LOCUS       sequence-322822-        8134 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for curing pMUT2 in one step based on pFREE. Contains RelB 
            antitoxin, as well as gRNA targetting pMUT2 plasmid as well as 
            pCryptDel4.8 itself..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 3250 to 3284)
  AUTHORS   Kan A, Gelfat I, Emani S, Praveschotinunt P, Joshi NS
  TITLE     Plasmid Vectors for in Vivo Selection-Free Use with the Probiotic E.
            coli Nissle 1917.
  JOURNAL   ACS Synth Biol. 2020 Dec 10. doi: 10.1021/acssynbio.0c00466.
  PUBMED    33301298
REFERENCE   2  (bases 1 to 8134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Dec 10. doi: 10.1021/acssynbio.0c00466."
COMMENT     Sequence Label: pCryptDel4.8
FEATURES             Location/Qualifiers
     source          1..8134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             577..1200
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(650..669)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             complement(1362..2177)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1362..2177)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2085..2104
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(2178..2269)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2287..2922)
                     /direction=LEFT
                     /label=ColA ori
                     /note="Plasmids containing the ColA origin of replication 
                     can be propagated in E. coli cells that contain additional 
                     plasmids with compatible origins."
     primer_bind     complement(2432..2451)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_RNA        complement(3171..3249)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     promoter        complement(3250..3284)
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     repeat_region   3421..3456
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   3487..3522
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   3553..3588
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   3653..3688
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   3719..3754
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     protein_bind    3828..3846
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3853..3871
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             3957..8063
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTEEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(8098..8116)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      join(8112..8134,1..25)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 taaacgggtc ttgaggggtt ttttgctgaa acctcaggca tttgagaagc acacggtcac
       61 acttttgtca tccccgacaa tccgttattc ctgcttgttc tcgcacggct ttacgctcat
      121 actacttctt gtagatacac ttgtcactac atcaagaggt gagatgatgg ccacgattaa
      181 tattcggatc gatgacgagc tgaaaagccg ctcttatgcc gcactggaaa agctgggcgt
      241 aacgccgtcc gaggttctgc gccaaacact ggaatatgtg gcccaaagcg gacgtttgcc
      301 gttccagcag gttttgctga ccgaggatga tgccgatttg atggctatcg ttcgggatcg
      361 tctggaaaac ccacaggcgg gcgtaaaggt gtcactggat gagctataat agataactaa
      421 ctcggtacca aattccagaa aagaggcctc ccgaaagggg ggcctttttt cgttttggtc
      481 cgcttccggt agtcaataaa ccggtattga cgccaacggg tcacacgggt acagtgctag
      541 cagaaacata tccatgaaat cccgccccga attcatatgt ctagattaga taaaagtaaa
      601 gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt
      661 aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag
      721 cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc
      781 cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt
      841 gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa
      901 cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag
      961 aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat
     1021 caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca
     1081 ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc
     1141 ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtcttaa
     1201 gcaatagaca taagcggcta tttaacgacc ctgccctgaa ccgacgacaa gctgacgacc
     1261 gggtctccgc aagtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1321 tctaaataca ttcaaatatg tatccgctca tgaattaatt cttagaaaaa ctcatcgagc
