatta tcatgacatt
      901 aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt
      961 cgctaaggat gatttctgga attcgcggcc gcttctagag cgatgagtgg tctcgaatgg
     1021 tcgtaattaa cggggtcaaa tatgcctgtg aaacgtgtat caggggtcac agggcggcgc
     1081 agtgtactca cactgatggt ccgctacaga tgatcagacg caagggaaga ccatcgacca
     1141 catgtggcca ttgtaaagag ctgagaagaa ccaagaactt caacccatcc ggtgggtgca
     1201 tgtgtgcctc tgcacgacgg ccagctgttg gcagcaagga agatgaaaca cgatgtcgtt
     1261 gtgatgaggg tgaaccttgt aaatgtcata ccaagaggaa aagcagccgg aaatcaaagg
     1321 gagggtcatg ccacagaagg gcaaatgatg aagcagcgca tgtcaatggt cttggtattg
     1381 cagatctgga cgttcttttg ggcctaaatg gtcgctcgtc ggatgtagac atgacaacca
     1441 cattgccgag tttgaagcca cctctgcaaa acggagaaat taaggccgac agcattgaca
     1501 atcttgattt ggcttccctc gatccgcttg agcaaagccc tagtatatct atggaacctg
     1561 ttagtatcaa tgaaacagga agcgcatata caactacgaa cacagcacta aacgatattg
     1621 acattccatt ctccatcaat gagttgaacg agctatacaa acaagtatct tcgcataact
     1681 cacattcaca gggttcgtga gaccactcat cgcgactagt agcggccgct gcagtccggc
     1741 aaaaaagggc aaggtgtcac caccctgccc tttttcttta aaaccgaaaa gattacttcg
     1801 cgttatgcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1861 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     1921 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     1981 ggcgtttttc cacaggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2041 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2101 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2161 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2221 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2281 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2341 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2401 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     2461 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2521 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2581 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     2641 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     2701 ttttaaatca atctaaagta tatatgagta aacttggtct gacagctcga ggcttggatt
     2761 ctcaccaata aaaaacgccc ggc
//
