LOCUS       sequence-32266-e         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Giordano-Santini R, Milstein S, Svrzikapa N, Tu D, Johnsen R, 
            Baillie D, Vidal M, Dupuy D
  TITLE     An antibiotic selection marker for nematode transgenesis.
  JOURNAL   Nat Methods. 2010 Sep;7(9):721-3. doi: 10.1038/nmeth.1494. Epub 2010
            Aug 22.
  PUBMED    20729841
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1494"; journalName: "Nat Methods"; date: "2010-09"; 
            volume: "7"; issue: "9"; pages: "721-3"
COMMENT     Sequence Label: pdestRG5273Neo
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     789..805
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     790..806
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gggctgacgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
       61 ctatcgcctt cttgacgagt tcttctgagt ccaattactc ttcaacatcc ctacatgctc
      121 tttctccctg tgctcccacc ccctattttt gttattatca aaaaacttct cttaatttct
      181 ttgtttttta gcttctttta agccacctct aacaatgaaa ttgtgtagat tcaaaaatag
      241 aattaattcg taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt aataataatt
      301 ctatcccaaa atctacacaa tgttctgtgt acacttctta tgttttttac ttctgataaa
      361 tttttttgaa acatcataga aaaaaccgca cacaaaatac cttatcatat gttacgtttc
      421 agtttatgac cgcaattttt atttcttcgc acgtctgggc ctctcatgac gtcaaatcat
      481 gctcatcgtg aaaaagtttt ggagtatttt tggaattttt caatcaagtg aaagtttatg
      541 aaattaattt tcctgctttt gctttttggg gtttccccta ttgtttgtca agatttcgag
      601 gacggcgttt ttcttgctaa aatcacaagt attgatgagc acgatgcaag aaagatcgga
      661 agaaggtttg ggtttgaggc tcagtggaag gtgagtagaa gttgataatt tgaaagtgga
      721 gtagtgtcta tggggttttt gccttaaatg acagaataca ttcccaatat accaaacata
      781 actgtttctc gaggtcgacg gtatcg
//