     1381 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
     1441 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
     1501 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
     1561 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
     1621 aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
     1681 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat
     1741 acgcggtcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac
     1801 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat
     1861 gctgttttcc cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
     1921 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct
     1981 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
     2041 ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta
     2101 tacccatata aatcagcatc catgttggaa tttaatcgcg gcctagagca agacgtttcc
     2161 cgttgaatat ggctcatact cttccttttt caatattatt gaagcattta tcagggttat
     2221 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggcatgcta
     2281 gcgcagaaac gtcctagaag atgccaggag gatacttagc agagagacaa taaggccgga
     2341 gcgaagccgt ttttccatag gctccgcccc cctgacgaac atcacgaaat ctgacgctca
     2401 aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctgatggc
     2461 tccctcttgc gctctcctgt tcccgtcctg cggcgtccgt gttgtggtgg aggctttacc
     2521 caaatcacca cgtcccgttc cgtgtagaca gttcgctcca agctgggctg tgtgcaagaa
     2581 ccccccgttc agcccgactg ctgcgcctta tccggtaact atcatcttga gtccaacccg
     2641 gaaagacacg acaaaacgcc actggcagca gccattggta actgagaatt agtggattta
     2701 gatatcgaga gtcttgaagt ggtggcctaa cagaggctac actgaaagga cagtatttgg
     2761 tatctgcgct ccactaaagc cagttaccag gttaagcagt tccccaactg acttaacctt
     2821 cgatcaaacc gcctccccag gcggtttttt cgtttacaga gcaggagatt acgacgatcg
     2881 taaaaggatc tcaagaagat cctttacgga ttcccgacac catcactcta gatttcagtg
     2941 caatttatct cttcaaatgt agcacctgaa gtcagcccca tacgatataa gttgtaattc
     3001 tcatgttagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctatcaagg
     3061 gcatcggtcg agatcccggt gcctaatgag tgagctaact tacattaatt gcgttgcgca
     3121 caccgactag cgaaaaaacc ccgccgaagc ggggtttttt gcgaaaaaaa gcaccgactc
     3181 ggtgccactt tttcaagttg ataacggact agccttattt taacttgcta tgctgttttg
     3241 aatggttccg ctagcactgt acctaggact gagctagccg tcaaaagcag cgtatatacc
     3301 atggccacaa ttcagcaaat tgtgaacatc atcacgttca tctttccctg gttgccaatg
     3361 gcccattttc ctgtcagtaa cgagaaggtc gcgaattcag gcgcttttta gactggtcgt
     3421 gttttagagc tatgctgttt tgaatggtcc caaaaccttg aatttgatcc ccgagccctg
     3481 aaggaagttt tagagctatg ctgttttgaa tggtcccaaa acgcccacac tcaccatcaa
     3541 aaaccccgag aagttttaga gctatgctgt tttgaatggt cccaaaacgg ttgagaccta
     3601 tatataggtc tcgtcccaaa acggttggac tcaagacgat agttaccgga tagttttaga
     3661 gctatgctgt tttgaatggt cccaaaacgg cgaaacccga caggactata aagataccgt
     3721 tttagagcta tgctgttttg aatggtccca aaacaaaaaa aaaccccgcc cctgacaggg
     3781 cggggttttt tttttaatta aagtctggtc ctcgagtctg gttataatcc ctatcagtga
     3841 tagagattga catccctatc agtgatagag atactgagca cagaaggaga tatacatgga
     3901 taagaaatac tgagcactat cagtgataga gatactgagc acagaaggag atatacatgg
     3961 ataagaaata ctcaataggc ttagatatcg gcacaaatag cgtcggatgg gcggtgatca
     4021 ctgatgaata taaggttccg tctaaaaagt tcaaggttct gggaaataca gaccgccaca
     4081 gtatcaaaaa aaatcttata ggggctcttt tatttgacag tggagagaca gcggaagcga
     4141 ctcgtctcaa acggacagct cgtagaaggt atacacgtcg gaagaatcgt atttgttatc
     4201 tacaggagat tttttcaaat gagatggcga aagtagatga tagtttcttt catcgacttg
     4261 aagagtcttt tttggtggaa gaagacaaga agcatgaacg tcatcctatt tttggaaata
     4321 tagtagatga agttgcttat catgagaaat atccaactat ctatcatctg cgaaaaaaat
     4381 tggtagattc tactgataaa gcggatttgc gcttaatcta tttggcctta gcgcatatga
     4441 ttaagtttcg tggtcatttt ttgattgagg gagatttaaa tcctgataat agtgatgtgg
     4501 acaaactatt tatccagttg gtacaaacct acaatcaatt atttgaagaa aaccctatta
     4561 acgcaagtgg agtagatgct aaagcgattc tttctgcacg attgagtaaa tcaagacgat
     4621 tagaaaatct cattgctcag ctccccggtg agaagaaaaa tggcttattt gggaatctca
     4681 ttgctttgtc attgggtttg acccctaatt ttaaatcaaa ttttgatttg gcagaagatg
     4741 ctaaattaca gctttcaaaa gatacttacg atgatgattt agataattta ttggcgcaaa
