LOCUS       sequence-32370-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Paprotka K, Giese B, Fraunholz MJ
  TITLE     Codon-improved fluorescent proteins in investigation of 
            Staphylococcus aureus host pathogen interactions.
  JOURNAL   J Microbiol Methods. 2010 Oct;83(1):82-6. doi: 
            10.1016/j.mimet.2010.07.022. Epub 2010 Aug 11.
  PUBMED    20708040
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.mimet.2010.07.022"; journalName: "J Microbiol Methods"; 
            date: "2010-10"; volume: "83"; issue: "1"; pages: "82-6"
COMMENT     Sequence Label: pmRFPmars
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    19..37
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    104..122
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     CDS             856..873
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcctccttt ttgttgacac tctatcattg atagagttat ttgtcaaact agttttttat
       61 ttggatcccc tcgagttcat gaaaaactaa aaaaaatatt gacactctat cattgataga
      121 gtataattaa aataagcttg atggtaccag atcctaggaa aggaggatga taaaatggca
      181 tcatcagaag atgttattaa agaatttatg agattcaaag ttaaaatgga aggttcagtt
      241 aacggtcatg aatttgaaat tgaaggtgaa ggtgaaggta gaccatatga aggtacacaa
      301 acagcaaaat taaaagttac aaaaggtggt ccattaccat ttgcatggga tattctttca
      361 ccacaatttc aatatggtag taaagcatat gttaaacatc cagcagatat tccagattat
      421 cttaaacttt catttccaga aggttttaaa tgggaaagaa ttatgaattt tgaagatggt
      481 ggtgttgtaa cagttactca agattcaaca ttacaagatg gtgaatttat ttataaagtt
      541 aaattaagag gtacaaattt tccatcagat ggtccagtaa tgcaaaaaaa aactatgggt
      601 tgggaagcat caacagaaag aatgtatcca gaagatggtg cactaaaagg tgaaattaaa
      661 atgagattaa aacttaagga tggtggtcat tatgatgcag aagttaaaac aacttataaa
      721 gctaaaaaac aagttcaatt accaggtgct tataaaacag atattaaatt agatattatt
      781 tcacataatg aagattatac tattgttgaa caatatgaaa gagcagaagg tagacattca
      841 acaggtgcag gatcccatca tcaccaccat cattaa
//
