LOCUS       sequence-32404-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pBPHLWL-VP16AD
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     169..187
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    372..405
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cagcgctgac tttgagtgga atgtcatttt gagtgagagg taatcgaaag aacctggtac
       61 atcaaatacc cttggatcga agtaaattta aaactgatca gataagttca atgatatcca
      121 gtgcagtaaa aaaaaaaaat gtttttttta tctactttcc gcaaaaatgg gttttattaa
      181 cttacataca tactagaatt cgatctctcg tgggatcatt gtttttctct tgattcccac
      241 tttgtggttc taagtactgt ggtttccaaa tgtgtcagtt tcatagcctg aagaacgaga
      301 tcagcagcct ctgttccaca tacacttcat tctcagtatt gttttgccaa gttctaattc
      361 catcagaagc tataacttcg tataatgtat gctatacgaa gttatctagt gcatgcttaa
      421 ttaagctagt ggatctaaac gagtttttaa gcaaactcac tccctgacaa taaaaacgca
      481 attatcttta aataaatata ttctatttat taaccaagta gctaatatag ctaaaatcaa
      541 tttgttgcta acttaaaact aacataatta tgtattagtt atttacattc ttttggttgg
      601 ttacaaccaa ataaataata tcacttttaa aaacttaagc caggaactga ataattttct
      661 atttggcttt agtcgacggt atcgatctag aggcgcgcct tacttgccgc cgcccagggg
      721 gccgtagcgg gtgcggttct ggctcacgcg gttgcgcagg cgctgcacgc gctggcgcag
      781 ctcggccacg cgggtctcca gggcggtgtt ctcgcgctcc a
//
