LOCUS       sequence-32396-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pBPHLWL-GAL4DBD
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(279..312)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(497..515)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
ORIGIN
        1 gataccgtcg actaaagcca aatagaaaat tattcagttc ctggcttaag tttttaaaag
       61 tgatattatt tatttggttg taaccaacca aaagaatgta aataactaat acataattat
      121 gttagtttta agttagcaac aaattgattt tagctatatt agctacttgg ttaataaata
      181 gaatatattt atttaaagat aattgcgttt ttattgtcag ggagtgagtt tgcttaaaaa
      241 ctcgtttaga tccactagct taattaagca tgcactagat aacttcgtat agcatacatt
      301 atacgaagtt atagcttctg atggaattag aacttggcaa aacaatactg agaatgaagt
      361 gtatgtggaa cagaggctgc tgatctcgtt cttcaggcta tgaaactgac acatttggaa
      421 accacagtac ttagaaccac aaagtgggaa tcaagagaaa aacaatgatc ccacgagaga
      481 tcgaattcta gtatgtatgt aagttaataa aacccatttt tgcggaaagt agataaaaaa
      541 aacatttttt ttttttactg cactggatat cattgaactt atctgatcag ttttaaattt
      601 acttcgatcc aagggtattt gatgtaccag gttctttcga ttacctctca ctcaaaatga
      661 cattccactc aaagtcagcg ctgtttgcct ccttctctgt ccacagaaat atcgccgtct
      721 ctttcgccgc tgcgtccgct atctctttcg ccaccgtttg tagcgttacg tagcgtcaat
      781 gtccgccttc agttgcattt tgtcagcggt ttcgtgacga agctccaagc ggtttacgcc
      841 atcaattaaa cacaaagtgc tgtgcc
//
