LOCUS       sequence-322495-        7876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses in Candida albicans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7876)
  AUTHORS   Van Genechten W, Demuyser L, Dedecker P, Van Dijck P
  TITLE     Presenting a codon-optimized palette of fluorescent proteins for use
            in Candida albicans.
  JOURNAL   Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.1038/s41598-020-63308-w.
  PUBMED    32273559
REFERENCE   2  (bases 1 to 7876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-04-9"; volume: "10(1):6158. doi"; pages: " 
            10.1038/s41598-020-63308-w"
COMMENT     Sequence Label: CIp10 - YemVenus (OPT High)
FEATURES             Location/Qualifiers
     source          1..7876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(727..743)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(728..744)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(1153..1171)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1192..1208)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1192..1208)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1205..1227)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1216..1232
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1240..1246,1247..1264,1265..1270))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1285..1306
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1423..1440)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1594..2182)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1674..1693)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2353..3144,3145..3213))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2976..2995
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3214..3318)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3345..3800
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3432..3451)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3642..3663
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3926..3948
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3940..3957
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3941..3957
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3964..3983
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3964..3982
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atgtctaagg gtgaagaatt gtttaccggt gtggtcccca tcttggttga gttagacggt
       61 gatgttaacg ggcataagtt cagcgtatca ggtgaaggtg aaggtgacgc gacctacggt
      121 aagttgactc taaaattaat ttgtaccacg ggtaaattgc cagttccatg gccaactcta
      181 gttaccacgt tgggttacgg tttacagtgc tttgccagat acccagacca catgaaacaa
      241 cacgacttct ttaaatctgc catgccggaa ggttacgtac aagagagaac cattttcttc
      301 aaagatgatg gtaattataa gacgagagcg gaagtcaagt tcgaaggtga tactttggtt
      361 aacagaattg aattaaaagg catcgacttc aaagaggacg ggaacattct aggtcataag
      421 ttggaataca attacaatct gcataacgtt tacattactg ccgacaaaca aaaaaacggc
      481 attaaggcca acttcaaaat tagacacaac attgaagatg ggggtgttca actagccgac
      541 cactaccagc aaaatactcc aattggtgac gggccagttt tgttgccaga caaccattac
      601 ttgtcatatc aatccaaatt atcaaaggac ccaaacgaga aacgcgatca catggttttg
      661 ctagaatttg ttaccgctgc cggtattacg catggtatgg atgaattata caaataacga
      721 cgtcatcgat accgtcgacc tcgaggagtg aaattctgga aatctggaaa tctggttttg
      781 tattcttgtt attcttcttt ttgttattac atatataact tgttactttt ttaaaaaaat
      841 ctttgtttat tttataaata tataaaacta aatttaagaa aaagagaaaa atgttttatt
      901 tgagagattg aaattttact tgaatttagc ttagctttta taaagtatta ttatgtaaaa
      961 aaacaaaaca aatatacatt aaaaagttaa gactataaaa tagccaccca aggcatttct
     1021 atatcttgtt gttgttgttt tcatcttctg tatcagagga acttatttta ttattttcgt
     1081 cacgggtatt ttctcttgtt tgatgattca tcccattcat tccatcaaaa atgtcggtac
     1141 cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat ggtcatagct
     1201 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     1261 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     1321 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     1381 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     1441 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     1501 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     1561 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     1621 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1681 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1741 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1801 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1861 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1921 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1981 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     2041 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     2101 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     2161 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     2221 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     2281 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     2341 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     2401 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     2461 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     2521 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     2581 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     2641 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     2701 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     2761 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     2821 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     2881 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     2941 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     3001 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     3061 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     3121 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     3181 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     3241 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     3301 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg
