LOCUS       sequence-323698-        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express mCerulean3 fused to the N-terminus of Bcl-2 family protein, 
            Mcl-1, in a plasmid compatable for lentiviral infection. Used to 
            make stable BMK-DKO cell lines..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Osterlund EJ, Hirmiz N, Pemberton JM, Nougarede A, Liu Q, Leber B, 
            Fang Q, Andrews DW
  TITLE     Efficacy and specificity of inhibitors of BCL-2 family protein 
            interactions assessed by affinity measurements in live cells.
  JOURNAL   Sci Adv. 2022 Apr 22;8(16):eabm7375. doi: 10.1126/sciadv.abm7375. 
            Epub 2022 Apr 20.
  PUBMED    35442739
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2022-04-22"; volume: "8(16):eabm7375. doi"; pages: " 
            10.1126/sciadv.abm7375. Epub 2022 Apr 20"
COMMENT     Sequence Label: mCerulean3-Mcl-1-pLVX
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tttggcctca aaagaaacgc ggtaatcgga ctcaacctct actgtggggg ggccggcttg
       61 ggggccggca gcggcggcgc cacccgcccg ggagggcgac ttttggctac ggagaaggag
      121 gcctcggccc ggcgagagat agggggaggg gaggccggcg cggtgattgg cggaagcgcc
      181 ggcgcaagcc ccccgtccac cctcacgcca gactcccgga gggtcgcgcg gccgccgccc
      241 attggcgccg aggtccccga cgtcaccgcg acccccgcga ggctgctttt cttcgcgccc
      301 acccgccgcg cggcgccgct tgaggagatg gaagccccgg ccgctgacgc catcatgtcg
      361 cccgaagagg agctggacgg gtacgagccg gagcctctcg ggaagcggcc ggctgtcctg
      421 ccgctgctgg agttggtcgg ggaatctggt aataacacca gtacggacgg gtcactaccc
      481 tcgacgccgc cgccagcaga ggaggaggag gacgagttgt accggcagtc gctggagatt
      541 atctctcggt accttcggga gcaggccacc ggcgccaagg acacaaagcc aatgggcagg
      601 tctggggcca ccagcaggaa ggcgctggag accttacgac gggttgggga tggcgtgcag
      661 cgcaaccacg agacggcctt ccaaggcatg cttcggaaac tggacatcaa aaacgaagac
      721 gatgtgaaat cgttgtctcg agtgatgatc catgttttca gcgacggcgt aacaaactgg
      781 ggcaggattg tgactctcat ttcttttggt gcctttgtgg ctaaacactt gaagaccata
      841 aaccaagaaa gctgcatcga accattagca gaaagtatca cagacgttct cgtaaggaca
      901 aaacgggact ggctagttaa acaaagaggc tgggatgggt ttgtggagtt cttccatgta
      961 gaggacctag aaggtggcat caggaatgtg ctgctggctt ttgcaggtgt tgctggagta
     1021 ggagctggtt tggcatatct aataagatga
//
