LOCUS       sequence-322848-        1084 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses reversibly switchable A-kinase activity reporter with 
            EV-linker inactive T/A mutant (rsAKARev(T/A)) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Roebroek T, Vandenberg W, Sipieter F, Hugelier S, Stove C, Zhang J, 
            Dedecker P
  TITLE     Simultaneous readout of multiple FRET pairs using photochromism.
  JOURNAL   Nat Commun. 2021 Mar 31;12(1):2005. doi: 10.1038/s41467-021-22043-0.
  PUBMED    33790271
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-31"; volume: "12(1):2005. doi"; pages: " 
            10.1038/s41467-021-22043-0"
COMMENT     Sequence Label: pcDNA3.1-rsAKARev(T/A)
FEATURES             Location/Qualifiers
     source          1..1084
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..761)
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     primer_bind     220..239
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     479..500
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(606..627)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgtgatggat atctgcagaa ttcttactcg atgttgtggc ggatcttgaa gttggccttg
       61 atgccgttct tctgcttgtc ggcggtgata tagacgttgt ggctgttgta gttgtactcc
      121 agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg
      181 ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc
      241 ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg
      301 tgctgcttca tgtggtcggg gtagcgggcg aagcactgaa ggccgtagcc cagggtggtc
      361 acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatcagctt cagggtcagc
      421 ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg
      481 tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc
      541 accatagatc caccggtacc tcccttgtac agctcgtcca tgccgagagt gatcccggcg
      601 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagc
      661 ttggactggt agctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg
      721 gtgttctgct ggtagtggtc ggcgagctgc acgccgccca tgagctcgct gccgccgtca
      781 accagggccg cgcgcctcaa tccggaacca ccagcactac caccagcact accaccagca
      841 ctaccaccag cactaccacc agcactggta ctaccaccag cactaccacc agcactgccg
      901 gaaccaccag cactaccacc agcactacca ccagcactac caccagcact accaccagca
      961 ctggtactac caccagcact accaccagca ctgccggaac caccagcact accaccagca
     1021 ctaccaccag cactaccacc agcactacca ccagcactgg tactaccacc agcactacca
     1081 ccag
//