     3361 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     3421 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     3481 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     3541 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     3601 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     3661 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     3721 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     3781 aatattaacg cttacaattt ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     3841 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     3901 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
     3961 ttgtaatacg actcactata gggcgaattg gagctccacc gcggtggcgg ccgcgtttat
     4021 aataaactta gtctaatatt tcttttggac caaaatagaa actgttctca ggtggcaaaa
     4081 aacagcttaa agtctgtgtt aaaaaaagat ctcggctatg ccaatcaaaa aggagtgaca
     4141 ttattatgta aaagaagaaa tgttgtaatt ccgcgaaaca acaattttaa tgcttactgt
     4201 gccgccacca atatcctatg gccaaggggc ctttaaacat gccacggaaa tatacagtca
     4261 aagtaaacct actatgtctt tagagccttc aggatcaatt gtttttaaat gaatgactgc
     4321 aaaactagaa aagtagcctc ttacataagc aagtcatttg tacaccgaga gtttagtaac
     4381 cagtgaagca agtatatctg gcaaacctga aacttatctg tgaattataa agctcataag
     4441 taaaaagtgt agaaaagaag ataaggcaga ccaaatacaa atttgcaaca gtacaacgaa
     4501 tatttcagcg tcaaaactag agaataataa agaaaacgat cttttcaaaa agaaaaaacc
     4561 ttttagtttt cctttgttgt tgttgtgggt gtgtgctatt tatattatat agtttactca
     4621 taataccata aaatattcgg tttgattagg ttattttaat aagctaattt gtttctaatc
     4681 gtgtaattta tgctgtgtat attaagtagt gtgtgcactg cccaaaaatg tttgttgttt
     4741 atagtcggtt aaagagaaaa aagaaaaaaa gatccataca cacacgttaa ttagttgttc
     4801 aacgtaatac actcatattt tgttcttatt tgctttcggt cgctgttctc accaagattt
     4861 attgccaacg aaacaatttt tttttatata ttttcagatt tttctttttt tcctttcctt
     4921 tccttttcta attttcactc ctggttttct ttctttctta gaaacattat ctcgatatta
     4981 atattaaaaa aatataatca ttcaaactcc ggaatgtcta ctactttgga tgatactgct
     5041 tatagataca gaacttctgt tccaggtgat gctgaagcta ttgaagcttt ggatggttct
     5101 ttcactaccg atactgtttt tagagttact gctactggtg atggtttcac tttgagagaa
     5161 gttccagttg atccaccatt gactaaggtt ttcccagatg atgaatccga tgatgaatcc
     5221 gatgctggtg aagatggtga tccagattct agaactttcg ttgcttatgg tgatgatggt
     5281 gatttggctg gtttcgttgt tgtttcttat tctggttgga acagaagatt gactgttgaa
     5341 gatattgaag ttgctccaga acatagaggt catggtgttg gtagagcttt gatgggtttg
     5401 gctactgaat tcgccagaga aagaggtgct ggtcatttgt ggttggaagt taccaatgtt
     5461 aatgctccag ctattcatgc ttatagaaga atgggtttca ctttgtgtgg tttggatact
     5521 gctttatacg atggtactgc ttccgatggt gaacaagctt tgtatatgtc tatgccatgt
     5581 ccataataac tcggggggat ccgagtgaaa ttctggaaat ctggaaatct ggttttgtat
     5641 tcttgttatt cttctttttg ttattacata tataacttgt tactttttta aaaaaatctt
     5701 tgtttatttt ataaatatat aaaactaaat ttaagaaaaa gagaaaaatg ttttatttga
     5761 gagattgaaa ttttacttga atttagctta gcttttataa agtattatta tgtaaaaaaa
     5821 caaaacaaat atacattaaa aagttaagac tataaaatag ccacccaagg catttctata
     5881 tcttgttgtt gttgttttca tcttctgtat cagaggaact tattttatta ttttcgtcac
     5941 gggtattttc tcttgtttga tgattcatcc cattcttcca tcataaaata ctagtggatc
     6001 ccccagatcc ttatccttaa acagattcta aaacaacatc tttgaaacca gaagaaactt
     6061 tcttaccttt ttcttcggtt gaaccttcac cgtgcaaagc caataatgaa cccaagtcga
     6121 attttggttg tttcaacaat ttgacttttc tgatgtggac attttgtaat gggaaaatgg
     6181 tttgggtgga tttttcaatt tcacggccaa tgacttctgg aatcaatttg gaagttaatt
     6241 gagctaaagt acagttggaa acttctcttt gcataatttc aatcatcttc tttctgactt
     6301 ctctcaattt agaggattga gcgtaagtag ttttcttgat ttggtttggt tgtcttttgg
     6361 tgaaagcaat ggcaaaaact ctcaaaacgt aatcgtcgga agttttaaca gtgacattag
     6421 cttcaactaa tgattgccat tttctgacca atgatcttaa tttgtcagaa gtgaaatcca
     6481 aaccatgagg cctccatgag gcctccatga ggcctccatg aggcctccat ggaggcctca
     6541 tggaagttgg tcaacaagtt tttaccttgg acttcatcaa ctctcaattt gatttttctg
     6601 taagagtggt cttcggaacc ttgtaagtcg gccaaacaaa cttcgaaaac tctacctttc
     6661 aagccatcag cggcattctt taaaccggta gatctgttga tcaaagtttt accaacattt
     6721 ctgttttcaa aagtggttgg agctttgatg tcaaaccaat cttttctggt gaatgggtca
     6781 acgaccttct tttttaatcc tttctttcct ttggacaatc tcttgttttt accgacagcc
     6841 atgttgtact tgagttggat ctacgcgtca gcctcgttta taataaactt agtctaatat
     6901 ttcttttgga ccaaaataga aactgttctc aggtggcaaa aaacagctta aagtctgtgt
     6961 taaaaaaaga tctcggctat gccaatcaaa aaggagtgac attattatgt aaaagaagaa
     7021 atgttgtaat tccgcgaaac aacaatttta atgcttactg tgccgccacc aatatcctat
     7081 ggccaagggg cctttaaaca tgccacggaa atatacagtc aaagtaaacc tactatgtct
     7141 ttagagcctt caggatcaat tgtttttaaa tgaatgactg caaaactaga aaagtagcct
     7201 cttacataag caagtcattt gtacaccgag agtttagtaa ccagtgaagc aagtatatct
     7261 ggcaaacctg aaacttatct gtgaattata aagctcataa gtaaaaagtg tagaaaagaa
     7321 gataaggcag accaaataca aatttgcaac agtacaacga atatttcagc gtcaaaacta
     7381 gagaataata aagaaaacga tcttttcaaa aagaaaaaac cttttagttt tcctttgttg
     7441 ttgttgtggg tgtgtgctat ttatattata tagtttactc ataataccat aaaatattcg
     7501 gtttgattag gttattttaa taagctaatt tgtttctaat cgtgtaattt atgctgtgta
     7561 tattaagtag tgtgtgcact gcccaaaaat gtttgttgtt tatagtcggt taaagagaaa
     7621 aaagaaaaaa agatccatac acacacgtta attagttgtt caacgtaata cactcatatt
     7681 ttgttcttat ttgctttcgg tcgctgttct caccaagatt tattgccaac gaaacaattt
     7741 ttttttatat attttcagat ttttcttttt ttcctttcct ttccttttct aattttcact
     7801 cctggttttc tttctttctt agaaacatta tctcgatatt aatattaaaa aaatataatc
     7861 attcaaaatg ctgcac
//
