LOCUS       sequence-323276-        2489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Omega1 4xLexAOp for assembly of larger LexAOp response elements.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: p4xLexAOp-O1
FEATURES             Location/Qualifiers
     source          1..2489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(456..1044)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(536..555)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1159..1261
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1262..1921
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1328..1347)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2034..2462
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2121..2140)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2331..2352
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taactgcagg tctcagtcag gaggcatgcg tcgactctag acccgggcag ctgcgctgtc
       61 aggagagctt gggctgcagg tcgaggctaa aaaactaatc gcattatcat cccctcgacg
      121 tactgtacat ataaccactg gttttatata cagcagtact gtacatataa ccactggttt
      181 tatatacagc agtcgacgta ctgtacatat aaccactggt tttatataca gcagtactgt
      241 acatataacc actggtttta tatacagcag tcgaggtaag attagatatg gatatgtata
      301 tggatatgta tatggtggta atgccatgta atatgccgct tgagacctgc agtgacacgg
      361 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      421 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      481 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      541 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      601 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      661 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      721 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      781 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      841 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
      901 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      961 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1021 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1081 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     1141 acctagatcc ttttgtgatg atcggcacgt aagaggttcc aactttcacc ataatgaaat
     1201 aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa
     1261 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
     1321 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
     1381 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
     1441 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg
     1501 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
     1561 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
     1621 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
     1681 gaatatgttt ttcgtgtcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
     1741 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
     1801 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca
     1861 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
     1921 atttttttaa ggcagttatt ggtgccctta aacgcctggt tgctacgcct gaataagtga
     1981 taataagcgg atgaatggca gaaattcgat gataagctgt caaacatgat gttacgcgcc
     2041 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     2101 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     2161 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     2221 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccagcgcc
     2281 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     2341 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     2401 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     2461 ttttaacaaa atattaacgc ttacaattt
//
