LOCUS       sequence-32267-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Giordano-Santini R, Milstein S, Svrzikapa N, Tu D, Johnsen R, 
            Baillie D, Vidal M, Dupuy D
  TITLE     An antibiotic selection marker for nematode transgenesis.
  JOURNAL   Nat Methods. 2010 Sep;7(9):721-3. doi: 10.1038/nmeth.1494. Epub 2010
            Aug 22.
  PUBMED    20729841
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1494"; journalName: "Nat Methods"; date: "2010-09"; 
            volume: "7"; issue: "9"; pages: "721-3"
COMMENT     Sequence Label: pdestDD04Neo
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(44..64)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(73..172)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        329..431
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(498..517)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ctccagtgaa aagttcttct cctttactca ttttttctac cggtacctta cgcttcttct
       61 ttggagcagt caagcctgct tttttgtaca aagttggcat tataaaaaag cattgcttat
      121 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tggagctcta
      181 gagcgtcgac taagttggca gcatcacccg acgcactttg cgccgaataa atacctgtga
      241 cggaagatca cttcgcagaa taaataaatc ctggtgtccc tgttgatacc gggaagccct
      301 gggccaactt ttggcgaaaa tgagacgttg atcggcacgt aagaggttcc aactttcacc
      361 ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta
      421 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
      481 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
      541 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
      601 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaactc cgtatggcaa
      661 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
      721 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
      781 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
      841 g
//
