LOCUS       sequence-322086-        2841 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Streptomyces phage PhiC31-encoded recombination directionality 
            factor (RDF) (PhiC31 gp3).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Bernabe-Orts JM, Quijano-Rubio A, Vazquez-Vilar M, Mancheno-Bonillo 
            J, Moles-Casas V, Selma S, Gianoglio S, Granell A, Orzaez D
  TITLE     A memory switch for plant synthetic biology based on the phage 
            varphiC31 integration system.
  JOURNAL   Nucleic Acids Res. 2020 Apr 6;48(6):3379-3394. doi: 
            10.1093/nar/gkaa104.
  PUBMED    32083668
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2020-04-6- 6"; volume: "48"; issue: "6";
            pages: "3379-3394"
COMMENT     Sequence Label: pUPD2 RDF ( GB1498)
FEATURES             Location/Qualifiers
     source          1..2841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      778..835
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(859..876)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1030..1618)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1110..1129)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1801..1895)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1916..2575)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2490..2509
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2737..2755
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2759..2802)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2805..2826
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatggctaag agatctattt gggctggtga tgaagataat aagcctaaga agagagaaac
       61 ttatgctgat gatactgttg gtagatttca ttctggttat tctgaaacta atgaaagagg
      121 taaggttgtt cctgttgctc ttgataagtg gagaatttct actggtgaac aatctgttgc
      181 tgatgctgtt gctcaacttt ttggtggtac tcctgttgaa aatgaagaat ctacttctga
      241 aaattttatt gatgttttta ctgatagacc taaggttcct gttattattg aagctgatgg
      301 tattcattgg gatatgaagc tttggcttaa tggtaagctt aagcatcatt gtgatggttt
      361 tgattttgtt tctcatgctg atgaagaaat gattggtcaa ccttgtggtt gtcctaagct
      421 ttttgatgaa agaaaggctg ctgctaagga atatgatgct cctaatcctg ctattactgt
      481 tacttttact cttgctgatg atcctgaact tggtagattt aagtttcaaa ctggttcttg
      541 gactcttttt aaggttcttc atgaagctga agatgatgtt gaaagagttg gtaagggtgg
      601 tgctgttctt gctaatcttg aacttgaact tgttgaatat actcctaaga gaggtcctat
      661 gagaaataag cttgtttctt attataagcc tactattact gttcttaagt cttataatga
      721 tgctattgct gattaggctt tgagaccact catcgcgact agtagcggcc gctgcagtcc
      781 ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact
      841 tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      901 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
      961 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     1021 gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1081 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1141 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1201 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1261 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1321 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1381 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1441 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     1501 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1561 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1621 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     1681 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     1741 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct cgaggcttgg
     1801 attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
     1861 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
     1921 ccccgccctg ccactcgtcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
     1981 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
     2041 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
     2101 ttaaatcaaa actggtgaaa ctcacccagg gattggctga tacgaaaaac atattctcaa
     2161 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
     2221 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagtgatgaa aacgtttcag
     2281 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
     2341 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
     2401 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
     2461 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
     2521 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
     2581 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     2641 cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc
     2701 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat
     2761 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc gcggccgctt
     2821 ctagagcgat gagtggtctc g
//
