LOCUS       sequence-323942-        2304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 transcription terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Gale GAR, Wang B, McCormick AJ
  TITLE     Evaluation and Comparison of the Efficiency of Transcription 
            Terminators in Different Cyanobacterial Species.
  JOURNAL   Front Microbiol. 2021 Jan 15;11:624011. doi: 
            10.3389/fmicb.2020.624011. eCollection 2020.
  PUBMED    33519785
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2021-01-15"; volume: "11:624011. doi"; pages: " 
            10.3389/fmicb.2020.624011. eCollection 2020"
COMMENT     Sequence Label: pC0.295 L3S1P13
FEATURES             Location/Qualifiers
     source          1..2304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             126..917
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1088..1676
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1577..1596
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1830..1847
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2018..2036)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2104..2208
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
       61 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
      121 acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
      181 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
      241 atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc gtaaggcttg
      301 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
      361 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
      421 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
      481 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
      541 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
      601 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
      661 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
      721 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
      781 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
      841 tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc gagatcacca
      901 aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag attgatttaa
      961 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     1021 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1081 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1141 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1201 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     1261 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1321 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1381 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1441 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1501 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1561 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1621 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1681 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     1741 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     1801 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     1861 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatca ctctgtggtc
     1921 tcagcttgac gaacaataag gcctccctaa cggggggcct tttttattga taacaaaacg
     1981 cttgagacca cgaagtggct cttcagtgga cgaaagggcc tcgtgatacg cctattttta
     2041 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     2101 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     2161 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gcgctcacgc
     2221 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
     2281 gcttgttatg actgtttttt tttg
//
