LOCUS       sequence-322847-        1118 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses reversibly switchable A-kinase activity reporter with 
            EV-linker inactive T/A mutant (rsAKARev(T/A)) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Roebroek T, Vandenberg W, Sipieter F, Hugelier S, Stove C, Zhang J, 
            Dedecker P
  TITLE     Simultaneous readout of multiple FRET pairs using photochromism.
  JOURNAL   Nat Commun. 2021 Mar 31;12(1):2005. doi: 10.1038/s41467-021-22043-0.
  PUBMED    33790271
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-31"; volume: "12(1):2005. doi"; pages: " 
            10.1038/s41467-021-22043-0"
COMMENT     Sequence Label: pcDNA3.1-rsAKARev(T/A)
FEATURES             Location/Qualifiers
     source          1..1118
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(96..117)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(297..644)
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
ORIGIN
        1 accccggtga acagctcctc gcccttgctc accatagatc caccggtacc tcccttgtac
       61 agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga
      121 tcgcgcttct cgttggggtc tttgctcagc ttggactggt agctcaggta gtggttgtcg
      181 ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc
      241 acgccgccca tgagctcgct gccgccgtca accagggccg cgcgcctcaa tccggaacca
      301 ccagcactac caccagcact accaccagca ctaccaccag cactaccacc agcactggta
      361 ctaccaccag cactaccacc agcactgccg gaaccaccag cactaccacc agcactacca
      421 ccagcactac caccagcact accaccagca ctggtactac caccagcact accaccagca
      481 ctgccggaac caccagcact accaccagca ctaccaccag cactaccacc agcactacca
      541 ccagcactgg tactaccacc agcactacca ccagcactgc cggaaccacc agcactacca
      601 ccagcactac caccagcact accaccagca ctaccaccag cactggtacc gcgatcaact
      661 ttgttctgct ccaggcactg cttaaatttg tcgtttatga aaatgaccag agataaaata
      721 tctgattcca cgcctacacc aacggttatt tcatcacctt gggacagtaa ctgattgctg
      781 ttcttctcga ctttttgccc atttaaccag gtcccattag tggaaatgtc attcaataaa
      841 aggttaccgt cttctcctag tagtatttgg aaatgcttat ttgacagtct tgaaatgttt
      901 cctaaatgat agtcacaggc tgggtttcta ccaaatgtcc aaactttctt tatggatcgt
      961 ttttccttaa gcacttgtga aatatcagct gacaaatctc ggatgggaat ttgacccgtg
     1021 gtacaaatga ccctgcacac aatgttttcg ccgatctgtt cttgagaaaa cttatgcata
     1081 gatctcttgt acagctcgtc catgccgccg gtggagta
//
