LOCUS       sequence-323637-        9977 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses soluble , dimeric Fc-His fusion protein for the original 
            SARS-CoV2 Spike RBD region, along with dTomato reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9977)
  AUTHORS   Yang Q, Hughes TA, Kelkar A, Yu X, Cheng K, Park S, Huang WC, Lovell
            JF, Neelamegham S
  TITLE     Inhibition of SARS-CoV-2 viral entry upon blocking N- and O-glycan 
            elaboration.
  JOURNAL   Elife. 2020 Oct 26;9. pii: 61552. doi: 10.7554/eLife.61552.
  PUBMED    33103998
REFERENCE   2  (bases 1 to 9977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-10-26"; volume: "9. pii: 61552. doi"; pages: " 
            10.7554/eLife.61552"
COMMENT     Sequence Label: RBD-Fc
FEATURES             Location/Qualifiers
     source          1..9977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        165..468
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        469..672
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             2126..2143
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2159..2215
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2222..2923
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(2355..2373)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2623..2642
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2817..2840
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             2930..2950
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2951..2968
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     LTR             3481..3661
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3687..3704)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3693..3917
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3963..4391
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4050..4069)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4260..4281
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4400..4420)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4405..4734
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4585..4720
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4647..4666
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4782..4829
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4848..5222
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5352..5473
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5389..5408)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5443..5462
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5522..5538)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5522..5538)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5535..5557)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5546..5562
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5570..5576,5577..5594,5595..5600))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5615..5636
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5753..5770)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5924..6512)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6004..6023)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6683..7474,7475..7543))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7306..7325
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7544..7648)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7723..7742)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7914..8293
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8295..8493
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8511..8691
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8738..8863
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9356..9589
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9774..9818
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
       61 gacagatcca ttcgattagt gaacggatcc cccgggctgc aggaattcga ttaatagtaa
      121 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      181 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      241 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      301 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
      361 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
      421 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      481 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      541 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      601 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      661 ataagcagag ctggtttagt gaaccgtcag atccgctagc gccgccacca tgattcctgc
      721 cagatttgcc ggggtgctgc ttgctctggc cctcattttg ccagggaccc tttgtaccgg
      781 tcgggtgcag cctaccgagt ccatcgtgcg ctttcccaac atcacaaacc tgtgcccttt
      841 tggagaggtg ttcaacgcta cccgcttcgc ctccgtgtac gcttggaacc ggaagcgcat
      901 ctccaactgc gtggccgact actctgtgct gtacaacagc gccagcttca gcaccttcaa
      961 gtgctacggc gtgagcccaa caaagctgaa cgacctgtgc tttaccaacg tgtacgctga
     1021 ttccttcgtg atcaggggag acgaggtgcg ccagatcgct cccggccaga caggaaagat
     1081 cgctgactac aactacaagc tgcctgacga tttcaccggc tgcgtgatcg cctggaactc
     1141 taacaacctg gatagcaaag tgggcggaaa ctacaactac ctgtacaggc tgtttagaaa
     1201 gtctaacctg aagccattcg agcgggacat ctccacagag atctaccagg ctggctctac
     1261 cccatgcaac ggagtggagg gcttcaactg ttacttccct ctgcagagct acggattcca
     1321 gccaacaaac ggcgtgggat accagcccta ccgcgtggtg gtgctgtctt ttgagctgct
     1381 gcacgctcct gctacagtgt gcggaccaaa gaagagcacc aacctggtga agaacaagtg
     1441 cgtgaacttc tctagaacat gcccaccgtg cccagcacct gaactcgcgg gggcaccgtc
     1501 agtcttcctc ttccccccaa aacccaagga caccctcatg atctcccgga cccctgaggt
     1561 cacatgcgtg gtggtggacg tgagccacga agaccctgag gtcaagttca actggtacgt
     1621 ggacggcgtg gaggtgcata atgccaagac aaagccgcgg gaggagcagt acaacagcac
     1681 gtaccgtgtg gtcagcgtcc tcaccgtcct gcaccaggac tggctgaatg gcaaggagta
     1741 caagtgcaag gtctccaaca aagccctccc agcccccatc gagaaaacca tctccaaagc
     1801 caaagggcag ccccgagaac cacaggtgta caccctgccc ccatcccggg atgagctgac
     1861 caagaaccag gtcagcctga cctgcctggt caaaggcttc tatcccagcg acatcgccgt
     1921 ggagtgggag agcaatgggc agccggagaa caactacaag accacgcctc ccgtgctgga
     1981 ctccgacggc tccttcttcc tctacagcaa gctcaccgtg gacaagagca ggtggcagca
     2041 ggggaacgtc ttctcatgct ccgtgatgca tgaggctctg cacaaccact acacgcagaa
     2101 gagcctctcc ctgtctccgg gtaaacacca tcatcatcat catttcgaag gcagcggcgc
     2161 caccaacttc agcctgctga agcaggccgg cgacgtggag gagaaccccg gccccgttaa
     2221 catggtgagc aagggcgagg aggtcatcaa agagttcatg cgcttcaagg tgcgcatgga
     2281 gggctccatg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     2341 gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga
     2401 catcctgtcc ccccagttca tgtacggctc caaggcgtac gtgaagcacc ccgccgacat
     2461 ccccgattac aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     2521 cgaggacggc ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat
     2581 ctacaaggtg aagatgcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa
     2641 gaccatgggc tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg
     2701 cgagatccac caggccctga agctgaagga cggcggccac tacctggtgg agttcaagac
     2761 catctacatg gccaagaagc ccgtgcaact gcccggctac tactacgtgg acaccaagct
     2821 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc gctccgaggg
     2881 ccgccaccac ctgttcctgt acggcatgga cgagctgtac aagtaagtcg aaaacctgta
     2941 cttccagggc catcaccatc accatcactg actcgagacc tagaaaaaca tggagcaatc
     3001 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
     3061 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca
     3121 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     3181 caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
     3241 tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac
     3301 aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc
     3361 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg
     3421 aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact
     3481 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     3541 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     3601 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     3661 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     3721 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     3781 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     3841 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     3901 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     3961 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     4021 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     4081 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     4141 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     4201 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     4261 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     4321 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     4381 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     4441 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     4501 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4561 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     4621 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     4681 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     4741 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
     4801 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga
     4861 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
     4921 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
     4981 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
     5041 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
     5101 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
     5161 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
     5221 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     5281 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     5341 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5401 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5461 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     5521 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     5581 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     5641 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     5701 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     5761 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     5821 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     5881 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     5941 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6001 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6061 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     6121 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6181 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6241 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     6301 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6361 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     6421 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     6481 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     6541 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     6601 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     6661 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     6721 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     6781 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     6841 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     6901 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     6961 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     7021 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     7081 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     7141 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     7201 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     7261 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     7321 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     7381 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     7441 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     7501 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     7561 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     7621 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     7681 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
     7741 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     7801 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     7861 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
     7921 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     7981 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     8041 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     8101 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     8161 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     8221 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     8281 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     8341 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     8401 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     8461 taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct
     8521 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     8581 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     8641 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     8701 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     8761 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     8821 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     8881 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     8941 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     9001 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     9061 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     9121 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     9181 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     9241 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     9301 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     9361 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     9421 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     9481 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     9541 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     9601 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     9661 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     9721 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     9781 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     9841 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     9901 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     9961 ttcagaccca cctccca
//
