LOCUS       sequence-323939-        2306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 transcription terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Gale GAR, Wang B, McCormick AJ
  TITLE     Evaluation and Comparison of the Efficiency of Transcription 
            Terminators in Different Cyanobacterial Species.
  JOURNAL   Front Microbiol. 2021 Jan 15;11:624011. doi: 
            10.3389/fmicb.2020.624011. eCollection 2020.
  PUBMED    33519785
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2021-01-15"; volume: "11:624011. doi"; pages: " 
            10.3389/fmicb.2020.624011. eCollection 2020"
COMMENT     Sequence Label: pC0.294 L3S3P21
FEATURES             Location/Qualifiers
     source          1..2306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(147..735)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(227..246)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(906..1697)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1919..2023)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2091..2109
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2282..2299)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
       61 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
      121 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      181 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      241 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      301 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      361 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      421 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      481 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
      541 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
      601 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
      661 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
      721 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
      781 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
      841 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
      901 gacagttatt tgccgactac cttggtgatc tcgcctttta cgtaatggac aaattcttcc
      961 aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc ctgtctagct
     1021 tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca
     1081 tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact
     1141 acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt
     1201 agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct
     1261 accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc
     1321 gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt
     1381 tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct
     1441 acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc
     1501 aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatcg
     1561 ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc
     1621 ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg
     1681 atcaccgctt ccctcatgat gtttaacttt gttttagggc gactgccctg ctgcgtaaca
     1741 tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat
     1801 gcccgaggca tagactgtac cccaaaaaaa aacagtcata acaagccatg aaaaccgcca
     1861 ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac
     1921 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     1981 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     2041 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     2101 tcacgaggcc ctttcgtcca ctgaagagcc acttcgtggt ctcaagcggg gagaccagaa
     2161 acaaaaaaag gccccccgtt agggaggcct tcaataattg gaagctgaga ccacagagtg
     2221 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     2281 cctcgctcac tgactcgctg cgctcg
//