     4801 ttggagatca atatgctgat ttgtttttgg cagctaagaa tttatcagat gctattttac
     4861 tttcagatat cctaagagta aatactgaaa taactaaggc tcccctatca gcttcaatga
     4921 ttaaacgcta cgatgaacat catcaagact tgactctttt aaaagcttta gttcgacaac
     4981 aacttccaga aaagtataaa gaaatctttt ttgatcaatc aaaaaacgga tatgcaggtt
     5041 atattgatgg gggagctagc caagaagaat tttataaatt tatcaaacca attttagaaa
     5101 aaatggatgg tactgaggaa ttattggtga aactaaatcg tgaagatttg ctgcgcaagc
     5161 aacggacctt tgacaacggc tctattcccc atcaaattca cttgggtgag ctgcatgcta
     5221 ttttgagaag acaagaagac ttttatccat ttttaaaaga caatcgtgag aagattgaaa
     5281 aaatcttgac ttttcgaatt ccttattatg ttggtccatt ggcgcgtggc aatagtcgtt
     5341 ttgcatggat gactcggaag tctgaagaaa caattacccc atggaatttt gaagaagttg
     5401 tcgataaagg tgcttcagct caatcattta ttgaacgcat gacaaacttt gataaaaatc
     5461 ttccaaatga aaaagtacta ccaaaacata gtttgcttta tgagtatttt acggtttata
     5521 acgaattgac aaaggtcaaa tatgttactg aaggaatgcg aaaaccagca tttctttcag
     5581 gtgaacagaa gaaagccatt gttgatttac tcttcaaaac aaatcgaaaa gtaaccgtta
     5641 agcaattaaa agaagattat ttcaaaaaaa tagaatgttt tgatagtgtt gaaatttcag
     5701 gagttgaaga tagatttaat gcttcattag gtacctacca tgatttgcta aaaattatta
     5761 aagataaaga ttttttggat aatgaagaaa atgaagatat cttagaggat attgttttaa
     5821 cattgacctt atttgaagat agggagatga ttgaggaaag acttaaaaca tatgctcacc
     5881 tctttgatga taaggtgatg aaacagctta aacgtcgccg ttatactggt tggggacgtt
     5941 tgtctcgaaa attgattaat ggtattaggg ataagcaatc tggcaaaaca atattagatt
     6001 ttttgaaatc agatggtttt gccaatcgca attttatgca gctgatccat gatgatagtt
     6061 tgacatttaa agaagacatt caaaaagcac aagtgtctgg acaaggcgat agtttacatg
     6121 aacatattgc aaatttagct ggtagccctg ctattaaaaa aggtatttta cagactgtaa
     6181 aagttgttga tgaattggtc aaagtaatgg ggcggcataa gccagaaaat atcgttattg
     6241 aaatggcacg tgaaaatcag acaactcaaa agggccagaa aaattcgcga gagcgtatga
     6301 aacgaatcga agaaggtatc aaagaattag gaagtcagat tcttaaagag catcctgttg
     6361 aaaatactca attgcaaaat gaaaagctct atctctatta tctccaaaat ggaagagaca
     6421 tgtatgtgga ccaagaatta gatattaatc gtttaagtga ttatgatgtc gatcacattg
     6481 ttccacaaag tttccttaaa gacgattcaa tagacaataa ggtcttaacg cgttctgata
     6541 aaaatcgtgg taaatcggat aacgttccaa gtgaagaagt agtcaaaaag atgaaaaact
     6601 attggagaca acttctaaac gccaagttaa tcactcaacg taagtttgat aatttaacga
     6661 aagctgaacg tggaggtttg agtgaacttg ataaagctgg ttttatcaaa cgccaattgg
     6721 ttgaaactcg ccaaatcact aagcatgtgg cacaaatttt ggatagtcgc atgaatacta
     6781 aatacgatga aaatgataaa cttattcgag aggttaaagt gattacctta aaatctaaat
     6841 tagtttctga cttccgaaaa gatttccaat tctataaagt acgtgagatt aacaattacc
     6901 atcatgccca tgatgcgtat ctaaatgccg tcgttggaac tgctttgatt aagaaatatc
     6961 caaaacttga atcggagttt gtctatggtg attataaagt ttatgatgtt cgtaaaatga
     7021 ttgctaagtc tgagcaagaa ataggcaaag caaccgcaaa atatttcttt tactctaata
     7081 tcatgaactt cttcaaaaca gaaattacac ttgcaaatgg agagattcgc aaacgccctc
     7141 taatcgaaac taatggggaa actggagaaa ttgtctggga taaagggcga gattttgcca
     7201 cagtgcgcaa agtattgtcc atgccccaag tcaatattgt caagaaaaca gaagtacaga
     7261 caggcggatt ctccaaggag tcaattttac caaaaagaaa ttcggacaag cttattgctc
     7321 gtaaaaaaga ctgggatcca aaaaaatatg gtggttttga tagtccaacg gtagcttatt
     7381 cagtcctagt ggttgctaag gtggaaaaag ggaaatcgaa gaagttaaaa tccgttaaag
     7441 agttactagg gatcacaatt atggaaagaa gttcctttga aaaaaatccg attgactttt
     7501 tagaagctaa aggatataag gaagttaaaa aagacttaat cattaaacta cctaaatata
     7561 gtctttttga gttagaaaac ggtcgtaaac ggatgctggc tagtgccgga gaattacaaa
     7621 aaggaaatga gctggctctg ccaagcaaat atgtgaattt tttatattta gctagtcatt
     7681 atgaaaagtt gaagggtagt ccagaagata acgaacaaaa acaattgttt gtggagcagc
     7741 ataagcatta tttagatgag attattgagc aaatcagtga attttctaag cgtgttattt
     7801 tagcagatgc caatttagat aaagttctta gtgcatataa caaacataga gacaaaccaa
     7861 tacgtgaaca agcagaaaat attattcatt tatttacgtt gacgaatctt ggagctcccg
     7921 ctgcttttaa atattttgat acaacaattg atcgtaaacg atatacgtct acagaagaag
     7981 ttttagatgc cactcttatc catcaatcca tcactggtct ttatgaaaca cgcattgatt
     8041 tgagtcagct aggaggtgac tgagatccca tgttaccggt atcctaggct gctgccaccg
     8101 ctgagcaata actagcataa ccccttgggg cctc
//
