LOCUS       sequence-32303-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  TITLE     J.C. Anderson Lab Plasmids
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMLLKA-J72040
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(484..501)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(563..587)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        634..738
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cttgctagga ctgagtggat tcatgagatc tgaataccct actaaggaca ttcgccagaa
       61 aatgaccgac cgtctgaccc aactccaaat ttgcctggac cagatgaccg aacagttctg
      121 cgctaccctg aactacatcg acaaaaacca cggtttcgaa cgtctgaccg ttaacgaacc
      181 gcagatgtct gacaaacacg ctaccgttgt tccgccggaa gagttttcta acactattga
      241 cgaactctcc accgacatca tcctgaaaac ccgtcagatc aacaaactga tcgactctct
      301 gccaggcgtt gacgtttccg cagaggaaca actccgtaaa atcgacatgc tccaaaaaaa
      361 gctcgttgaa gttgaagacg aaaaaatcga agctatcaaa aaaaaagaaa aactgctgcg
      421 tcacgttgac tctctgatcg aagacttcgt tgacggtgga tcctaactcg ctcctcaggc
      481 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      541 ctcaaaggcg gtaatcaatt cgacccagct ttcttgtaca aagtggttga tcggatctct
      601 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
      661 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
      721 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
      781 gcggcatttt gccttcctgt tttttggctc acccagaaac gctggtgaaa
//
