LOCUS       sequence-323940-        2310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 transcription terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Gale GAR, Wang B, McCormick AJ
  TITLE     Evaluation and Comparison of the Efficiency of Transcription 
            Terminators in Different Cyanobacterial Species.
  JOURNAL   Front Microbiol. 2021 Jan 15;11:624011. doi: 
            10.3389/fmicb.2020.624011. eCollection 2020.
  PUBMED    33519785
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2021-01-15"; volume: "11:624011. doi"; pages: " 
            10.3389/fmicb.2020.624011. eCollection 2020"
COMMENT     Sequence Label: pC0.293 L3S2P55
FEATURES             Location/Qualifiers
     source          1..2310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(494..598)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     666..684
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(861..878)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1032..1620)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1112..1131)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1791..2310,1..272))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 acaatggtga cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc
       61 aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc
      121 agcaaatcaa tatcgctgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt
      181 acggccagca acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga
      241 gtcgatactt cggcgatcac cgcttccctc atgatgttta actttgtttt agggcgactg
      301 ccctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc
      361 gcttgctgct tggatgcccg aggcatagac tgtaccccaa aaaaaaacag tcataacaag
      421 ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt
      481 tgcgtgagcg catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
      541 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
      601 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
      661 ataaaaatag gcgtatcacg aggccctttc gtccactgaa gagccacttc gtggtctcaa
      721 gcgggaccaa aacgaaaaaa gacgcttttc agcgtcttat tgttcgtctt tggtaccgag
      781 aagctgagac cacagagtga ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
      841 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      901 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      961 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     1021 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     1081 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     1141 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     1201 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     1261 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1321 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1381 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     1441 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     1501 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     1561 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     1621 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     1681 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     1741 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttatttgccg
     1801 actaccttgg tgatctcgcc ttttacgtaa tggacaaatt cttccaactg atctgcgcgc
     1861 gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag tatgacgggc
     1921 tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt
     1981 ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg
     2041 ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga
     2101 tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta
     2161 tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag
     2221 atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga
     2281 taacgccacg gaatgatgtc gtcgtgcaca
//
