LOCUS       sequence-32244-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Wang Y, Stary JM, Wilhelm JE, Newmark PA
  TITLE     A functional genomic screen in planarians identifies novel 
            regulators of germ cell development.
  JOURNAL   Genes Dev. 2010 Sep 15. 24(18):2081-92.
  PUBMED    20844018
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 Sep 15. 24(18):2081-92."
COMMENT     Sequence Label: pBS-NF-YB
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(686..706)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(686..704)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(725..741)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(725..741)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(738..760)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    749..765
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(773..779,780..797,798..803))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    818..839
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggtatcgata aagcttgata tcgaattcca ttgtgttggg cagattcctg ccgatttgca
       61 acgtttcgaa aataatgaaa aaggatcttc cgttcagtgc aaagatagcc aaggatgcta
      121 aacagtgcgt tcaagaatgc gcatcggaat tcatcagctt tgtgagcagc gaagcagcag
      181 aaatctgtca aaatgacaaa aggaaaacaa ttaatggaga agatattttg caagcttttg
      241 ccaatttggg atttgataat tacgtggaga ccttgcagaa ctttttgcaa acttatagag
      301 aggcaaataa atttgaaaat gatatcattg acctgggcaa caataataat gcgcaaggca
      361 ggccttggtc aatggcgcca gtaaactcaa actcgtcatc agaactgaat atcaaactga
      421 acatgcctca tctgctttcc atgctcccgt tgaccgacct cacctcgagc aatctgaatt
      481 tggaaaatct tcttcgtgat tcagatttcc aaagagcagt tttgaatcac ttgaagaaat
      541 catcaaccga tgaagactcc tttaatatgg atgaagcatt ataattatta tttttattgt
      601 tcaaattata tattttttgg attcttaaaa aaaaaaaaaa aaaatgcgag cggccgccac
      661 cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
      721 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
      781 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
      841 ttgcgttgcg ctcactgccc gctttccagt cgggaa
//
